Development of a printable laccase-based biocathode for fuel cell applications

Maria Smolander (Corresponding Author), Harry Boer, Matti Valkiainen, Robert Roozeman, Mikael Bergelin, Jan-Erik Eriksson, Xia-Chang Zhang, Anu Koivula, Liisa Viikari

Research output: Contribution to journalArticleScientificpeer-review

69 Citations (Scopus)

Abstract

Laccases belong to the family of blue multicopper oxidases, which catalyze the four-electron reduction of dioxygen to water concomitantly through the oxidation of phenolic and other aromatic compounds. They are potential enzymes in many applications including biofuel cells to produce electricity through chemical reactions. We have tested here the incorporation of a high redox potential laccase from Trametes hirsuta in different types of conducting inks to produce dry printed enzyme electrode layers. ABTS was used as the redox mediator to shuttle the electrons between the surface of the cathodic electrode and the enzyme active sites. Our results demonstrate that the dry printed layers maintained their enzymatic activity even after several months. Furthermore, fuel cell prototypes could be constructed utilising an optimized printed laccase–ABTS layer as the cathode, and printed Zn layer as the anode. Under humidity controlled conditions, a cell voltage between 0.8 and 0.6 V could be maintained for several days under a 2.2 kΩ load. In addition, a corresponding stand-alone cell could be constructed where the cell voltage was maintained for 15 h under a load. These results offer a good starting point for further development of mass-producible, completely enzymatic printed biofuel cells.
Original languageEnglish
Pages (from-to)93 - 102
Number of pages10
JournalEnzyme and Microbial Technology
Volume43
Issue number2
DOIs
Publication statusPublished - 2008
MoE publication typeA1 Journal article-refereed

Fingerprint

Biological fuel cells
Laccase
Bioelectric Energy Sources
Fuel cells
Enzymes
Biosensing Techniques
Enzyme electrodes
Oxidation-Reduction
Electrons
Aromatic compounds
Electrodes
Electric potential
Ink
Trametes
Chemical reactions
Atmospheric humidity
Electricity
Oxidoreductases
Anodes
Cathodes

Keywords

  • biofuel cell
  • printing
  • enzyme
  • oxidoreductase
  • mediator
  • ink
  • carbon nanotube

Cite this

Smolander, Maria ; Boer, Harry ; Valkiainen, Matti ; Roozeman, Robert ; Bergelin, Mikael ; Eriksson, Jan-Erik ; Zhang, Xia-Chang ; Koivula, Anu ; Viikari, Liisa. / Development of a printable laccase-based biocathode for fuel cell applications. In: Enzyme and Microbial Technology. 2008 ; Vol. 43, No. 2. pp. 93 - 102.
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Development of a printable laccase-based biocathode for fuel cell applications. / Smolander, Maria (Corresponding Author); Boer, Harry; Valkiainen, Matti; Roozeman, Robert; Bergelin, Mikael; Eriksson, Jan-Erik; Zhang, Xia-Chang; Koivula, Anu; Viikari, Liisa.

In: Enzyme and Microbial Technology, Vol. 43, No. 2, 2008, p. 93 - 102.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Smolander, Maria

AU - Boer, Harry

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AU - Roozeman, Robert

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AU - Eriksson, Jan-Erik

AU - Zhang, Xia-Chang

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AB - Laccases belong to the family of blue multicopper oxidases, which catalyze the four-electron reduction of dioxygen to water concomitantly through the oxidation of phenolic and other aromatic compounds. They are potential enzymes in many applications including biofuel cells to produce electricity through chemical reactions. We have tested here the incorporation of a high redox potential laccase from Trametes hirsuta in different types of conducting inks to produce dry printed enzyme electrode layers. ABTS was used as the redox mediator to shuttle the electrons between the surface of the cathodic electrode and the enzyme active sites. Our results demonstrate that the dry printed layers maintained their enzymatic activity even after several months. Furthermore, fuel cell prototypes could be constructed utilising an optimized printed laccase–ABTS layer as the cathode, and printed Zn layer as the anode. Under humidity controlled conditions, a cell voltage between 0.8 and 0.6 V could be maintained for several days under a 2.2 kΩ load. In addition, a corresponding stand-alone cell could be constructed where the cell voltage was maintained for 15 h under a load. These results offer a good starting point for further development of mass-producible, completely enzymatic printed biofuel cells.

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