Development of continuous two-dimensional asymmetrical flow field-flow fractionation for particles: Principle, instrument development and applications

Matti Jussila, Karina Moslova, Pertti Vastamäki, Marja-Liisa Riekkola

    Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

    Abstract

    Continuous two-dimensional field-flow fractionation (2D-FFF) is a field flow fractionation principle for continuous fractionation of macromolecules and particles [1]. In this technique the separation occur in a thin disc-shaped channel, where a carrier liquid flows radially from the center towards the perimeter of the channel, and a steady stream of the sample solution is introduced continuously at a second inlet close to the center. Under influence of the field, the sample components are separated in radial direction according to the analytical FFF principle. Simultaneously, the lower channel wall is rotating with respect to the stationary upper wall, while a shear-driven flow profile deflects the separated sample components into continuous trajectories that strike off at different angles over the 2D surface. Finally, the sample components are collected at the outer rim of the channel. In this work a new 2D-FFF instrument design with asymmetrical cross flow as a force field was constructed according to the principles of AF4 and the continuous 2D-FFF. Instrument performance was tested using coloured samples. The effect of different flow parameters on fractionation was investigated.
    Original languageEnglish
    Title of host publication18th International Symposium on Field- and Flow-Based Separations (FFF 2016)
    Subtitle of host publicationBook of Abstracts
    Publication statusPublished - 2016
    Event18th International Symposium on Field- and Flow-Based Separations, FFF 2016 - Dresden, Germany
    Duration: 22 May 201626 May 2016
    Conference number: 18

    Conference

    Conference18th International Symposium on Field- and Flow-Based Separations, FFF 2016
    Abbreviated titleFFF 2016
    CountryGermany
    CityDresden
    Period22/05/1626/05/16

    Keywords

    • field-flow fractionation
    • continuous separation
    • particles
    • polymers
    • macromolecules

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    Jussila, M., Moslova, K., Vastamäki, P., & Riekkola, M-L. (2016). Development of continuous two-dimensional asymmetrical flow field-flow fractionation for particles: Principle, instrument development and applications. In 18th International Symposium on Field- and Flow-Based Separations (FFF 2016): Book of Abstracts [P44] http://www.ipfdd.de/fileadmin/user_upload/oea/Book_of_abstracts_2016-05-09.pdf