Development of specific adsorbents for human tumor necrosis factor-α

Influence of antibody immunobilization on performance and biocompatibility

V. Weber (Corresponding Author), I. Linsberger, M. Ettenauer, F. Loth, Matti Höyhtyä, D. Falkenhagen

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

To develop adsorbents for the specific removal of tumor necrosis factor-α (TNF) in extracorporeal blood purification, cellulose microparticles were functionalized either with a monoclonal anti-TNF antibody (mAb) or with recombinant human antibody fragments (Fab). The TNF binding capacity of the adsorbents was determined with in vitro batch experiments using spiked human plasma (spike:  1200 pg TNF/mL; 1 mg particles in 250 μL plasma). Random immobilization of the full-sized monoclonal antibody to periodate-activated cellulose yielded particles with excellent adsorption capacity (258.1 ± 48.6 pg TNF per mg adsorbent wet weight). No leaching of antibody was detectable, and the adsorbents retained their activity for at least 12 months at 4 °C. We found that the conditions used during immobilization of the antibody (pH, nature of the reducing agent) profoundly influenced the biocompatibility of the resulting adsorbents, especially with respect to activation of the complement system. Particles obtained by random immobilization of the monovalent Fab fragments on periodate-activated cellulose using the same conditions as for immobilization of the mAb exhibited only low adsorption capacity (44 ± 7 pg/mg adsorbent wet weight). Oriented coupling of the Fab fragments on chelate-epoxy cellulose via a C-terminal histidine tag, however, increased the adsorption capacity to 178.3 ± 8.6 pg TNF/mg adsorbent wet weight. Thus, in the case of small, monovalent ligands, the orientation on the carrier is critical to retain full binding activity.
Original languageEnglish
Pages (from-to)1864 - 1870
Number of pages7
JournalBiomacromolecules
Volume6
Issue number4
DOIs
Publication statusPublished - 2005
MoE publication typeA1 Journal article-refereed

Fingerprint

Biocompatibility
Antibodies
Adsorbents
Tumor Necrosis Factor-alpha
Cellulose
Immunoglobulin Fab Fragments
Adsorption
Plasma (human)
Immunoglobulin Fragments
Monoclonal antibodies
Reducing Agents
Reducing agents
human TNF protein
Histidine
Leaching
Purification
Blood
Chemical activation
Ligands
Monoclonal Antibodies

Keywords

  • immobilization
  • antibodies
  • blood
  • adsorbents
  • cancer

Cite this

Weber, V. ; Linsberger, I. ; Ettenauer, M. ; Loth, F. ; Höyhtyä, Matti ; Falkenhagen, D. / Development of specific adsorbents for human tumor necrosis factor-α : Influence of antibody immunobilization on performance and biocompatibility. In: Biomacromolecules. 2005 ; Vol. 6, No. 4. pp. 1864 - 1870.
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abstract = "To develop adsorbents for the specific removal of tumor necrosis factor-α (TNF) in extracorporeal blood purification, cellulose microparticles were functionalized either with a monoclonal anti-TNF antibody (mAb) or with recombinant human antibody fragments (Fab). The TNF binding capacity of the adsorbents was determined with in vitro batch experiments using spiked human plasma (spike:  1200 pg TNF/mL; 1 mg particles in 250 μL plasma). Random immobilization of the full-sized monoclonal antibody to periodate-activated cellulose yielded particles with excellent adsorption capacity (258.1 ± 48.6 pg TNF per mg adsorbent wet weight). No leaching of antibody was detectable, and the adsorbents retained their activity for at least 12 months at 4 °C. We found that the conditions used during immobilization of the antibody (pH, nature of the reducing agent) profoundly influenced the biocompatibility of the resulting adsorbents, especially with respect to activation of the complement system. Particles obtained by random immobilization of the monovalent Fab fragments on periodate-activated cellulose using the same conditions as for immobilization of the mAb exhibited only low adsorption capacity (44 ± 7 pg/mg adsorbent wet weight). Oriented coupling of the Fab fragments on chelate-epoxy cellulose via a C-terminal histidine tag, however, increased the adsorption capacity to 178.3 ± 8.6 pg TNF/mg adsorbent wet weight. Thus, in the case of small, monovalent ligands, the orientation on the carrier is critical to retain full binding activity.",
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Development of specific adsorbents for human tumor necrosis factor-α : Influence of antibody immunobilization on performance and biocompatibility. / Weber, V. (Corresponding Author); Linsberger, I.; Ettenauer, M.; Loth, F.; Höyhtyä, Matti; Falkenhagen, D.

