Determination of neutral carbohydrates by CZE with direct UV detection

Stella Rovio, Jari Yli-Kauhaluoma, Heli Sirén (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

68 Citations (Scopus)

Abstract

A new CZE method relying on in-capillary reaction and direct UV detection at the wavelength 270 nm is presented for the simultaneous separation of the neutral carbohydrates xylitol, D-(-)-mannitol, sucrose, D-(+)-fucose, D-(+)-cellobiose, D-(+)-galactose, D-(+)-glucose, L-rhamnose, D-(+)-mannose, D-(-)-arabinose, D-(+)-xylose, and D-(-)-ribose. The alkaline electrolyte solution was prepared of 130 mM sodium hydroxide and 36 mM disodium hydrogen phosphate dihydrate. Separation of the sample mixture was achieved within 35 min. Calibration plots were linear in the range of 0.05-3 mM. Reproducibility of migration times was between 0.3 and 1.1%, and the detection limits for the analytes were 0.02 and 0.05 mM. The optimized method was applied for the determination of neutral monosaccharides in lemon, pineapple, and orange juices and a cognac sample. The methodology is fast since no other sample preparation except dilution is required.
Original languageEnglish
Article number3129–3135
JournalElectrophoresis
Volume28
Issue number17
DOIs
Publication statusPublished - 2007
MoE publication typeA1 Journal article-refereed

Fingerprint

Carbohydrates
Ananas
Xylitol
Cellobiose
Rhamnose
Sodium Hydroxide
Arabinose
Ribose
Fucose
Monosaccharides
Xylose
Mannitol
Mannose
Galactose
Electrolytes
Calibration
Dilution
Sucrose
Limit of Detection
Glucose

Keywords

  • CE
  • Direct UV detection
  • Neutral carbohydrate

Cite this

Rovio, Stella ; Yli-Kauhaluoma, Jari ; Sirén, Heli. / Determination of neutral carbohydrates by CZE with direct UV detection. In: Electrophoresis. 2007 ; Vol. 28, No. 17.
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Rovio, S, Yli-Kauhaluoma, J & Sirén, H 2007, 'Determination of neutral carbohydrates by CZE with direct UV detection', Electrophoresis, vol. 28, no. 17, 3129–3135. https://doi.org/10.1002/elps.200600783

Determination of neutral carbohydrates by CZE with direct UV detection. / Rovio, Stella; Yli-Kauhaluoma, Jari; Sirén, Heli (Corresponding Author).

In: Electrophoresis, Vol. 28, No. 17, 3129–3135, 2007.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Determination of neutral carbohydrates by CZE with direct UV detection

AU - Rovio, Stella

AU - Yli-Kauhaluoma, Jari

AU - Sirén, Heli

PY - 2007

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N2 - A new CZE method relying on in-capillary reaction and direct UV detection at the wavelength 270 nm is presented for the simultaneous separation of the neutral carbohydrates xylitol, D-(-)-mannitol, sucrose, D-(+)-fucose, D-(+)-cellobiose, D-(+)-galactose, D-(+)-glucose, L-rhamnose, D-(+)-mannose, D-(-)-arabinose, D-(+)-xylose, and D-(-)-ribose. The alkaline electrolyte solution was prepared of 130 mM sodium hydroxide and 36 mM disodium hydrogen phosphate dihydrate. Separation of the sample mixture was achieved within 35 min. Calibration plots were linear in the range of 0.05-3 mM. Reproducibility of migration times was between 0.3 and 1.1%, and the detection limits for the analytes were 0.02 and 0.05 mM. The optimized method was applied for the determination of neutral monosaccharides in lemon, pineapple, and orange juices and a cognac sample. The methodology is fast since no other sample preparation except dilution is required.

AB - A new CZE method relying on in-capillary reaction and direct UV detection at the wavelength 270 nm is presented for the simultaneous separation of the neutral carbohydrates xylitol, D-(-)-mannitol, sucrose, D-(+)-fucose, D-(+)-cellobiose, D-(+)-galactose, D-(+)-glucose, L-rhamnose, D-(+)-mannose, D-(-)-arabinose, D-(+)-xylose, and D-(-)-ribose. The alkaline electrolyte solution was prepared of 130 mM sodium hydroxide and 36 mM disodium hydrogen phosphate dihydrate. Separation of the sample mixture was achieved within 35 min. Calibration plots were linear in the range of 0.05-3 mM. Reproducibility of migration times was between 0.3 and 1.1%, and the detection limits for the analytes were 0.02 and 0.05 mM. The optimized method was applied for the determination of neutral monosaccharides in lemon, pineapple, and orange juices and a cognac sample. The methodology is fast since no other sample preparation except dilution is required.

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KW - Neutral carbohydrate

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SN - 0173-0835

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