Development of biocompatible surfaces for sensor applications

Liisa Kivimäki, Hanna Männikkö, Arto Maaninen, Markku Känsäkoski, Kristiina Takkinen, Timo Pulli, Tuija Teerinen, Leena Hakalahti

Research output: Contribution to conferenceConference AbstractScientific

Abstract

New multi scientific and -technological cooperation culture around Oulu region has been created with the help of BioOulu project. In this work new methods to manufacture printed biosensors for point-of-care use have been investigated and developed. The project has been mainly concentrated to the development of biocompatible materials for biosensors. Porous silane based sol-gel materials have been developed in this research project. Biomolecules inside the sol-gel are surrounded by a protecting and stabilizing micro environment which prevents the denaturation of the biomolecules. Several biocompatible matrices were developed via the sol-gel technique. Antibodies were mixed with the sol-gel or coated on top of the sol-gel layer. Biomolecules were detected with fluorescein (FITC) labelled antibodies. By optimizing the sol-gel material process conditions such as precursors and their molar ratio but also reaction time and temperature, the material composition was affected and thereby the behavior of the added biomolecules with the generated materials altered. By the choice of the carrier sol-gel matrix the antibody immobilization effectiveness could be influenced. Antibodies used in this work retained their activity after doping and processing. Doped sol-gel materials were gravure printed and the biomolecules were detected and found to preserve their biological functionality. In the future the goal is to create a biosensor carrier which is industrially exploitable and is suitable for the use in disposable point-of-care analysing devices manufactured by printing techniques. This work was funded by the European Regional Development Fund, the Provincial State Office of Oulu and the city of Oulu.
Original languageEnglish
Publication statusPublished - 2006
MoE publication typeNot Eligible
EventBio Meets Nano and IT 2006 - Oulu, Finland
Duration: 7 Sep 20068 Sep 2006

Conference

ConferenceBio Meets Nano and IT 2006
CountryFinland
CityOulu
Period7/09/068/09/06

Fingerprint

Sol-gels
Biomolecules
Sensors
Antibodies
Biosensors
Denaturation
Silanes
Biomaterials
Printing
Doping (additives)
Processing
Chemical analysis

Cite this

Kivimäki, L., Männikkö, H., Maaninen, A., Känsäkoski, M., Takkinen, K., Pulli, T., ... Hakalahti, L. (2006). Development of biocompatible surfaces for sensor applications. Abstract from Bio Meets Nano and IT 2006, Oulu, Finland.
Kivimäki, Liisa ; Männikkö, Hanna ; Maaninen, Arto ; Känsäkoski, Markku ; Takkinen, Kristiina ; Pulli, Timo ; Teerinen, Tuija ; Hakalahti, Leena. / Development of biocompatible surfaces for sensor applications. Abstract from Bio Meets Nano and IT 2006, Oulu, Finland.
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abstract = "New multi scientific and -technological cooperation culture around Oulu region has been created with the help of BioOulu project. In this work new methods to manufacture printed biosensors for point-of-care use have been investigated and developed. The project has been mainly concentrated to the development of biocompatible materials for biosensors. Porous silane based sol-gel materials have been developed in this research project. Biomolecules inside the sol-gel are surrounded by a protecting and stabilizing micro environment which prevents the denaturation of the biomolecules. Several biocompatible matrices were developed via the sol-gel technique. Antibodies were mixed with the sol-gel or coated on top of the sol-gel layer. Biomolecules were detected with fluorescein (FITC) labelled antibodies. By optimizing the sol-gel material process conditions such as precursors and their molar ratio but also reaction time and temperature, the material composition was affected and thereby the behavior of the added biomolecules with the generated materials altered. By the choice of the carrier sol-gel matrix the antibody immobilization effectiveness could be influenced. Antibodies used in this work retained their activity after doping and processing. Doped sol-gel materials were gravure printed and the biomolecules were detected and found to preserve their biological functionality. In the future the goal is to create a biosensor carrier which is industrially exploitable and is suitable for the use in disposable point-of-care analysing devices manufactured by printing techniques. This work was funded by the European Regional Development Fund, the Provincial State Office of Oulu and the city of Oulu.",
author = "Liisa Kivim{\"a}ki and Hanna M{\"a}nnikk{\"o} and Arto Maaninen and Markku K{\"a}ns{\"a}koski and Kristiina Takkinen and Timo Pulli and Tuija Teerinen and Leena Hakalahti",
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Kivimäki, L, Männikkö, H, Maaninen, A, Känsäkoski, M, Takkinen, K, Pulli, T, Teerinen, T & Hakalahti, L 2006, 'Development of biocompatible surfaces for sensor applications' Bio Meets Nano and IT 2006, Oulu, Finland, 7/09/06 - 8/09/06, .

