Determination of xylose and glucose in a flow-injection system with PQQ-dependent aldose dehydrogenase

Maria Smolander (Corresponding Author), Julia Cooper, Wolfgang Schuhmann, Martin Hämmerle, Hanns-Ludwig Schmidt

Research output: Contribution to journalArticleScientificpeer-review

23 Citations (Scopus)

Abstract

Application of pyrroloquinoline quinone (PQQ)-dependant aldose dehydrogenase (ALDH) for the amperometric determination of xylose and glucose is described. Different flow injection configurations were investigated to optimize the measurement. The highest current densities could be obtained when the enzyme was immobilized directly on a graphite electrode. The best stability was achieved with controlled-pore glass (CPG)-immobilized ALDH. ALDH-CPG was also successfully used for the measurement of real fermentation samples. The measurement of samples containing only xylose as a carbon source correlated very well with high-pressure liquid chromatographic measurement, the correlation coefficient and conversion factor between the methods being 0.98 and 0.96, respectively. Also samples containing both xylose and glucose could be measured satisfactorily when the measurement with ALDH was combined with separate glucose measurement.

Original languageEnglish
Pages (from-to)119 - 127
Number of pages9
JournalAnalytica Chimica Acta
Volume280
Issue number1
DOIs
Publication statusPublished - 1993
MoE publication typeA1 Journal article-refereed

Fingerprint

PQQ Cofactor
Oxidoreductases
glucose
Injections
Glass
glass
Immobilized Enzymes
Graphite
Xylose
density current
graphite
Fermentation
fermentation
Electrodes
electrode
Carbon
enzyme
Pressure
Glucose
Graphite electrodes

Cite this

Smolander, Maria ; Cooper, Julia ; Schuhmann, Wolfgang ; Hämmerle, Martin ; Schmidt, Hanns-Ludwig. / Determination of xylose and glucose in a flow-injection system with PQQ-dependent aldose dehydrogenase. In: Analytica Chimica Acta. 1993 ; Vol. 280, No. 1. pp. 119 - 127.
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abstract = "Application of pyrroloquinoline quinone (PQQ)-dependant aldose dehydrogenase (ALDH) for the amperometric determination of xylose and glucose is described. Different flow injection configurations were investigated to optimize the measurement. The highest current densities could be obtained when the enzyme was immobilized directly on a graphite electrode. The best stability was achieved with controlled-pore glass (CPG)-immobilized ALDH. ALDH-CPG was also successfully used for the measurement of real fermentation samples. The measurement of samples containing only xylose as a carbon source correlated very well with high-pressure liquid chromatographic measurement, the correlation coefficient and conversion factor between the methods being 0.98 and 0.96, respectively. Also samples containing both xylose and glucose could be measured satisfactorily when the measurement with ALDH was combined with separate glucose measurement.",
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Determination of xylose and glucose in a flow-injection system with PQQ-dependent aldose dehydrogenase. / Smolander, Maria (Corresponding Author); Cooper, Julia; Schuhmann, Wolfgang; Hämmerle, Martin; Schmidt, Hanns-Ludwig.

In: Analytica Chimica Acta, Vol. 280, No. 1, 1993, p. 119 - 127.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Determination of xylose and glucose in a flow-injection system with PQQ-dependent aldose dehydrogenase

AU - Smolander, Maria

AU - Cooper, Julia

AU - Schuhmann, Wolfgang

AU - Hämmerle, Martin

AU - Schmidt, Hanns-Ludwig

N1 - Project code: -

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AB - Application of pyrroloquinoline quinone (PQQ)-dependant aldose dehydrogenase (ALDH) for the amperometric determination of xylose and glucose is described. Different flow injection configurations were investigated to optimize the measurement. The highest current densities could be obtained when the enzyme was immobilized directly on a graphite electrode. The best stability was achieved with controlled-pore glass (CPG)-immobilized ALDH. ALDH-CPG was also successfully used for the measurement of real fermentation samples. The measurement of samples containing only xylose as a carbon source correlated very well with high-pressure liquid chromatographic measurement, the correlation coefficient and conversion factor between the methods being 0.98 and 0.96, respectively. Also samples containing both xylose and glucose could be measured satisfactorily when the measurement with ALDH was combined with separate glucose measurement.

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