Development of a low-cost cellulase production process using Trichoderma reesei for Brazilian biorefineries

Simo Ellilä (Corresponding Author), Lucas Fonseca, Cristiane Uchima, Junio Cota, Gustafo Henrique Goldman, Markku Saloheimo, Vera Sacon, Matti Siika-Aho

Research output: Contribution to journalArticleScientificpeer-review

27 Citations (Scopus)

Abstract

Background: During the past few years, the first industrial-scale cellulosic ethanol plants have been inaugurated. Although the performance of the commercial cellulase enzymes used in this process has greatly improved over the past decade, cellulases still represent a very significant operational cost. Depending on the region, transport of cellulases from a central production facility to a biorefinery may significantly add to enzyme cost. The aim of the present study was to develop a simple, cost-efficient cellulase production process that could be employed locally at a Brazilian sugarcane biorefinery. 
Results: Our work focused on two main topics: growth medium formulation and strain improvement. We evaluated several Brazilian low-cost industrial residues for their potential in cellulase production. Among the solid residues evaluated, soybean hulls were found to display clearly the most desirable characteristics. We engineered a Trichoderma reesei strain to secrete cellulase in the presence of repressing sugars, enabling the use of sugarcane molasses as an additional carbon source. In addition, we added a heterologous ß-glucosidase to improve the performance of the produced enzymes in hydrolysis. Finally, the addition of an invertase gene from Aspegillus niger into our strain allowed it to consume sucrose from sugarcane molasses directly. Preliminary cost analysis showed that the overall process can provide for very low-cost enzyme with good hydrolysis performance on industrially pre-treated sugarcane straw.
Conclusions: In this study, we showed that with relatively few genetic modifications and the right growth medium it is possible to produce considerable amounts of well-performing cellulase at very low cost in Brazil using T. reesei. With further enhancements and optimization, such a system could provide a viable alternative to delivered commercial cellulases.
Original languageEnglish
Pages (from-to)30
JournalBiotechnology for Biofuels
Volume10
Issue number1
DOIs
Publication statusPublished - 2017
MoE publication typeA1 Journal article-refereed

Fingerprint

Trichoderma
Cellulase
production cost
Saccharum
Costs and Cost Analysis
Cellulases
cost
enzyme
Enzymes
Costs
Molasses
Sugar (sucrose)
hydrolysis
Hydrolysis
Cellulosic ethanol
Glucosidases
cost analysis
beta-Fructofuranosidase
hull
sucrose

Keywords

  • on-site
  • cellulase
  • enzyme
  • Trichoderma reesei
  • sugarcane
  • molasses
  • Soybean hulls
  • Brazil
  • biorefinery
  • cellulosic ethanol

Cite this

Ellilä, Simo ; Fonseca, Lucas ; Uchima, Cristiane ; Cota, Junio ; Goldman, Gustafo Henrique ; Saloheimo, Markku ; Sacon, Vera ; Siika-Aho, Matti. / Development of a low-cost cellulase production process using Trichoderma reesei for Brazilian biorefineries. In: Biotechnology for Biofuels. 2017 ; Vol. 10, No. 1. pp. 30.
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Development of a low-cost cellulase production process using Trichoderma reesei for Brazilian biorefineries. / Ellilä, Simo (Corresponding Author); Fonseca, Lucas; Uchima, Cristiane; Cota, Junio; Goldman, Gustafo Henrique; Saloheimo, Markku; Sacon, Vera; Siika-Aho, Matti.

In: Biotechnology for Biofuels, Vol. 10, No. 1, 2017, p. 30.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Development of a low-cost cellulase production process using Trichoderma reesei for Brazilian biorefineries

AU - Ellilä, Simo

AU - Fonseca, Lucas

AU - Uchima, Cristiane

AU - Cota, Junio

AU - Goldman, Gustafo Henrique

AU - Saloheimo, Markku

AU - Sacon, Vera

AU - Siika-Aho, Matti

N1 - VTT Brasil Ltd.

PY - 2017

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N2 - Background: During the past few years, the first industrial-scale cellulosic ethanol plants have been inaugurated. Although the performance of the commercial cellulase enzymes used in this process has greatly improved over the past decade, cellulases still represent a very significant operational cost. Depending on the region, transport of cellulases from a central production facility to a biorefinery may significantly add to enzyme cost. The aim of the present study was to develop a simple, cost-efficient cellulase production process that could be employed locally at a Brazilian sugarcane biorefinery. Results: Our work focused on two main topics: growth medium formulation and strain improvement. We evaluated several Brazilian low-cost industrial residues for their potential in cellulase production. Among the solid residues evaluated, soybean hulls were found to display clearly the most desirable characteristics. We engineered a Trichoderma reesei strain to secrete cellulase in the presence of repressing sugars, enabling the use of sugarcane molasses as an additional carbon source. In addition, we added a heterologous ß-glucosidase to improve the performance of the produced enzymes in hydrolysis. Finally, the addition of an invertase gene from Aspegillus niger into our strain allowed it to consume sucrose from sugarcane molasses directly. Preliminary cost analysis showed that the overall process can provide for very low-cost enzyme with good hydrolysis performance on industrially pre-treated sugarcane straw.Conclusions: In this study, we showed that with relatively few genetic modifications and the right growth medium it is possible to produce considerable amounts of well-performing cellulase at very low cost in Brazil using T. reesei. With further enhancements and optimization, such a system could provide a viable alternative to delivered commercial cellulases.

AB - Background: During the past few years, the first industrial-scale cellulosic ethanol plants have been inaugurated. Although the performance of the commercial cellulase enzymes used in this process has greatly improved over the past decade, cellulases still represent a very significant operational cost. Depending on the region, transport of cellulases from a central production facility to a biorefinery may significantly add to enzyme cost. The aim of the present study was to develop a simple, cost-efficient cellulase production process that could be employed locally at a Brazilian sugarcane biorefinery. Results: Our work focused on two main topics: growth medium formulation and strain improvement. We evaluated several Brazilian low-cost industrial residues for their potential in cellulase production. Among the solid residues evaluated, soybean hulls were found to display clearly the most desirable characteristics. We engineered a Trichoderma reesei strain to secrete cellulase in the presence of repressing sugars, enabling the use of sugarcane molasses as an additional carbon source. In addition, we added a heterologous ß-glucosidase to improve the performance of the produced enzymes in hydrolysis. Finally, the addition of an invertase gene from Aspegillus niger into our strain allowed it to consume sucrose from sugarcane molasses directly. Preliminary cost analysis showed that the overall process can provide for very low-cost enzyme with good hydrolysis performance on industrially pre-treated sugarcane straw.Conclusions: In this study, we showed that with relatively few genetic modifications and the right growth medium it is possible to produce considerable amounts of well-performing cellulase at very low cost in Brazil using T. reesei. With further enhancements and optimization, such a system could provide a viable alternative to delivered commercial cellulases.

KW - on-site

KW - cellulase

KW - enzyme

KW - Trichoderma reesei

KW - sugarcane

KW - molasses

KW - Soybean hulls

KW - Brazil

KW - biorefinery

KW - cellulosic ethanol

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U2 - 10.1186/s13068-017-0717-0

DO - 10.1186/s13068-017-0717-0

M3 - Article

VL - 10

SP - 30

JO - Biotechnology for Biofuels

JF - Biotechnology for Biofuels

SN - 1754-6834

IS - 1

ER -