Detection of white-rot fungi by a non-toxic stain

Marja-Leena Niku-Paavola; Laura Raaska; Merja Itävaara

doi:10.1016/S0953-7562(09)81260-4

Detection of white-rot fungi by a non-toxic stain

Marja-Leena Niku-Paavola, Laura Raaska, Merja Itävaara

VTT Technical Research Centre of Finland

Research output: Contribution to journal › Article › Scientific › peer-review

251 Citations (Scopus)

Abstract

A method is described for distinguishing between white-rot fungi producing laccase (EC 1 · 10 · 3 · 2) and brown-rot fungi producing catechol oxidase (EC 1 · 10 · 3 · 1). Laccase oxidizes a redox indicator ABTS (2, 2'-azino-di(3-ethylbenzothiazoline-6-sulfonic acid)) to form a blue-green cation radical whilst catechol oxidase does not. The method is suitable for detecting laccase excreted by mycelia growing on agar and wood-based substrates, and proteins derived from culture liquids after electrophoretic separation on polyacrylamide gel. The method can be adapted for quantitative estimation by spectrophotometry.

Original language	English
Pages (from-to)	27-31
Journal	Mycological Research
Volume	94
Issue number	1
DOIs	https://doi.org/10.1016/S0953-7562(09)81260-4
Publication status	Published - 1990
MoE publication type	A1 Journal article-refereed

Keywords

laccase
white-rot fungi
wnzymatic stain
wood-rotting fungi
identification
isoenzyme

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10.1016/S0953-7562(09)81260-4

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title = "Detection of white-rot fungi by a non-toxic stain",

abstract = "A method is described for distinguishing between white-rot fungi producing laccase (EC 1 · 10 · 3 · 2) and brown-rot fungi producing catechol oxidase (EC 1 · 10 · 3 · 1). Laccase oxidizes a redox indicator ABTS (2, 2'-azino-di(3-ethylbenzothiazoline-6-sulfonic acid)) to form a blue-green cation radical whilst catechol oxidase does not. The method is suitable for detecting laccase excreted by mycelia growing on agar and wood-based substrates, and proteins derived from culture liquids after electrophoretic separation on polyacrylamide gel. The method can be adapted for quantitative estimation by spectrophotometry.",

keywords = "laccase, white-rot fungi, wnzymatic stain, wood-rotting fungi, identification, isoenzyme",

author = "Marja-Leena Niku-Paavola and Laura Raaska and Merja It{\"a}vaara",

year = "1990",

doi = "10.1016/S0953-7562(09)81260-4",

language = "English",

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pages = "27--31",

journal = "Mycological Research",

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T1 - Detection of white-rot fungi by a non-toxic stain

AU - Niku-Paavola, Marja-Leena

AU - Raaska, Laura

AU - Itävaara, Merja

PY - 1990

Y1 - 1990

N2 - A method is described for distinguishing between white-rot fungi producing laccase (EC 1 · 10 · 3 · 2) and brown-rot fungi producing catechol oxidase (EC 1 · 10 · 3 · 1). Laccase oxidizes a redox indicator ABTS (2, 2'-azino-di(3-ethylbenzothiazoline-6-sulfonic acid)) to form a blue-green cation radical whilst catechol oxidase does not. The method is suitable for detecting laccase excreted by mycelia growing on agar and wood-based substrates, and proteins derived from culture liquids after electrophoretic separation on polyacrylamide gel. The method can be adapted for quantitative estimation by spectrophotometry.

AB - A method is described for distinguishing between white-rot fungi producing laccase (EC 1 · 10 · 3 · 2) and brown-rot fungi producing catechol oxidase (EC 1 · 10 · 3 · 1). Laccase oxidizes a redox indicator ABTS (2, 2'-azino-di(3-ethylbenzothiazoline-6-sulfonic acid)) to form a blue-green cation radical whilst catechol oxidase does not. The method is suitable for detecting laccase excreted by mycelia growing on agar and wood-based substrates, and proteins derived from culture liquids after electrophoretic separation on polyacrylamide gel. The method can be adapted for quantitative estimation by spectrophotometry.

KW - laccase

KW - white-rot fungi

KW - wnzymatic stain

KW - wood-rotting fungi

KW - identification

KW - isoenzyme

U2 - 10.1016/S0953-7562(09)81260-4

DO - 10.1016/S0953-7562(09)81260-4

M3 - Article

SN - 0953-7562

VL - 94

SP - 27

EP - 31

JO - Mycological Research

JF - Mycological Research

IS - 1

ER -

Detection of white-rot fungi by a non-toxic stain

Abstract

Keywords

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