Determination of the carboxylic acids in acidic and basic process samples by capillary zone electrophoresis

Stella Rovio, Anna Kalliola, Heli Sirén (Corresponding Author), Tarja Tamminen

Research output: Contribution to journalArticleScientificpeer-review

27 Citations (Scopus)

Abstract

Capillary zone electrophoresis (CZE) with indirect UV detection was used in developing a method for the simultaneous determination of inorganic anions, aliphatic and heterocyclic organic acids in various processed samples. The analytes were determined simultaneously in 10 min using an electrolyte containing 20 mM 2,3-pyrazine dicarboxylic acid, 65 mM tricine, 2 mM BaCl2, 0.5 mM cetyltrimethylammonium bromide, and 2 M urea at pH 8.06. Linear plots for the analytes were obtained in the concentration range of 2–150 mg L−1. Relative standard deviations (RSDs) of peak areas during a 3-day analysis period varied from 5.5% for glycolate to 9.5% for oxalate. RSDs of migration times varied between 0.4% and 1.1%. The detection limit (at S/N 3) was 1 mg L−1 for all the analytes studied. The proposed method was successfully demonstrated for the determination of carboxylic acids in eight oxygen treated samples of commercial softwood and hardwood kraft lignin and two red wine samples of Pinot Noir grapes. In the kraft lignin samples the concentrations of carboxylic acids correspond to the oxidation time. The acid concentrations of wine varied considerable.
Original languageEnglish
Pages (from-to)1407-1413
Number of pages7
JournalJournal of Chromatography A
Volume1217
Issue number8
DOIs
Publication statusPublished - 2010
MoE publication typeA1 Journal article-refereed

Fingerprint

Wine
glycolic acid
Capillary Electrophoresis
Carboxylic Acids
Electrophoresis
Heterocyclic Acids
Pyrazines
Dicarboxylic Acids
Oxalates
Softwoods
Hardwoods
Organic acids
Vitis
Electrolytes
Anions
Urea
Limit of Detection
Demography
Oxygen
Oxidation

Keywords

  • Capillary electrophoresis
  • Carboxylic acid
  • Indirect UV detection
  • Wine
  • Lignin

Cite this

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title = "Determination of the carboxylic acids in acidic and basic process samples by capillary zone electrophoresis",
abstract = "Capillary zone electrophoresis (CZE) with indirect UV detection was used in developing a method for the simultaneous determination of inorganic anions, aliphatic and heterocyclic organic acids in various processed samples. The analytes were determined simultaneously in 10 min using an electrolyte containing 20 mM 2,3-pyrazine dicarboxylic acid, 65 mM tricine, 2 mM BaCl2, 0.5 mM cetyltrimethylammonium bromide, and 2 M urea at pH 8.06. Linear plots for the analytes were obtained in the concentration range of 2–150 mg L−1. Relative standard deviations (RSDs) of peak areas during a 3-day analysis period varied from 5.5{\%} for glycolate to 9.5{\%} for oxalate. RSDs of migration times varied between 0.4{\%} and 1.1{\%}. The detection limit (at S/N 3) was 1 mg L−1 for all the analytes studied. The proposed method was successfully demonstrated for the determination of carboxylic acids in eight oxygen treated samples of commercial softwood and hardwood kraft lignin and two red wine samples of Pinot Noir grapes. In the kraft lignin samples the concentrations of carboxylic acids correspond to the oxidation time. The acid concentrations of wine varied considerable.",
keywords = "Capillary electrophoresis, Carboxylic acid, Indirect UV detection, Wine, Lignin",
author = "Stella Rovio and Anna Kalliola and Heli Sir{\'e}n and Tarja Tamminen",
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language = "English",
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Determination of the carboxylic acids in acidic and basic process samples by capillary zone electrophoresis. / Rovio, Stella; Kalliola, Anna; Sirén, Heli (Corresponding Author); Tamminen, Tarja.

In: Journal of Chromatography A, Vol. 1217, No. 8, 2010, p. 1407-1413.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Determination of the carboxylic acids in acidic and basic process samples by capillary zone electrophoresis

AU - Rovio, Stella

AU - Kalliola, Anna

AU - Sirén, Heli

AU - Tamminen, Tarja

N1 - Project code: 38778

PY - 2010

Y1 - 2010

N2 - Capillary zone electrophoresis (CZE) with indirect UV detection was used in developing a method for the simultaneous determination of inorganic anions, aliphatic and heterocyclic organic acids in various processed samples. The analytes were determined simultaneously in 10 min using an electrolyte containing 20 mM 2,3-pyrazine dicarboxylic acid, 65 mM tricine, 2 mM BaCl2, 0.5 mM cetyltrimethylammonium bromide, and 2 M urea at pH 8.06. Linear plots for the analytes were obtained in the concentration range of 2–150 mg L−1. Relative standard deviations (RSDs) of peak areas during a 3-day analysis period varied from 5.5% for glycolate to 9.5% for oxalate. RSDs of migration times varied between 0.4% and 1.1%. The detection limit (at S/N 3) was 1 mg L−1 for all the analytes studied. The proposed method was successfully demonstrated for the determination of carboxylic acids in eight oxygen treated samples of commercial softwood and hardwood kraft lignin and two red wine samples of Pinot Noir grapes. In the kraft lignin samples the concentrations of carboxylic acids correspond to the oxidation time. The acid concentrations of wine varied considerable.

AB - Capillary zone electrophoresis (CZE) with indirect UV detection was used in developing a method for the simultaneous determination of inorganic anions, aliphatic and heterocyclic organic acids in various processed samples. The analytes were determined simultaneously in 10 min using an electrolyte containing 20 mM 2,3-pyrazine dicarboxylic acid, 65 mM tricine, 2 mM BaCl2, 0.5 mM cetyltrimethylammonium bromide, and 2 M urea at pH 8.06. Linear plots for the analytes were obtained in the concentration range of 2–150 mg L−1. Relative standard deviations (RSDs) of peak areas during a 3-day analysis period varied from 5.5% for glycolate to 9.5% for oxalate. RSDs of migration times varied between 0.4% and 1.1%. The detection limit (at S/N 3) was 1 mg L−1 for all the analytes studied. The proposed method was successfully demonstrated for the determination of carboxylic acids in eight oxygen treated samples of commercial softwood and hardwood kraft lignin and two red wine samples of Pinot Noir grapes. In the kraft lignin samples the concentrations of carboxylic acids correspond to the oxidation time. The acid concentrations of wine varied considerable.

KW - Capillary electrophoresis

KW - Carboxylic acid

KW - Indirect UV detection

KW - Wine

KW - Lignin

U2 - 10.1016/j.chroma.2009.12.032

DO - 10.1016/j.chroma.2009.12.032

M3 - Article

VL - 1217

SP - 1407

EP - 1413

JO - Journal of Chromatography A

JF - Journal of Chromatography A

SN - 0021-9673

IS - 8

ER -