Detection of white-rot fungi by a non-toxic stain

Marja-Leena Niku-Paavola, Laura Raaska, Merja Itävaara

Research output: Contribution to journalArticleScientificpeer-review

223 Citations (Scopus)

Abstract

A method is described for distinguishing between white-rot fungi producing laccase (EC 1 · 10 · 3 · 2) and brown-rot fungi producing catechol oxidase (EC 1 · 10 · 3 · 1). Laccase oxidizes a redox indicator ABTS (2, 2'-azino-di(3-ethylbenzothiazoline-6-sulfonic acid)) to form a blue-green cation radical whilst catechol oxidase does not. The method is suitable for detecting laccase excreted by mycelia growing on agar and wood-based substrates, and proteins derived from culture liquids after electrophoretic separation on polyacrylamide gel. The method can be adapted for quantitative estimation by spectrophotometry.
Original languageEnglish
Pages (from-to)27-31
JournalMycological Research
Volume94
Issue number1
DOIs
Publication statusPublished - 1990
MoE publication typeA1 Journal article-refereed

Keywords

  • laccase
  • white-rot fungi
  • wnzymatic stain
  • wood-rotting fungi
  • identification
  • isoenzyme

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