Determination of monosaccharide composition in plant fiber materials by capillary zone electrophoresis

Stella Rovio, Helena Simolin, Krista Koljonen, Heli Sirén (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

54 Citations (Scopus)

Abstract

The neutral sugar composition of acid hydrolyzed extracts of cellulose fiber samples, i.e. oat spelt, wheat straw, thermomechanica pulp (TMP) made of spruce, aspen stemwood, and bleached birch kraft pulp, was determined by a new capillary zone electrophoresis (CZE) method employing an alkaline background electrolyte. The method relies on in-capillary reaction and direct UV detection at wavelength 270 nm. Neutral carbohydrates d-(+)-galactose, d-(+)-glucose, l-rhamnose, d-(+)-mannose, d-(−)-arabinose, and d-(+)-xylose were simultaneously separated. The calibration plots were linear over a range from 10 to 150 mg/L for d-(+)-galactose, l-rhamnose, d-(+)-mannose, and d-(−)-arabinose and from 50 to 400 mg/L for d-(+)-glucose and d-(+)-xylose. Relative standard deviations (RSDs) of peak areas during a 5-day analysis period varied from 3.3% for galactose to 11.8% for rhamnose. RSDs of migration times varied between 0.3 and 0.7%. The detection limit (at S/N 3) was 5 mg/L for each monosaccharide. The results obtained by CZE agreed well with results obtained by high-performance anion-exchange chromatography. Glucose and xylose were the two predominant monosaccharides in the plants, except in the spruce TMP sample where glucose and mannose dominated.
Original languageEnglish
Pages (from-to)139-144
JournalJournal of Chromatography A
Volume1185
Issue number1
DOIs
Publication statusPublished - 2008
MoE publication typeA1 Journal article-refereed

Fingerprint

Rhamnose
Monosaccharides
Capillary Electrophoresis
Mannose
Electrophoresis
Galactose
Arabinose
Xylose
Glucose
Pulp
Fibers
Chemical analysis
Sugar Acids
Bleached pulp
Betula
Kraft pulp
Straw
Chromatography
Cellulose
Sugars

Keywords

  • Capillary electrophoresis
  • Monosaccharide
  • Hydrolysis
  • Plant
  • Direct UV detection
  • Wheat straw
  • Oat spelt
  • Spruce TMP
  • Aspen
  • Birch kraft pulp

Cite this

Rovio, Stella ; Simolin, Helena ; Koljonen, Krista ; Sirén, Heli. / Determination of monosaccharide composition in plant fiber materials by capillary zone electrophoresis. In: Journal of Chromatography A. 2008 ; Vol. 1185, No. 1. pp. 139-144.
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Determination of monosaccharide composition in plant fiber materials by capillary zone electrophoresis. / Rovio, Stella; Simolin, Helena; Koljonen, Krista; Sirén, Heli (Corresponding Author).

In: Journal of Chromatography A, Vol. 1185, No. 1, 2008, p. 139-144.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Determination of monosaccharide composition in plant fiber materials by capillary zone electrophoresis

AU - Rovio, Stella

AU - Simolin, Helena

AU - Koljonen, Krista

AU - Sirén, Heli

PY - 2008

Y1 - 2008

N2 - The neutral sugar composition of acid hydrolyzed extracts of cellulose fiber samples, i.e. oat spelt, wheat straw, thermomechanica pulp (TMP) made of spruce, aspen stemwood, and bleached birch kraft pulp, was determined by a new capillary zone electrophoresis (CZE) method employing an alkaline background electrolyte. The method relies on in-capillary reaction and direct UV detection at wavelength 270 nm. Neutral carbohydrates d-(+)-galactose, d-(+)-glucose, l-rhamnose, d-(+)-mannose, d-(−)-arabinose, and d-(+)-xylose were simultaneously separated. The calibration plots were linear over a range from 10 to 150 mg/L for d-(+)-galactose, l-rhamnose, d-(+)-mannose, and d-(−)-arabinose and from 50 to 400 mg/L for d-(+)-glucose and d-(+)-xylose. Relative standard deviations (RSDs) of peak areas during a 5-day analysis period varied from 3.3% for galactose to 11.8% for rhamnose. RSDs of migration times varied between 0.3 and 0.7%. The detection limit (at S/N 3) was 5 mg/L for each monosaccharide. The results obtained by CZE agreed well with results obtained by high-performance anion-exchange chromatography. Glucose and xylose were the two predominant monosaccharides in the plants, except in the spruce TMP sample where glucose and mannose dominated.

AB - The neutral sugar composition of acid hydrolyzed extracts of cellulose fiber samples, i.e. oat spelt, wheat straw, thermomechanica pulp (TMP) made of spruce, aspen stemwood, and bleached birch kraft pulp, was determined by a new capillary zone electrophoresis (CZE) method employing an alkaline background electrolyte. The method relies on in-capillary reaction and direct UV detection at wavelength 270 nm. Neutral carbohydrates d-(+)-galactose, d-(+)-glucose, l-rhamnose, d-(+)-mannose, d-(−)-arabinose, and d-(+)-xylose were simultaneously separated. The calibration plots were linear over a range from 10 to 150 mg/L for d-(+)-galactose, l-rhamnose, d-(+)-mannose, and d-(−)-arabinose and from 50 to 400 mg/L for d-(+)-glucose and d-(+)-xylose. Relative standard deviations (RSDs) of peak areas during a 5-day analysis period varied from 3.3% for galactose to 11.8% for rhamnose. RSDs of migration times varied between 0.3 and 0.7%. The detection limit (at S/N 3) was 5 mg/L for each monosaccharide. The results obtained by CZE agreed well with results obtained by high-performance anion-exchange chromatography. Glucose and xylose were the two predominant monosaccharides in the plants, except in the spruce TMP sample where glucose and mannose dominated.

KW - Capillary electrophoresis

KW - Monosaccharide

KW - Hydrolysis

KW - Plant

KW - Direct UV detection

KW - Wheat straw

KW - Oat spelt

KW - Spruce TMP

KW - Aspen

KW - Birch kraft pulp

U2 - 10.1016/j.chroma.2008.01.031

DO - 10.1016/j.chroma.2008.01.031

M3 - Article

VL - 1185

SP - 139

EP - 144

JO - Journal of Chromatography A

JF - Journal of Chromatography A

SN - 0021-9673

IS - 1

ER -