Detection of smell print differences between nonmalignant and malignant prostate cells with an electronic nose

Antti Roine (Corresponding Author), Mikko Tolvanen, Miki Sipiläinen, Pekka Kumpulainen, Merja A. Helenius, Terho Lehtimäki, Jouko Vepsäläinen, Tuomo A. Keinänen, Merja R. Häkkinen, Juha Koskimäki, Erik Veskimäe, Antti Tuokko, Tapio Visakorpi, Teuvo L. Tammela, Thanos Sioris, Timo Paavonen, Jukka Lekkala, Hannu Helle, Niku K.J. Oksala

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

Aim: To determine whether an electronic nose can differentiate cultured nonmalignant and malignant prostatic cells from each other and whether the smell print is secreted to the surrounding medium. Materials & methods: Prostatic nonmalignant (EP-156T and controls) and malignant (LNCaP) cell lines, as well as conditioned and unconditioned media, were collected. The smell prints of the samples were analyzed by a ChemPro® 100 electronic nose device. The data were normalized and dimension reduction was conducted. The samples were classified and misclassification rates were calculated. Results: The electronic nose differentiated the nonmalignant and malignant cell lines from each other, achieving misclassification rates of 2.9–3.6%. Cells did not differ from the conditioned medium but differed from the unconditioned medium (misclassification rates: 0.0–25.6%). Conclusion: Malignant and nonmalignant prostatic cell lines have distinct smell prints. Prostatic cancer cells seem to modify the smell print of their medium.
Original languageEnglish
Pages (from-to)1157-1165
JournalFuture Oncology
Volume8
Issue number9
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

Fingerprint

Electronic Nose
Smell
Prostate
Conditioned Culture Medium
Cell Line
Prostatic Neoplasms
Equipment and Supplies

Keywords

  • Cell lines
  • electronic noise
  • machine olfaction
  • olfactory detection
  • prostate cancer
  • volatile organic compounds

Cite this

Roine, A., Tolvanen, M., Sipiläinen, M., Kumpulainen, P., Helenius, M. A., Lehtimäki, T., ... Oksala, N. K. J. (2012). Detection of smell print differences between nonmalignant and malignant prostate cells with an electronic nose. Future Oncology, 8(9), 1157-1165. https://doi.org/10.2217/fon.12.93
Roine, Antti ; Tolvanen, Mikko ; Sipiläinen, Miki ; Kumpulainen, Pekka ; Helenius, Merja A. ; Lehtimäki, Terho ; Vepsäläinen, Jouko ; Keinänen, Tuomo A. ; Häkkinen, Merja R. ; Koskimäki, Juha ; Veskimäe, Erik ; Tuokko, Antti ; Visakorpi, Tapio ; Tammela, Teuvo L. ; Sioris, Thanos ; Paavonen, Timo ; Lekkala, Jukka ; Helle, Hannu ; Oksala, Niku K.J. / Detection of smell print differences between nonmalignant and malignant prostate cells with an electronic nose. In: Future Oncology. 2012 ; Vol. 8, No. 9. pp. 1157-1165.
@article{6b48ed9a2a3e4c628d821e4a15f38378,
title = "Detection of smell print differences between nonmalignant and malignant prostate cells with an electronic nose",
abstract = "Aim: To determine whether an electronic nose can differentiate cultured nonmalignant and malignant prostatic cells from each other and whether the smell print is secreted to the surrounding medium. Materials & methods: Prostatic nonmalignant (EP-156T and controls) and malignant (LNCaP) cell lines, as well as conditioned and unconditioned media, were collected. The smell prints of the samples were analyzed by a ChemPro{\circledR} 100 electronic nose device. The data were normalized and dimension reduction was conducted. The samples were classified and misclassification rates were calculated. Results: The electronic nose differentiated the nonmalignant and malignant cell lines from each other, achieving misclassification rates of 2.9–3.6{\%}. Cells did not differ from the conditioned medium but differed from the unconditioned medium (misclassification rates: 0.0–25.6{\%}). Conclusion: Malignant and nonmalignant prostatic cell lines have distinct smell prints. Prostatic cancer cells seem to modify the smell print of their medium.",
keywords = "Cell lines, electronic noise, machine olfaction, olfactory detection, prostate cancer, volatile organic compounds",
author = "Antti Roine and Mikko Tolvanen and Miki Sipil{\"a}inen and Pekka Kumpulainen and Helenius, {Merja A.} and Terho Lehtim{\"a}ki and Jouko Veps{\"a}l{\"a}inen and Kein{\"a}nen, {Tuomo A.} and H{\"a}kkinen, {Merja R.} and Juha Koskim{\"a}ki and Erik Veskim{\"a}e and Antti Tuokko and Tapio Visakorpi and Tammela, {Teuvo L.} and Thanos Sioris and Timo Paavonen and Jukka Lekkala and Hannu Helle and Oksala, {Niku K.J.}",
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Roine, A, Tolvanen, M, Sipiläinen, M, Kumpulainen, P, Helenius, MA, Lehtimäki, T, Vepsäläinen, J, Keinänen, TA, Häkkinen, MR, Koskimäki, J, Veskimäe, E, Tuokko, A, Visakorpi, T, Tammela, TL, Sioris, T, Paavonen, T, Lekkala, J, Helle, H & Oksala, NKJ 2012, 'Detection of smell print differences between nonmalignant and malignant prostate cells with an electronic nose', Future Oncology, vol. 8, no. 9, pp. 1157-1165. https://doi.org/10.2217/fon.12.93

