Abstract
Introduction: Rhazya stricta Decne. (Apocynaceae) is a
medicinal plant rich in terpenoid indole alkaloids
(TIAs), some of which possess important pharmacological
properties. The study material including transgenic hairy
root cultures have been developed and their potential for
alkaloid production are being investigated. Objective: In
this study, a comprehensive GC-MS method for qualitative
and quantitative analysis of alkaloids from Rhazya hairy
roots was developed. Methods: The composition of
alkaloids was determined by using GC-MS. In
quantification, the ratio between alkaloid and internal
standard was based on extracted ion from total ion
current (TIC) analyses. Results: The developed method was
validated. An acceptable precision with RSD?8% over a
linear range of 1 to 100?g/mL was achieved. The accuracy
of the method was within 94-107%. Analysis of hairy root
extracts indicated the occurrence of a total of 20 TIAs.
Six of them, pleiocarpamine, fluorocarpamine, vincamine,
ajmalicine and two yohimbine isomers are reported here
for the first time in Rhazya. Trimethylsilyl (TMS)
derivatisation of the extracts resulted in the separation
of two isomers for yohimbine and also for
vallesiachotamine. Clearly improved chromatographic
profiles of TMS-derivatives were observed for vincanine
and for minor compounds vincamine and rhazine.
Conclusion: The results show that the present GC-MS
method is reliable and well applicable for studying the
variation of indole alkaloids in Rhazya samples.
| Original language | English |
|---|---|
| Pages (from-to) | 331-338 |
| Journal | Phytochemical Analysis |
| Volume | 26 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2015 |
| MoE publication type | A1 Journal article-refereed |
Keywords
- Rhazya stricta
- transformed hairy roots
- terpenoid indole alkaloids
- GC-MS
- extracted ion monitoring
- trimethylsilyl derivatisation