Determination of taxol by high-performance liquid chromatography-thermospray mass spectrometry

Seppo Auriola; Anna-Maija Lepistö; Toivo Naaranlahti; Seppo Lapinjoki

doi:10.1016/0021-9673(92)80324-N

Determination of taxol by high-performance liquid chromatography-thermospray mass spectrometry

Seppo Auriola, Anna-Maija Lepistö, Toivo Naaranlahti, Seppo Lapinjoki

VTT Technical Research Centre of Finland

University of Eastern Finland

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Abstract

A high-performance liquid chromatography-thermospray mass spectrometry method has been developed for the determination of taxol, found in the Taxus species. The compound is chromatographed by isocratic elution in 14 min and is quantitated by selected-ion recording of the protonated molecule. The method is linear over the range 1–1000 ng (1.2 pmol-1.2 nmol) of taxol per injection. The standard deviation of replicate bark samples (n = 6) was 12.8%.

Original language	English
Pages (from-to)	153-158
Journal	Journal of Chromatography
Volume	594
Issue number	1-2
DOIs	https://doi.org/10.1016/0021-9673(92)80324-N
Publication status	Published - 1992
MoE publication type	A1 Journal article-refereed

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title = "Determination of taxol by high-performance liquid chromatography-thermospray mass spectrometry",

abstract = "A high-performance liquid chromatography-thermospray mass spectrometry method has been developed for the determination of taxol, found in the Taxus species. The compound is chromatographed by isocratic elution in 14 min and is quantitated by selected-ion recording of the protonated molecule. The method is linear over the range 1–1000 ng (1.2 pmol-1.2 nmol) of taxol per injection. The standard deviation of replicate bark samples (n = 6) was 12.8%.",

author = "Seppo Auriola and Anna-Maija Lepist{\"o} and Toivo Naaranlahti and Seppo Lapinjoki",

note = "Project code: BIO9001",

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AU - Auriola, Seppo

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AU - Naaranlahti, Toivo

AU - Lapinjoki, Seppo

N1 - Project code: BIO9001

PY - 1992

Y1 - 1992

N2 - A high-performance liquid chromatography-thermospray mass spectrometry method has been developed for the determination of taxol, found in the Taxus species. The compound is chromatographed by isocratic elution in 14 min and is quantitated by selected-ion recording of the protonated molecule. The method is linear over the range 1–1000 ng (1.2 pmol-1.2 nmol) of taxol per injection. The standard deviation of replicate bark samples (n = 6) was 12.8%.

AB - A high-performance liquid chromatography-thermospray mass spectrometry method has been developed for the determination of taxol, found in the Taxus species. The compound is chromatographed by isocratic elution in 14 min and is quantitated by selected-ion recording of the protonated molecule. The method is linear over the range 1–1000 ng (1.2 pmol-1.2 nmol) of taxol per injection. The standard deviation of replicate bark samples (n = 6) was 12.8%.

U2 - 10.1016/0021-9673(92)80324-N

DO - 10.1016/0021-9673(92)80324-N

M3 - Article

SN - 0021-9673

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SP - 153

EP - 158

JO - Journal of Chromatography

JF - Journal of Chromatography

IS - 1-2

ER -

Determination of taxol by high-performance liquid chromatography-thermospray mass spectrometry

Abstract

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