Determination of the main tropane alkaloids from transformed Hyoscyamus muticus plants by capillary zone electrophoresis

Manu Eeva, Jukka Pekka Salo, Kirsi Marja Oksman-Caldentey

Research output: Contribution to journalArticleScientificpeer-review

42 Citations (Scopus)

Abstract

A capillary zone electrophoretic method (CZE) was developed using an uncoated fused silica capillary for the separation and determination of the main tropane alkaloids. The applicability of the developed method for analysis of plant samples was examined by analyzing samples of transgenic Egyptian henbane Hyoscamus muticus (L.) plants. A simple 40 mM phosphate buffer at pH 7.8 using a voltage of 20 kV was found the best for this purpose. The main tropane alkaloids, atropine and scopolamine as well as nor-(-)-scopolamine, and tropic acid, the precursor of tropane alkaloids, could be separated in less than 13 min. The linear concentration range for atropine was 5.00-140 μg ml-1, for scopolamine 7.50-210 μg ml-1 and for tropic acid 2.50-70.0 μg ml-1.

Original languageEnglish
Pages (from-to)717-722
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume16
Issue number5
DOIs
Publication statusPublished - 1 Jan 1998
MoE publication typeA1 Journal article-refereed

Fingerprint

Hyoscyamus
Tropanes
Scopolamine Hydrobromide
Capillary Electrophoresis
Electrophoresis
Alkaloids
Atropine
Fused silica
Silicon Dioxide
Buffers
Phosphates
Electric potential
tropic acid

Keywords

  • Capillary zone electrophoresis
  • Transgenic plants
  • Tropane alkaloids

Cite this

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abstract = "A capillary zone electrophoretic method (CZE) was developed using an uncoated fused silica capillary for the separation and determination of the main tropane alkaloids. The applicability of the developed method for analysis of plant samples was examined by analyzing samples of transgenic Egyptian henbane Hyoscamus muticus (L.) plants. A simple 40 mM phosphate buffer at pH 7.8 using a voltage of 20 kV was found the best for this purpose. The main tropane alkaloids, atropine and scopolamine as well as nor-(-)-scopolamine, and tropic acid, the precursor of tropane alkaloids, could be separated in less than 13 min. The linear concentration range for atropine was 5.00-140 μg ml-1, for scopolamine 7.50-210 μg ml-1 and for tropic acid 2.50-70.0 μg ml-1.",
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Determination of the main tropane alkaloids from transformed Hyoscyamus muticus plants by capillary zone electrophoresis. / Eeva, Manu; Salo, Jukka Pekka; Oksman-Caldentey, Kirsi Marja.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 16, No. 5, 01.01.1998, p. 717-722.

Research output: Contribution to journalArticleScientificpeer-review

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