Detection of white-rot fungi by a non-toxic stain

Marja-Leena Niku-Paavola, Laura Raaska, Merja Itävaara

Research output: Contribution to journalArticleScientificpeer-review

212 Citations (Scopus)

Abstract

A method is described for distinguishing between white-rot fungi producing laccase (EC 1 · 10 · 3 · 2) and brown-rot fungi producing catechol oxidase (EC 1 · 10 · 3 · 1). Laccase oxidizes a redox indicator ABTS (2, 2'-azino-di(3-ethylbenzothiazoline-6-sulfonic acid)) to form a blue-green cation radical whilst catechol oxidase does not. The method is suitable for detecting laccase excreted by mycelia growing on agar and wood-based substrates, and proteins derived from culture liquids after electrophoretic separation on polyacrylamide gel. The method can be adapted for quantitative estimation by spectrophotometry.
Original languageEnglish
Pages (from-to)27-31
JournalMycological Research
Volume94
Issue number1
DOIs
Publication statusPublished - 1990
MoE publication typeA1 Journal article-refereed

Fingerprint

Laccase
white-rot fungi
laccase
Catechol Oxidase
Fungi
Coloring Agents
oxidoreductases
fungus
catechol oxidase
brown-rot fungi
Spectrophotometry
sulfonic acid
Mycelium
polyacrylamide
spectrophotometry
agar
mycelium
Agar
Oxidation-Reduction
Cations

Keywords

  • laccase
  • white-rot fungi
  • wnzymatic stain
  • wood-rotting fungi
  • identification
  • isoenzyme

Cite this

Niku-Paavola, Marja-Leena ; Raaska, Laura ; Itävaara, Merja. / Detection of white-rot fungi by a non-toxic stain. In: Mycological Research. 1990 ; Vol. 94, No. 1. pp. 27-31.
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Niku-Paavola, M-L, Raaska, L & Itävaara, M 1990, 'Detection of white-rot fungi by a non-toxic stain', Mycological Research, vol. 94, no. 1, pp. 27-31. https://doi.org/10.1016/S0953-7562(09)81260-4

Detection of white-rot fungi by a non-toxic stain. / Niku-Paavola, Marja-Leena; Raaska, Laura; Itävaara, Merja.

In: Mycological Research, Vol. 94, No. 1, 1990, p. 27-31.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Detection of white-rot fungi by a non-toxic stain

AU - Niku-Paavola, Marja-Leena

AU - Raaska, Laura

AU - Itävaara, Merja

PY - 1990

Y1 - 1990

N2 - A method is described for distinguishing between white-rot fungi producing laccase (EC 1 · 10 · 3 · 2) and brown-rot fungi producing catechol oxidase (EC 1 · 10 · 3 · 1). Laccase oxidizes a redox indicator ABTS (2, 2'-azino-di(3-ethylbenzothiazoline-6-sulfonic acid)) to form a blue-green cation radical whilst catechol oxidase does not. The method is suitable for detecting laccase excreted by mycelia growing on agar and wood-based substrates, and proteins derived from culture liquids after electrophoretic separation on polyacrylamide gel. The method can be adapted for quantitative estimation by spectrophotometry.

AB - A method is described for distinguishing between white-rot fungi producing laccase (EC 1 · 10 · 3 · 2) and brown-rot fungi producing catechol oxidase (EC 1 · 10 · 3 · 1). Laccase oxidizes a redox indicator ABTS (2, 2'-azino-di(3-ethylbenzothiazoline-6-sulfonic acid)) to form a blue-green cation radical whilst catechol oxidase does not. The method is suitable for detecting laccase excreted by mycelia growing on agar and wood-based substrates, and proteins derived from culture liquids after electrophoretic separation on polyacrylamide gel. The method can be adapted for quantitative estimation by spectrophotometry.

KW - laccase

KW - white-rot fungi

KW - wnzymatic stain

KW - wood-rotting fungi

KW - identification

KW - isoenzyme

U2 - 10.1016/S0953-7562(09)81260-4

DO - 10.1016/S0953-7562(09)81260-4

M3 - Article

VL - 94

SP - 27

EP - 31

JO - Fungal Biology

JF - Fungal Biology

SN - 1878-6146

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