Abstract
Transverse relaxation-optimized NMR experiment (TROSY) for the measurement of three-bond scalar coupling constant between 1Hαi−1 and 15Ni defining the dihedral angle ψ is described. The triple-spin-state-selective experiment allows measurement of 3JHαN from 13Cα, 15N, and 1HN correlation spectra H2O with minimum resonance overlap. Transverse relaxation of 13Cα spin is minimized by using spin-state-selective filtering and by acquiring a signal longer in 15N-dimension in a manner of semi-constant-time TROSY evolution. The 3JHαN values obtained with the proposed α/β-HN(CO)CA-J TROSY scheme are in good agreement with the values measured earlier from ubiquitin in D2O using the HCACO[N] experiment.
| Original language | English |
|---|---|
| Pages (from-to) | 255 - 259 |
| Number of pages | 5 |
| Journal | Journal of Magnetic Resonance |
| Volume | 146 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2000 |
| MoE publication type | A1 Journal article-refereed |
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Determination of backbone angle ψ in proteins using a TROSY-based α/β-HN(CO)CA-J experiment. / Permi, P. (Corresponding Author); Kilpeläinen, I.; Annila, Arto.
In: Journal of Magnetic Resonance, Vol. 146, No. 2, 2000, p. 255 - 259.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Determination of backbone angle ψ in proteins using a TROSY-based α/β-HN(CO)CA-J experiment
AU - Permi, P.
AU - Kilpeläinen, I.
AU - Annila, Arto
PY - 2000
Y1 - 2000
N2 - Transverse relaxation-optimized NMR experiment (TROSY) for the measurement of three-bond scalar coupling constant between 1Hαi−1 and 15Ni defining the dihedral angle ψ is described. The triple-spin-state-selective experiment allows measurement of 3JHαN from 13Cα, 15N, and 1HN correlation spectra H2O with minimum resonance overlap. Transverse relaxation of 13Cα spin is minimized by using spin-state-selective filtering and by acquiring a signal longer in 15N-dimension in a manner of semi-constant-time TROSY evolution. The 3JHαN values obtained with the proposed α/β-HN(CO)CA-J TROSY scheme are in good agreement with the values measured earlier from ubiquitin in D2O using the HCACO[N] experiment.
AB - Transverse relaxation-optimized NMR experiment (TROSY) for the measurement of three-bond scalar coupling constant between 1Hαi−1 and 15Ni defining the dihedral angle ψ is described. The triple-spin-state-selective experiment allows measurement of 3JHαN from 13Cα, 15N, and 1HN correlation spectra H2O with minimum resonance overlap. Transverse relaxation of 13Cα spin is minimized by using spin-state-selective filtering and by acquiring a signal longer in 15N-dimension in a manner of semi-constant-time TROSY evolution. The 3JHαN values obtained with the proposed α/β-HN(CO)CA-J TROSY scheme are in good agreement with the values measured earlier from ubiquitin in D2O using the HCACO[N] experiment.
U2 - 10.1006/jmre.2000.2157
DO - 10.1006/jmre.2000.2157
M3 - Article
VL - 146
SP - 255
EP - 259
JO - Journal of Magnetic Resonance
JF - Journal of Magnetic Resonance
SN - 1090-7807
IS - 2
ER -