Development of a high-throughput format for solid-phase extraction of enantiomers using an immunosorbent in 384-well plates

Tarja Nevanen (Corresponding Author), Helena Simolin, Tapani Suortti, Anu Koivula, Hans Söderlund

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

An antibody-based solid-phase extraction method for filtered 384-well plates was developed for a medical drug candidate having two enantiomeric forms in order to demonstrate the potential of the use of recombinant antibody fragments as specific and efficient immunosorbents. An immobilization method using a six-histidine tag of the antibody fragment and mild oxidation was applied in order to immobilize antibody fragments in an oriented and kinetically stable way that ensured high capacity of the antibody support. Phosphate buffer or plasma spiked with enantiomers were used as samples. Selective solid-phase extraction was followed by liquid chromatography−mass spectrometry analysis. Average recoveries for buffer and plasma samples ranged from 79 to 122% and 80 to 108%, respectively. Good linearity was observed in the concentration range of 30−3000 ng/mL of the enantiomer.
Original languageEnglish
Pages (from-to)3038 - 3044
Number of pages7
JournalAnalytical Chemistry
Volume77
Issue number10
DOIs
Publication statusPublished - 2005
MoE publication typeA1 Journal article-refereed

Fingerprint

Immunosorbents
Immunoglobulin Fragments
Enantiomers
Throughput
Buffers
Plasmas
Antibodies
Histidine
Spectrometry
Phosphates
Recovery
Oxidation
Liquids
Pharmaceutical Preparations

Keywords

  • antibodies
  • antibody fragments
  • solid-phase extraction
  • drug candidates
  • pharmaceutical compounds

Cite this

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abstract = "An antibody-based solid-phase extraction method for filtered 384-well plates was developed for a medical drug candidate having two enantiomeric forms in order to demonstrate the potential of the use of recombinant antibody fragments as specific and efficient immunosorbents. An immobilization method using a six-histidine tag of the antibody fragment and mild oxidation was applied in order to immobilize antibody fragments in an oriented and kinetically stable way that ensured high capacity of the antibody support. Phosphate buffer or plasma spiked with enantiomers were used as samples. Selective solid-phase extraction was followed by liquid chromatography−mass spectrometry analysis. Average recoveries for buffer and plasma samples ranged from 79 to 122{\%} and 80 to 108{\%}, respectively. Good linearity was observed in the concentration range of 30−3000 ng/mL of the enantiomer.",
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Development of a high-throughput format for solid-phase extraction of enantiomers using an immunosorbent in 384-well plates. / Nevanen, Tarja (Corresponding Author); Simolin, Helena; Suortti, Tapani; Koivula, Anu; Söderlund, Hans.

In: Analytical Chemistry, Vol. 77, No. 10, 2005, p. 3038 - 3044.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Development of a high-throughput format for solid-phase extraction of enantiomers using an immunosorbent in 384-well plates

AU - Nevanen, Tarja

AU - Simolin, Helena

AU - Suortti, Tapani

AU - Koivula, Anu

AU - Söderlund, Hans

PY - 2005

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AB - An antibody-based solid-phase extraction method for filtered 384-well plates was developed for a medical drug candidate having two enantiomeric forms in order to demonstrate the potential of the use of recombinant antibody fragments as specific and efficient immunosorbents. An immobilization method using a six-histidine tag of the antibody fragment and mild oxidation was applied in order to immobilize antibody fragments in an oriented and kinetically stable way that ensured high capacity of the antibody support. Phosphate buffer or plasma spiked with enantiomers were used as samples. Selective solid-phase extraction was followed by liquid chromatography−mass spectrometry analysis. Average recoveries for buffer and plasma samples ranged from 79 to 122% and 80 to 108%, respectively. Good linearity was observed in the concentration range of 30−3000 ng/mL of the enantiomer.

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KW - antibody fragments

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KW - drug candidates

KW - pharmaceutical compounds

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