Determination of monosaccharide composition in plant fiber materials by capillary zone electrophoresis

The neutral sugar composition of acid hydrolyzed extracts of cellulose fiber samples, i.e. oat spelt, wheat straw, thermomechanica pulp (TMP) made of spruce, aspen stemwood, and bleached birch kraft pulp, was determined by a new capillary zone electrophoresis (CZE) method employing an alkaline background electrolyte. The method relies on in-capillary reaction and direct UV detection at wavelength 270 nm. Neutral carbohydrates d-(+)-galactose, d-(+)-glucose, l-rhamnose, d-(+)-mannose, d-(−)-arabinose, and d-(+)-xylose were simultaneously separated. The calibration plots were linear over a range from 10 to 150 mg/L for d-(+)-galactose, l-rhamnose, d-(+)-mannose, and d-(−)-arabinose and from 50 to 400 mg/L for d-(+)-glucose and d-(+)-xylose. Relative standard deviations (RSDs) of peak areas during a 5-day analysis period varied from 3.3% for galactose to 11.8% for rhamnose. RSDs of migration times varied between 0.3 and 0.7%. The detection limit (at S/N 3) was 5 mg/L for each monosaccharide. The results obtained by CZE agreed well with results obtained by high-performance anion-exchange chromatography. Glucose and xylose were the two predominant monosaccharides in the plants, except in the spruce TMP sample where glucose and mannose dominated.