Determination of xylose and glucose in a flow-injection system with PQQ-dependent aldose dehydrogenase

Maria Smolander; Julia Cooper; Wolfgang Schuhmann; Martin Hämmerle; Hanns-Ludwig Schmidt

doi:10.1016/0003-2670(93)80249-K

Determination of xylose and glucose in a flow-injection system with PQQ-dependent aldose dehydrogenase

Maria Smolander (Corresponding Author), Julia Cooper, Wolfgang Schuhmann, Martin Hämmerle, Hanns-Ludwig Schmidt

Technical University of Munich (TUM)

Research output: Contribution to journal › Article › Scientific › peer-review

25 Citations (Scopus)

Abstract

Application of pyrroloquinoline quinone (PQQ)-dependant aldose dehydrogenase (ALDH) for the amperometric determination of xylose and glucose is described. Different flow injection configurations were investigated to optimize the measurement. The highest current densities could be obtained when the enzyme was immobilized directly on a graphite electrode. The best stability was achieved with controlled-pore glass (CPG)-immobilized ALDH. ALDH-CPG was also successfully used for the measurement of real fermentation samples. The measurement of samples containing only xylose as a carbon source correlated very well with high-pressure liquid chromatographic measurement, the correlation coefficient and conversion factor between the methods being 0.98 and 0.96, respectively. Also samples containing both xylose and glucose could be measured satisfactorily when the measurement with ALDH was combined with separate glucose measurement.

Original language	English
Pages (from-to)	119 - 127
Number of pages	9
Journal	Analytica Chimica Acta
Volume	280
Issue number	1
DOIs	https://doi.org/10.1016/0003-2670(93)80249-K
Publication status	Published - 1993
MoE publication type	A1 Journal article-refereed

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title = "Determination of xylose and glucose in a flow-injection system with PQQ-dependent aldose dehydrogenase",

abstract = "Application of pyrroloquinoline quinone (PQQ)-dependant aldose dehydrogenase (ALDH) for the amperometric determination of xylose and glucose is described. Different flow injection configurations were investigated to optimize the measurement. The highest current densities could be obtained when the enzyme was immobilized directly on a graphite electrode. The best stability was achieved with controlled-pore glass (CPG)-immobilized ALDH. ALDH-CPG was also successfully used for the measurement of real fermentation samples. The measurement of samples containing only xylose as a carbon source correlated very well with high-pressure liquid chromatographic measurement, the correlation coefficient and conversion factor between the methods being 0.98 and 0.96, respectively. Also samples containing both xylose and glucose could be measured satisfactorily when the measurement with ALDH was combined with separate glucose measurement.",

author = "Maria Smolander and Julia Cooper and Wolfgang Schuhmann and Martin H{\"a}mmerle and Hanns-Ludwig Schmidt",

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T1 - Determination of xylose and glucose in a flow-injection system with PQQ-dependent aldose dehydrogenase

AU - Smolander, Maria

AU - Cooper, Julia

AU - Schuhmann, Wolfgang

AU - Hämmerle, Martin

AU - Schmidt, Hanns-Ludwig

N1 - Project code: -

PY - 1993

Y1 - 1993

N2 - Application of pyrroloquinoline quinone (PQQ)-dependant aldose dehydrogenase (ALDH) for the amperometric determination of xylose and glucose is described. Different flow injection configurations were investigated to optimize the measurement. The highest current densities could be obtained when the enzyme was immobilized directly on a graphite electrode. The best stability was achieved with controlled-pore glass (CPG)-immobilized ALDH. ALDH-CPG was also successfully used for the measurement of real fermentation samples. The measurement of samples containing only xylose as a carbon source correlated very well with high-pressure liquid chromatographic measurement, the correlation coefficient and conversion factor between the methods being 0.98 and 0.96, respectively. Also samples containing both xylose and glucose could be measured satisfactorily when the measurement with ALDH was combined with separate glucose measurement.

AB - Application of pyrroloquinoline quinone (PQQ)-dependant aldose dehydrogenase (ALDH) for the amperometric determination of xylose and glucose is described. Different flow injection configurations were investigated to optimize the measurement. The highest current densities could be obtained when the enzyme was immobilized directly on a graphite electrode. The best stability was achieved with controlled-pore glass (CPG)-immobilized ALDH. ALDH-CPG was also successfully used for the measurement of real fermentation samples. The measurement of samples containing only xylose as a carbon source correlated very well with high-pressure liquid chromatographic measurement, the correlation coefficient and conversion factor between the methods being 0.98 and 0.96, respectively. Also samples containing both xylose and glucose could be measured satisfactorily when the measurement with ALDH was combined with separate glucose measurement.

U2 - 10.1016/0003-2670(93)80249-K

DO - 10.1016/0003-2670(93)80249-K

M3 - Article

SN - 0003-2670

VL - 280

SP - 119

EP - 127

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

IS - 1

ER -

Determination of xylose and glucose in a flow-injection system with PQQ-dependent aldose dehydrogenase

Abstract

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