Abstract
Continuous two-dimensional field-flow fractionation
(2D-FFF) is a field flow fractionation principle for
continuous fractionation of macromolecules and particles
[1]. In this technique the separation occur in a thin
disc-shaped channel, where a carrier liquid flows
radially from the center towards the perimeter of the
channel, and a steady stream of the sample solution is
introduced continuously at a second inlet close to the
center. Under influence of the field, the sample
components are separated in radial direction according to
the analytical FFF principle. Simultaneously, the lower
channel wall is rotating with respect to the stationary
upper wall, while a shear-driven flow profile deflects
the separated sample components into continuous
trajectories that strike off at different angles over the
2D surface. Finally, the sample components are collected
at the outer rim of the channel.
In this work a new 2D-FFF instrument design with
asymmetrical cross flow as a force field was constructed
according to the principles of AF4 and the continuous
2D-FFF. Instrument performance was tested using coloured
samples. The effect of different flow parameters on
fractionation was investigated.
Original language | English |
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Title of host publication | 18th International Symposium on Field- and Flow-Based Separations (FFF 2016) |
Subtitle of host publication | Book of Abstracts |
Publication status | Published - 2016 |
Event | 18th International Symposium on Field- and Flow-Based Separations, FFF 2016 - Dresden, Germany Duration: 22 May 2016 → 26 May 2016 Conference number: 18 |
Conference
Conference | 18th International Symposium on Field- and Flow-Based Separations, FFF 2016 |
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Abbreviated title | FFF 2016 |
Country/Territory | Germany |
City | Dresden |
Period | 22/05/16 → 26/05/16 |
Keywords
- field-flow fractionation
- continuous separation
- particles
- polymers
- macromolecules