Development of in vitro techniques for the important medicinal plant Veratrum californicum

Research output: Contribution to journalArticleScientificpeer-review

12 Citations (Scopus)

Abstract

Veratrum californicum (Liliaceae) is an important monocotyledonous medicinal plant which is the only source of the anticancer compound cyclopamine. An in vitro culture system for somatic embryogenesis and green plant regeneration of Veratrum californicum was developed. Embryogenic calli were induced from mature embryos on induction medium. Five basal media supplemented with different growth regulators were evaluated for embryogenic callus induction, modified MS medium with 4 mg/L picloram showing the best result for embryogenic callus production. Fine suspension cell lines were established by employing friable embryogenic calli as starting material and AA medium and L2 medium as culture media. The suspension cell lines cultured in AA medium with 4 mg/L NAA appeared to be fresh yellow and fast growing. The suspension cells were cryopreserved successfully and recovered at a high rate. Green plants were regenerated from embryogenic calli maintained on solid medium with 73 % regeneration ability (green plants/100 calli) in 27-month-old culture. The in vitro plantlets contained the steroid alkaloids cyclopamine and veratramine. This in vitro system will form the basis for metabolic engineering of Veratrum cells in the context of biotechnological production of pharmaceutically important secondary metabolites.
Original languageEnglish
Pages (from-to)1142-1148
Number of pages7
JournalPlanta Medica
Volume72
Issue number12
DOIs
Publication statusPublished - 2006
MoE publication typeA1 Journal article-refereed

Fingerprint

Veratrum
Bony Callus
Medicinal Plants
Suspensions
Viridiplantae
Picloram
Cells
Metabolic engineering
Metabolites
Alkaloids
Culture Media
Regeneration
Steroids
Liliaceae
Metabolic Engineering
Cell Line
Embryonic Development
In Vitro Techniques
Embryonic Structures
cyclopamine

Keywords

  • Alkaloids
  • cyclopamine
  • veratramine
  • cryopreservation
  • suspension culture
  • plant regeneration

Cite this

@article{898f484552c44bc086f23ab618316411,
title = "Development of in vitro techniques for the important medicinal plant Veratrum californicum",
abstract = "Veratrum californicum (Liliaceae) is an important monocotyledonous medicinal plant which is the only source of the anticancer compound cyclopamine. An in vitro culture system for somatic embryogenesis and green plant regeneration of Veratrum californicum was developed. Embryogenic calli were induced from mature embryos on induction medium. Five basal media supplemented with different growth regulators were evaluated for embryogenic callus induction, modified MS medium with 4 mg/L picloram showing the best result for embryogenic callus production. Fine suspension cell lines were established by employing friable embryogenic calli as starting material and AA medium and L2 medium as culture media. The suspension cell lines cultured in AA medium with 4 mg/L NAA appeared to be fresh yellow and fast growing. The suspension cells were cryopreserved successfully and recovered at a high rate. Green plants were regenerated from embryogenic calli maintained on solid medium with 73 {\%} regeneration ability (green plants/100 calli) in 27-month-old culture. The in vitro plantlets contained the steroid alkaloids cyclopamine and veratramine. This in vitro system will form the basis for metabolic engineering of Veratrum cells in the context of biotechnological production of pharmaceutically important secondary metabolites.",
keywords = "Alkaloids, cyclopamine, veratramine, cryopreservation, suspension culture, plant regeneration",
author = "Rui Ma and Anneli Ritala and Kirsi-Marja Oksman-Caldentey and Heiko Rischer",
year = "2006",
doi = "10.1055/s-2006-946697",
language = "English",
volume = "72",
pages = "1142--1148",
journal = "Planta Medica",
issn = "0032-0943",
publisher = "Georg Thieme Verlag",
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}

Development of in vitro techniques for the important medicinal plant Veratrum californicum. / Ma, Rui; Ritala, Anneli; Oksman-Caldentey, Kirsi-Marja; Rischer, Heiko.

In: Planta Medica, Vol. 72, No. 12, 2006, p. 1142-1148.

Research output: Contribution to journalArticleScientificpeer-review

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AB - Veratrum californicum (Liliaceae) is an important monocotyledonous medicinal plant which is the only source of the anticancer compound cyclopamine. An in vitro culture system for somatic embryogenesis and green plant regeneration of Veratrum californicum was developed. Embryogenic calli were induced from mature embryos on induction medium. Five basal media supplemented with different growth regulators were evaluated for embryogenic callus induction, modified MS medium with 4 mg/L picloram showing the best result for embryogenic callus production. Fine suspension cell lines were established by employing friable embryogenic calli as starting material and AA medium and L2 medium as culture media. The suspension cell lines cultured in AA medium with 4 mg/L NAA appeared to be fresh yellow and fast growing. The suspension cells were cryopreserved successfully and recovered at a high rate. Green plants were regenerated from embryogenic calli maintained on solid medium with 73 % regeneration ability (green plants/100 calli) in 27-month-old culture. The in vitro plantlets contained the steroid alkaloids cyclopamine and veratramine. This in vitro system will form the basis for metabolic engineering of Veratrum cells in the context of biotechnological production of pharmaceutically important secondary metabolites.

KW - Alkaloids

KW - cyclopamine

KW - veratramine

KW - cryopreservation

KW - suspension culture

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