Development of biocompatible surfaces for sensor applications. / Kivimäki, Liisa; Männikkö, Hanna; Maaninen, Arto; Känsäkoski, Markku; Takkinen, Kristiina; Pulli, Timo; Teerinen, Tuija; Hakalahti, Leena.

2006. Abstract from Bio Meets Nano and IT 2006, Oulu, Finland.

Research output: Contribution to conferenceConference AbstractScientific

TY - CONF

T1 - Development of biocompatible surfaces for sensor applications

AU - Kivimäki, Liisa

AU - Männikkö, Hanna

AU - Maaninen, Arto

AU - Känsäkoski, Markku

AU - Takkinen, Kristiina

AU - Pulli, Timo

AU - Teerinen, Tuija

AU - Hakalahti, Leena

PY - 2006

Y1 - 2006

N2 - New multi scientific and -technological cooperation culture around Oulu region has been created with the help of BioOulu project. In this work new methods to manufacture printed biosensors for point-of-care use have been investigated and developed. The project has been mainly concentrated to the development of biocompatible materials for biosensors. Porous silane based sol-gel materials have been developed in this research project. Biomolecules inside the sol-gel are surrounded by a protecting and stabilizing micro environment which prevents the denaturation of the biomolecules. Several biocompatible matrices were developed via the sol-gel technique. Antibodies were mixed with the sol-gel or coated on top of the sol-gel layer. Biomolecules were detected with fluorescein (FITC) labelled antibodies. By optimizing the sol-gel material process conditions such as precursors and their molar ratio but also reaction time and temperature, the material composition was affected and thereby the behavior of the added biomolecules with the generated materials altered. By the choice of the carrier sol-gel matrix the antibody immobilization effectiveness could be influenced. Antibodies used in this work retained their activity after doping and processing. Doped sol-gel materials were gravure printed and the biomolecules were detected and found to preserve their biological functionality. In the future the goal is to create a biosensor carrier which is industrially exploitable and is suitable for the use in disposable point-of-care analysing devices manufactured by printing techniques. This work was funded by the European Regional Development Fund, the Provincial State Office of Oulu and the city of Oulu.

AB - New multi scientific and -technological cooperation culture around Oulu region has been created with the help of BioOulu project. In this work new methods to manufacture printed biosensors for point-of-care use have been investigated and developed. The project has been mainly concentrated to the development of biocompatible materials for biosensors. Porous silane based sol-gel materials have been developed in this research project. Biomolecules inside the sol-gel are surrounded by a protecting and stabilizing micro environment which prevents the denaturation of the biomolecules. Several biocompatible matrices were developed via the sol-gel technique. Antibodies were mixed with the sol-gel or coated on top of the sol-gel layer. Biomolecules were detected with fluorescein (FITC) labelled antibodies. By optimizing the sol-gel material process conditions such as precursors and their molar ratio but also reaction time and temperature, the material composition was affected and thereby the behavior of the added biomolecules with the generated materials altered. By the choice of the carrier sol-gel matrix the antibody immobilization effectiveness could be influenced. Antibodies used in this work retained their activity after doping and processing. Doped sol-gel materials were gravure printed and the biomolecules were detected and found to preserve their biological functionality. In the future the goal is to create a biosensor carrier which is industrially exploitable and is suitable for the use in disposable point-of-care analysing devices manufactured by printing techniques. This work was funded by the European Regional Development Fund, the Provincial State Office of Oulu and the city of Oulu.

M3 - Conference Abstract

ER -

Kivimäki L, Männikkö H, Maaninen A, Känsäkoski M, Takkinen K, Pulli T et al. Development of biocompatible surfaces for sensor applications. 2006. Abstract from Bio Meets Nano and IT 2006, Oulu, Finland.