Determination of taxol by high-performance liquid chromatography-thermospray mass spectrometry

Seppo Auriola, Anna-Maija Lepistö, Toivo Naaranlahti, Seppo Lapinjoki

Research output: Contribution to journalArticleScientificpeer-review

52 Citations (Scopus)

Abstract

A high-performance liquid chromatography-thermospray mass spectrometry method has been developed for the determination of taxol, found in the Taxus species. The compound is chromatographed by isocratic elution in 14 min and is quantitated by selected-ion recording of the protonated molecule. The method is linear over the range 1–1000 ng (1.2 pmol-1.2 nmol) of taxol per injection. The standard deviation of replicate bark samples (n = 6) was 12.8%.
Original languageEnglish
Pages (from-to)153-158
Number of pages6
JournalJournal of Chromatography
Volume594
Issue number1-2
DOIs
Publication statusPublished - 1992
MoE publication typeA1 Journal article-refereed

Fingerprint

High performance liquid chromatography
Paclitaxel
Mass spectrometry
Mass Spectrometry
High Pressure Liquid Chromatography
Taxus
Ions
Injections
Molecules

Cite this

Auriola, Seppo ; Lepistö, Anna-Maija ; Naaranlahti, Toivo ; Lapinjoki, Seppo. / Determination of taxol by high-performance liquid chromatography-thermospray mass spectrometry. In: Journal of Chromatography. 1992 ; Vol. 594, No. 1-2. pp. 153-158.
@article{eff8e9ef77f149bc91b9d82ada0a8fcf,
title = "Determination of taxol by high-performance liquid chromatography-thermospray mass spectrometry",
abstract = "A high-performance liquid chromatography-thermospray mass spectrometry method has been developed for the determination of taxol, found in the Taxus species. The compound is chromatographed by isocratic elution in 14 min and is quantitated by selected-ion recording of the protonated molecule. The method is linear over the range 1–1000 ng (1.2 pmol-1.2 nmol) of taxol per injection. The standard deviation of replicate bark samples (n = 6) was 12.8{\%}.",
author = "Seppo Auriola and Anna-Maija Lepist{\"o} and Toivo Naaranlahti and Seppo Lapinjoki",
note = "Project code: BIO9001",
year = "1992",
doi = "10.1016/0021-9673(92)80324-N",
language = "English",
volume = "594",
pages = "153--158",
journal = "Journal of Chromatography A",
issn = "0021-9673",
publisher = "Elsevier",
number = "1-2",

}

Determination of taxol by high-performance liquid chromatography-thermospray mass spectrometry. / Auriola, Seppo; Lepistö, Anna-Maija; Naaranlahti, Toivo; Lapinjoki, Seppo.

In: Journal of Chromatography, Vol. 594, No. 1-2, 1992, p. 153-158.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Determination of taxol by high-performance liquid chromatography-thermospray mass spectrometry

AU - Auriola, Seppo

AU - Lepistö, Anna-Maija

AU - Naaranlahti, Toivo

AU - Lapinjoki, Seppo

N1 - Project code: BIO9001

PY - 1992

Y1 - 1992

N2 - A high-performance liquid chromatography-thermospray mass spectrometry method has been developed for the determination of taxol, found in the Taxus species. The compound is chromatographed by isocratic elution in 14 min and is quantitated by selected-ion recording of the protonated molecule. The method is linear over the range 1–1000 ng (1.2 pmol-1.2 nmol) of taxol per injection. The standard deviation of replicate bark samples (n = 6) was 12.8%.

AB - A high-performance liquid chromatography-thermospray mass spectrometry method has been developed for the determination of taxol, found in the Taxus species. The compound is chromatographed by isocratic elution in 14 min and is quantitated by selected-ion recording of the protonated molecule. The method is linear over the range 1–1000 ng (1.2 pmol-1.2 nmol) of taxol per injection. The standard deviation of replicate bark samples (n = 6) was 12.8%.

U2 - 10.1016/0021-9673(92)80324-N

DO - 10.1016/0021-9673(92)80324-N

M3 - Article

VL - 594

SP - 153

EP - 158

JO - Journal of Chromatography A

JF - Journal of Chromatography A

SN - 0021-9673

IS - 1-2

ER -