Determination of ice crystal damage of high pressure frozen foods by light microscopy and image analysis

Martina Lille, Karin Autio

Research output: Contribution to conferenceConference articleScientific

Abstract

Two different methods were developed for studying ice crystal damage. Wheat starch gels and wheat dough were high pressure frozen and conventionally frozen in plastic, cylindrical tubes (inner diameter 15 mm). Cryosections (diameter the same as the plastic tube) with a thickness of 15 µm were cut from two different positions of the frozen samples. Sections were stained with iodine and examined in brightfield. Image analysis was used to determine the amount and area of pores. Total analysed area was about 85 mm2/ section. The damage caused by freezing was very much dependent on freezing method. Pressure-shift freezing at 200 MPa caused less damage than conventional freezing. Another method based on embedding was developed for frozen and thawed potato and carrot samples. Freezing caused separation of the cells in the studied vegetables. This was also demonstrated by confocal laser scanning microscopy. The area of the spaces between the cells was determined by image analysis.
Original languageEnglish
Publication statusPublished - 2004
EventConference on Food Structure and Food Quality - Cork, Ireland
Duration: 3 Oct 20047 Oct 2004

Conference

ConferenceConference on Food Structure and Food Quality
CountryIreland
CityCork
Period3/10/047/10/04

Fingerprint

frozen foods
crystals
light microscopy
freezing
ice
image analysis
plastics
wheat starch
starch gels
confocal laser scanning microscopy
iodine
dough
carrots
vegetables
potatoes
cells
sampling
wheat
methodology

Cite this

Lille, M., & Autio, K. (2004). Determination of ice crystal damage of high pressure frozen foods by light microscopy and image analysis. Paper presented at Conference on Food Structure and Food Quality, Cork, Ireland.
Lille, Martina ; Autio, Karin. / Determination of ice crystal damage of high pressure frozen foods by light microscopy and image analysis. Paper presented at Conference on Food Structure and Food Quality, Cork, Ireland.
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title = "Determination of ice crystal damage of high pressure frozen foods by light microscopy and image analysis",
abstract = "Two different methods were developed for studying ice crystal damage. Wheat starch gels and wheat dough were high pressure frozen and conventionally frozen in plastic, cylindrical tubes (inner diameter 15 mm). Cryosections (diameter the same as the plastic tube) with a thickness of 15 µm were cut from two different positions of the frozen samples. Sections were stained with iodine and examined in brightfield. Image analysis was used to determine the amount and area of pores. Total analysed area was about 85 mm2/ section. The damage caused by freezing was very much dependent on freezing method. Pressure-shift freezing at 200 MPa caused less damage than conventional freezing. Another method based on embedding was developed for frozen and thawed potato and carrot samples. Freezing caused separation of the cells in the studied vegetables. This was also demonstrated by confocal laser scanning microscopy. The area of the spaces between the cells was determined by image analysis.",
author = "Martina Lille and Karin Autio",
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language = "English",
note = "Conference on Food Structure and Food Quality ; Conference date: 03-10-2004 Through 07-10-2004",

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Lille, M & Autio, K 2004, 'Determination of ice crystal damage of high pressure frozen foods by light microscopy and image analysis' Paper presented at Conference on Food Structure and Food Quality, Cork, Ireland, 3/10/04 - 7/10/04, .

Determination of ice crystal damage of high pressure frozen foods by light microscopy and image analysis. / Lille, Martina; Autio, Karin.

2004. Paper presented at Conference on Food Structure and Food Quality, Cork, Ireland.

Research output: Contribution to conferenceConference articleScientific

TY - CONF

T1 - Determination of ice crystal damage of high pressure frozen foods by light microscopy and image analysis

AU - Lille, Martina

AU - Autio, Karin

PY - 2004

Y1 - 2004

N2 - Two different methods were developed for studying ice crystal damage. Wheat starch gels and wheat dough were high pressure frozen and conventionally frozen in plastic, cylindrical tubes (inner diameter 15 mm). Cryosections (diameter the same as the plastic tube) with a thickness of 15 µm were cut from two different positions of the frozen samples. Sections were stained with iodine and examined in brightfield. Image analysis was used to determine the amount and area of pores. Total analysed area was about 85 mm2/ section. The damage caused by freezing was very much dependent on freezing method. Pressure-shift freezing at 200 MPa caused less damage than conventional freezing. Another method based on embedding was developed for frozen and thawed potato and carrot samples. Freezing caused separation of the cells in the studied vegetables. This was also demonstrated by confocal laser scanning microscopy. The area of the spaces between the cells was determined by image analysis.

AB - Two different methods were developed for studying ice crystal damage. Wheat starch gels and wheat dough were high pressure frozen and conventionally frozen in plastic, cylindrical tubes (inner diameter 15 mm). Cryosections (diameter the same as the plastic tube) with a thickness of 15 µm were cut from two different positions of the frozen samples. Sections were stained with iodine and examined in brightfield. Image analysis was used to determine the amount and area of pores. Total analysed area was about 85 mm2/ section. The damage caused by freezing was very much dependent on freezing method. Pressure-shift freezing at 200 MPa caused less damage than conventional freezing. Another method based on embedding was developed for frozen and thawed potato and carrot samples. Freezing caused separation of the cells in the studied vegetables. This was also demonstrated by confocal laser scanning microscopy. The area of the spaces between the cells was determined by image analysis.

M3 - Conference article

ER -

Lille M, Autio K. Determination of ice crystal damage of high pressure frozen foods by light microscopy and image analysis. 2004. Paper presented at Conference on Food Structure and Food Quality, Cork, Ireland.