Development of printable bioactive paper containing laccase

Heini Virtanen (Corresponding Author), H. Orelma, Tomi Erho, Maria Smolander

    Research output: Contribution to journalArticleScientificpeer-review

    12 Citations (Scopus)


    Compatibility of Trametes versicolor and Trametes hirsuta laccases was studied with polymers used for flexographic inks. The aim was to produce bioactive paper with ability to change color. Optimum pH for the stability of Trametes versicolor and Trametes hirsuta laccases was determined during storage at room temperature for 60 days. The optimum pH for the stability of both laccases was 8–9. The stabilization effect of flexo printing inks on the enzymes was tested in liquid form and when coated on paper. Sulfo polyester resin HZ1100D stabilized the two laccases both in solution and on paper. For example, Trametes versicolor laccase remained stable for at least 8 weeks when coated with HZ1100D polymer. Furthermore, the adsorption of the flexo inks to cellulose was studied with quartz crystal microbalance with dissipation monitoring (QCM-D). It was observed that HZ1100D also adsorbs well on cellulose over a wide pH range. The results suggested that laccases are well suited to bioactive paper applications. Large scale manufacturing of bioactive paper products by flexo printing would be possible because of the compatibility of laccases with flexo inks.
    Original languageEnglish
    Pages (from-to)1496-1502
    Number of pages6
    JournalProcess Biochemistry
    Issue number10
    Publication statusPublished - 2012
    MoE publication typeA1 Journal article-refereed


    • Laccase stability
    • Flexographic printing
    • Bioactive paper
    • Quartz crystal microbalance


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