Determination of hexenuronic acid in softwood kraft pulps by three different methods

Maija Tenkanen, G. Gellerstedt, Tapani Vuorinen, Anita Teleman, Marjukka Perttula, J. Li, Johanna Buchert

Research output: Contribution to journalArticleScientificpeer-review

110 Citations (Scopus)

Abstract

In this paper; three methods for the detection of hexenuronic acid (HexA) in unbleached nan bleached softwood krafts pulps were compared The methods were based on total enzymatic hydrolysis of pulp combined,with high-performance anion-exchange chromatography of the liberated HexA-substituted xylo-oligosaccharides (the VTT method), selective acid hydrolysis of pup combined with UV spectroscopy of the hydrolysis products (the HUT method), and selective mercuric acetate hydrolysis of pulp combined,with colorimetric determination of the hydrolysis products (the KTH method). The results obtained by the three methods correlated well with each other. The highest values were obtained with the KTH method and the values obtained with the VTT mid HUT methods were 90-95% and 75-86% of the the KTH values, respectively. None of the methods was clearly superior to the other two in the analysis of HexA. However, the VTT method allows simultaneous quantification of 4-O-methyliduronic acid and 4-O-methyliduronic acid along,with the analysis of HexA.
Original languageEnglish
Pages (from-to)306-311
Number of pages6
JournalJournal of Pulp and Paper Science
Volume25
Issue number9
Publication statusPublished - 1999
MoE publication typeA1 Journal article-refereed

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Kraft pulp
Softwoods
Hydrolysis
Pulp
Acids
Enzymatic hydrolysis
Chromatography
Ultraviolet spectroscopy
Oligosaccharides
Anions
hexenuronic acid
Ion exchange
Negative ions

Cite this

Tenkanen, M., Gellerstedt, G., Vuorinen, T., Teleman, A., Perttula, M., Li, J., & Buchert, J. (1999). Determination of hexenuronic acid in softwood kraft pulps by three different methods. Journal of Pulp and Paper Science, 25(9), 306-311.
Tenkanen, Maija ; Gellerstedt, G. ; Vuorinen, Tapani ; Teleman, Anita ; Perttula, Marjukka ; Li, J. ; Buchert, Johanna. / Determination of hexenuronic acid in softwood kraft pulps by three different methods. In: Journal of Pulp and Paper Science. 1999 ; Vol. 25, No. 9. pp. 306-311.
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abstract = "In this paper; three methods for the detection of hexenuronic acid (HexA) in unbleached nan bleached softwood krafts pulps were compared The methods were based on total enzymatic hydrolysis of pulp combined,with high-performance anion-exchange chromatography of the liberated HexA-substituted xylo-oligosaccharides (the VTT method), selective acid hydrolysis of pup combined with UV spectroscopy of the hydrolysis products (the HUT method), and selective mercuric acetate hydrolysis of pulp combined,with colorimetric determination of the hydrolysis products (the KTH method). The results obtained by the three methods correlated well with each other. The highest values were obtained with the KTH method and the values obtained with the VTT mid HUT methods were 90-95{\%} and 75-86{\%} of the the KTH values, respectively. None of the methods was clearly superior to the other two in the analysis of HexA. However, the VTT method allows simultaneous quantification of 4-O-methyliduronic acid and 4-O-methyliduronic acid along,with the analysis of HexA.",
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Tenkanen, M, Gellerstedt, G, Vuorinen, T, Teleman, A, Perttula, M, Li, J & Buchert, J 1999, 'Determination of hexenuronic acid in softwood kraft pulps by three different methods', Journal of Pulp and Paper Science, vol. 25, no. 9, pp. 306-311.

Determination of hexenuronic acid in softwood kraft pulps by three different methods. / Tenkanen, Maija; Gellerstedt, G.; Vuorinen, Tapani; Teleman, Anita; Perttula, Marjukka; Li, J.; Buchert, Johanna.

In: Journal of Pulp and Paper Science, Vol. 25, No. 9, 1999, p. 306-311.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Determination of hexenuronic acid in softwood kraft pulps by three different methods

AU - Tenkanen, Maija

AU - Gellerstedt, G.

AU - Vuorinen, Tapani

AU - Teleman, Anita

AU - Perttula, Marjukka

AU - Li, J.

AU - Buchert, Johanna

PY - 1999

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N2 - In this paper; three methods for the detection of hexenuronic acid (HexA) in unbleached nan bleached softwood krafts pulps were compared The methods were based on total enzymatic hydrolysis of pulp combined,with high-performance anion-exchange chromatography of the liberated HexA-substituted xylo-oligosaccharides (the VTT method), selective acid hydrolysis of pup combined with UV spectroscopy of the hydrolysis products (the HUT method), and selective mercuric acetate hydrolysis of pulp combined,with colorimetric determination of the hydrolysis products (the KTH method). The results obtained by the three methods correlated well with each other. The highest values were obtained with the KTH method and the values obtained with the VTT mid HUT methods were 90-95% and 75-86% of the the KTH values, respectively. None of the methods was clearly superior to the other two in the analysis of HexA. However, the VTT method allows simultaneous quantification of 4-O-methyliduronic acid and 4-O-methyliduronic acid along,with the analysis of HexA.

AB - In this paper; three methods for the detection of hexenuronic acid (HexA) in unbleached nan bleached softwood krafts pulps were compared The methods were based on total enzymatic hydrolysis of pulp combined,with high-performance anion-exchange chromatography of the liberated HexA-substituted xylo-oligosaccharides (the VTT method), selective acid hydrolysis of pup combined with UV spectroscopy of the hydrolysis products (the HUT method), and selective mercuric acetate hydrolysis of pulp combined,with colorimetric determination of the hydrolysis products (the KTH method). The results obtained by the three methods correlated well with each other. The highest values were obtained with the KTH method and the values obtained with the VTT mid HUT methods were 90-95% and 75-86% of the the KTH values, respectively. None of the methods was clearly superior to the other two in the analysis of HexA. However, the VTT method allows simultaneous quantification of 4-O-methyliduronic acid and 4-O-methyliduronic acid along,with the analysis of HexA.

M3 - Article

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JO - J-FOR: Journal of Science & Technology for Forest Products and Processes

JF - J-FOR: Journal of Science & Technology for Forest Products and Processes

SN - 1927-6311

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ER -

Tenkanen M, Gellerstedt G, Vuorinen T, Teleman A, Perttula M, Li J et al. Determination of hexenuronic acid in softwood kraft pulps by three different methods. Journal of Pulp and Paper Science. 1999;25(9):306-311.