Determination of urinary catecholamines with capillary electrophoresis after solid-phase extraction

Katariina Vuorensola, Heli Siren (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

52 Citations (Scopus)

Abstract

The stabilities of 3,4-dihydroxybenzylamine (DHBA), dopamine, 3-methoxytyramine, normetanephrine and metanephrine standards under acid, base and enzymatic hydrolysis conditions were studied. Basic incubation media were not suitable for 3,4-dihydroxy compounds, but acid and enzymatic hydrolysis conditions were applicable to all the compounds. The results of acid and enzymatic hydrolysis were comparable and the enzymatic hydrolysis was applied to a urine matrix. A method including solid-phase extraction (SPE) with a copolymer sorbent was developed for purification of the urine samples. Due to poor recovery of DHBA, the most frequently used internal standard in catecholamine analysis, this compound was replaced with the 3-O-methoxy structure. The recoveries of the compounds in spiked urine samples in SPE were between 96.4 and 124.4%. The repeatability of the combination of enzymatic hydrolysis and SPE pretreatment was good for all the compounds, except for dopamine and 3-methoxytyramine due to some matrix compounds still interfering with the separation. The analyses were performed with capillary electrophoresis in an ammonium acetate buffer with UV detection. The validation data for the compounds including limit of detection, limit of quantification, linearity and repeatability of the method are presented.

Original languageEnglish
Pages (from-to)317 - 327
Number of pages11
JournalJournal of Chromatography A
Volume895
Issue number1-2
DOIs
Publication statusPublished - 2000
MoE publication typeA1 Journal article-refereed

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Capillary electrophoresis
Enzymatic hydrolysis
Solid Phase Extraction
Capillary Electrophoresis
Catecholamines
Hydrolysis
Urine
Acids
Dopamine
Normetanephrine
Metanephrine
Recovery
Sorbents
Purification
Limit of Detection
Buffers
Copolymers

Cite this

Vuorensola, Katariina ; Siren, Heli. / Determination of urinary catecholamines with capillary electrophoresis after solid-phase extraction. In: Journal of Chromatography A. 2000 ; Vol. 895, No. 1-2. pp. 317 - 327.
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Determination of urinary catecholamines with capillary electrophoresis after solid-phase extraction. / Vuorensola, Katariina; Siren, Heli (Corresponding Author).

In: Journal of Chromatography A, Vol. 895, No. 1-2, 2000, p. 317 - 327.

Research output: Contribution to journalArticleScientificpeer-review

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AB - The stabilities of 3,4-dihydroxybenzylamine (DHBA), dopamine, 3-methoxytyramine, normetanephrine and metanephrine standards under acid, base and enzymatic hydrolysis conditions were studied. Basic incubation media were not suitable for 3,4-dihydroxy compounds, but acid and enzymatic hydrolysis conditions were applicable to all the compounds. The results of acid and enzymatic hydrolysis were comparable and the enzymatic hydrolysis was applied to a urine matrix. A method including solid-phase extraction (SPE) with a copolymer sorbent was developed for purification of the urine samples. Due to poor recovery of DHBA, the most frequently used internal standard in catecholamine analysis, this compound was replaced with the 3-O-methoxy structure. The recoveries of the compounds in spiked urine samples in SPE were between 96.4 and 124.4%. The repeatability of the combination of enzymatic hydrolysis and SPE pretreatment was good for all the compounds, except for dopamine and 3-methoxytyramine due to some matrix compounds still interfering with the separation. The analyses were performed with capillary electrophoresis in an ammonium acetate buffer with UV detection. The validation data for the compounds including limit of detection, limit of quantification, linearity and repeatability of the method are presented.

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