In: Biomacromolecules, Vol. 6, No. 4, 2005, p. 1864 - 1870.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Development of specific adsorbents for human tumor necrosis factor-α

T2 - Influence of antibody immunobilization on performance and biocompatibility

AU - Weber, V.

AU - Linsberger, I.

AU - Ettenauer, M.

AU - Loth, F.

AU - Höyhtyä, Matti

AU - Falkenhagen, D.

PY - 2005

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N2 - To develop adsorbents for the specific removal of tumor necrosis factor-α (TNF) in extracorporeal blood purification, cellulose microparticles were functionalized either with a monoclonal anti-TNF antibody (mAb) or with recombinant human antibody fragments (Fab). The TNF binding capacity of the adsorbents was determined with in vitro batch experiments using spiked human plasma (spike:  1200 pg TNF/mL; 1 mg particles in 250 μL plasma). Random immobilization of the full-sized monoclonal antibody to periodate-activated cellulose yielded particles with excellent adsorption capacity (258.1 ± 48.6 pg TNF per mg adsorbent wet weight). No leaching of antibody was detectable, and the adsorbents retained their activity for at least 12 months at 4 °C. We found that the conditions used during immobilization of the antibody (pH, nature of the reducing agent) profoundly influenced the biocompatibility of the resulting adsorbents, especially with respect to activation of the complement system. Particles obtained by random immobilization of the monovalent Fab fragments on periodate-activated cellulose using the same conditions as for immobilization of the mAb exhibited only low adsorption capacity (44 ± 7 pg/mg adsorbent wet weight). Oriented coupling of the Fab fragments on chelate-epoxy cellulose via a C-terminal histidine tag, however, increased the adsorption capacity to 178.3 ± 8.6 pg TNF/mg adsorbent wet weight. Thus, in the case of small, monovalent ligands, the orientation on the carrier is critical to retain full binding activity.

AB - To develop adsorbents for the specific removal of tumor necrosis factor-α (TNF) in extracorporeal blood purification, cellulose microparticles were functionalized either with a monoclonal anti-TNF antibody (mAb) or with recombinant human antibody fragments (Fab). The TNF binding capacity of the adsorbents was determined with in vitro batch experiments using spiked human plasma (spike:  1200 pg TNF/mL; 1 mg particles in 250 μL plasma). Random immobilization of the full-sized monoclonal antibody to periodate-activated cellulose yielded particles with excellent adsorption capacity (258.1 ± 48.6 pg TNF per mg adsorbent wet weight). No leaching of antibody was detectable, and the adsorbents retained their activity for at least 12 months at 4 °C. We found that the conditions used during immobilization of the antibody (pH, nature of the reducing agent) profoundly influenced the biocompatibility of the resulting adsorbents, especially with respect to activation of the complement system. Particles obtained by random immobilization of the monovalent Fab fragments on periodate-activated cellulose using the same conditions as for immobilization of the mAb exhibited only low adsorption capacity (44 ± 7 pg/mg adsorbent wet weight). Oriented coupling of the Fab fragments on chelate-epoxy cellulose via a C-terminal histidine tag, however, increased the adsorption capacity to 178.3 ± 8.6 pg TNF/mg adsorbent wet weight. Thus, in the case of small, monovalent ligands, the orientation on the carrier is critical to retain full binding activity.

KW - immobilization

KW - antibodies

KW - blood

KW - adsorbents

KW - cancer

U2 - 10.1021/bm040074t

DO - 10.1021/bm040074t

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VL - 6

SP - 1864

EP - 1870

JO - Biomacromolecules

JF - Biomacromolecules

SN - 1525-7797

IS - 4

ER -