Detection of smell print differences between nonmalignant and malignant prostate cells with an electronic nose. / Roine, Antti (Corresponding Author); Tolvanen, Mikko; Sipiläinen, Miki; Kumpulainen, Pekka; Helenius, Merja A.; Lehtimäki, Terho; Vepsäläinen, Jouko; Keinänen, Tuomo A.; Häkkinen, Merja R.; Koskimäki, Juha; Veskimäe, Erik; Tuokko, Antti; Visakorpi, Tapio; Tammela, Teuvo L.; Sioris, Thanos; Paavonen, Timo; Lekkala, Jukka; Helle, Hannu; Oksala, Niku K.J.

In: Future Oncology, Vol. 8, No. 9, 2012, p. 1157-1165.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Detection of smell print differences between nonmalignant and malignant prostate cells with an electronic nose

AU - Roine, Antti

AU - Tolvanen, Mikko

AU - Sipiläinen, Miki

AU - Kumpulainen, Pekka

AU - Helenius, Merja A.

AU - Lehtimäki, Terho

AU - Vepsäläinen, Jouko

AU - Keinänen, Tuomo A.

AU - Häkkinen, Merja R.

AU - Koskimäki, Juha

AU - Veskimäe, Erik

AU - Tuokko, Antti

AU - Visakorpi, Tapio

AU - Tammela, Teuvo L.

AU - Sioris, Thanos

AU - Paavonen, Timo

AU - Lekkala, Jukka

AU - Helle, Hannu

AU - Oksala, Niku K.J.

PY - 2012

Y1 - 2012

N2 - Aim: To determine whether an electronic nose can differentiate cultured nonmalignant and malignant prostatic cells from each other and whether the smell print is secreted to the surrounding medium. Materials & methods: Prostatic nonmalignant (EP-156T and controls) and malignant (LNCaP) cell lines, as well as conditioned and unconditioned media, were collected. The smell prints of the samples were analyzed by a ChemPro® 100 electronic nose device. The data were normalized and dimension reduction was conducted. The samples were classified and misclassification rates were calculated. Results: The electronic nose differentiated the nonmalignant and malignant cell lines from each other, achieving misclassification rates of 2.9–3.6%. Cells did not differ from the conditioned medium but differed from the unconditioned medium (misclassification rates: 0.0–25.6%). Conclusion: Malignant and nonmalignant prostatic cell lines have distinct smell prints. Prostatic cancer cells seem to modify the smell print of their medium.

AB - Aim: To determine whether an electronic nose can differentiate cultured nonmalignant and malignant prostatic cells from each other and whether the smell print is secreted to the surrounding medium. Materials & methods: Prostatic nonmalignant (EP-156T and controls) and malignant (LNCaP) cell lines, as well as conditioned and unconditioned media, were collected. The smell prints of the samples were analyzed by a ChemPro® 100 electronic nose device. The data were normalized and dimension reduction was conducted. The samples were classified and misclassification rates were calculated. Results: The electronic nose differentiated the nonmalignant and malignant cell lines from each other, achieving misclassification rates of 2.9–3.6%. Cells did not differ from the conditioned medium but differed from the unconditioned medium (misclassification rates: 0.0–25.6%). Conclusion: Malignant and nonmalignant prostatic cell lines have distinct smell prints. Prostatic cancer cells seem to modify the smell print of their medium.

KW - Cell lines

KW - electronic noise

KW - machine olfaction

KW - olfactory detection

KW - prostate cancer

KW - volatile organic compounds

U2 - 10.2217/fon.12.93

DO - 10.2217/fon.12.93

M3 - Article

VL - 8

SP - 1157

EP - 1165

JO - Future Oncology

JF - Future Oncology

SN - 1479-6694

IS - 9

ER -

Roine A, Tolvanen M, Sipiläinen M, Kumpulainen P, Helenius MA, Lehtimäki T et al. Detection of smell print differences between nonmalignant and malignant prostate cells with an electronic nose. Future Oncology. 2012;8(9):1157-1165. https://doi.org/10.2217/fon.12.93