Determination of Catharanthus roseus alkaloids by high-performance liquid chromatography-isotope dilution thermospray-mass spectrometry

Seppo Auriola (Corresponding Author), Toivo Naaranlahti, Seppo Lapinjoki

Research output: Contribution to journalArticleScientificpeer-review

16 Citations (Scopus)

Abstract

This paper describes a high-performance liquid chromatography-isotope dilution thermospray-mass spectrometry method for the determination of catharanthine, tabersonine, yohimbine and ajmalicine in cell culture samples of Catharanthus roseus. The use of deuterated analogues of the alkaloids as internal standards remarkably improved the precision of the analysis. The standard deviation between six repeated assays was close to or below 10% when the reference compounds were analyzed, but was about 10% higher, in most instances, when vincamine was used as the internal standard. The precision of this method was about 20% for biological extracts.

Original languageEnglish
Pages (from-to)227 - 231
Number of pages5
JournalJournal of Chromatography A
Volume554
Issue number1-2
DOIs
Publication statusPublished - 1991
MoE publication typeA1 Journal article-refereed

Fingerprint

Catharanthus
High performance liquid chromatography
Alkaloids
Isotopes
Dilution
Mass spectrometry
Mass Spectrometry
Vincamine
High Pressure Liquid Chromatography
Yohimbine
Cell culture
Assays
Cell Culture Techniques
catharanthine
tabersonine
raubasine

Cite this

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title = "Determination of Catharanthus roseus alkaloids by high-performance liquid chromatography-isotope dilution thermospray-mass spectrometry",
abstract = "This paper describes a high-performance liquid chromatography-isotope dilution thermospray-mass spectrometry method for the determination of catharanthine, tabersonine, yohimbine and ajmalicine in cell culture samples of Catharanthus roseus. The use of deuterated analogues of the alkaloids as internal standards remarkably improved the precision of the analysis. The standard deviation between six repeated assays was close to or below 10{\%} when the reference compounds were analyzed, but was about 10{\%} higher, in most instances, when vincamine was used as the internal standard. The precision of this method was about 20{\%} for biological extracts.",
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Determination of Catharanthus roseus alkaloids by high-performance liquid chromatography-isotope dilution thermospray-mass spectrometry. / Auriola, Seppo (Corresponding Author); Naaranlahti, Toivo; Lapinjoki, Seppo.

In: Journal of Chromatography A, Vol. 554, No. 1-2, 1991, p. 227 - 231.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Determination of Catharanthus roseus alkaloids by high-performance liquid chromatography-isotope dilution thermospray-mass spectrometry

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AU - Naaranlahti, Toivo

AU - Lapinjoki, Seppo

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N2 - This paper describes a high-performance liquid chromatography-isotope dilution thermospray-mass spectrometry method for the determination of catharanthine, tabersonine, yohimbine and ajmalicine in cell culture samples of Catharanthus roseus. The use of deuterated analogues of the alkaloids as internal standards remarkably improved the precision of the analysis. The standard deviation between six repeated assays was close to or below 10% when the reference compounds were analyzed, but was about 10% higher, in most instances, when vincamine was used as the internal standard. The precision of this method was about 20% for biological extracts.

AB - This paper describes a high-performance liquid chromatography-isotope dilution thermospray-mass spectrometry method for the determination of catharanthine, tabersonine, yohimbine and ajmalicine in cell culture samples of Catharanthus roseus. The use of deuterated analogues of the alkaloids as internal standards remarkably improved the precision of the analysis. The standard deviation between six repeated assays was close to or below 10% when the reference compounds were analyzed, but was about 10% higher, in most instances, when vincamine was used as the internal standard. The precision of this method was about 20% for biological extracts.

U2 - 10.1016/S0021-9673(01)88452-1

DO - 10.1016/S0021-9673(01)88452-1

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JO - Journal of Chromatography A

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