Development of suberin fatty acids and chloramphenicol-loaded antimicrobial electrospun nanofibrous mats intended for wound therapy

Ingrid Tamm, Jyrki Heinämäki, Ivo Laidmäe, Liisi Rammo, Urve Paaver, Sveinung G. Ingebrigtsen, Natasa Skalko-Basnet, Anna Halenius, Jouko Yliruusi, Pauliina Pitkänen, Sami Alakurtti, Karin Kogermann (Corresponding Author)

    Research output: Contribution to journalArticleScientificpeer-review

    7 Citations (Scopus)

    Abstract

    Suberin fatty acids (SFAs) isolated from outer birch bark were investigated as an antimicrobial agent and biomaterial in nanofibrous mats intended for wound treatment. Electrospinning (ES) was used in preparing the composite nonwoven nanomats containing chloramphenicol (CAM; as a primary antimicrobial drug), SFAs, and polyvinylpyrrolidone (as a carrier polymer for ES). The X-ray powder diffraction, differential scanning calorimetry, scanning electron microscopy, atomic force microscopy, and texture analysis were used for the physicochemical and mechanical characterization of the nanomats. ES produced nanofibrous mats with uniform structure and with an average fiber diameter ranging from 370 to 425 nm. Microcrystalline SFAs and crystalline CAM were found to undergo a solid-state transformation during ES processing. The ES process caused also the loss of CAM in the final nanofibers. In the texture analysis, the SFAs containing nanofibers exhibited significantly higher maximum detachment force to an isolated pig skin (p < 0.05) than that obtained with the reference nanofibers. CAM exists in an amorphous form in the nanofibers which needs to be taken into account in controlling the physical storage stability. In conclusion, homogeneous composite nanofibrous mats for wound healing can be electrospun from the ternary mixture(s) of CAM, SFAs, and polyvinylpyrrolidone.
    Original languageEnglish
    Pages (from-to)1239-1247
    JournalJournal of Pharmaceutical Sciences
    Volume105
    Issue number3
    DOIs
    Publication statusPublished - 2016
    MoE publication typeA1 Journal article-refereed

    Fingerprint

    Nanofibers
    Chloramphenicol
    Fatty Acids
    Wounds and Injuries
    Povidone
    Powder Diffraction
    Therapeutics
    Betula
    Atomic Force Microscopy
    Differential Scanning Calorimetry
    Biocompatible Materials
    Anti-Infective Agents
    X-Ray Diffraction
    Electron Scanning Microscopy
    Wound Healing
    Polymers
    Swine
    suberin
    Skin
    Pharmaceutical Preparations

    Keywords

    • antimicrobial agent/drug
    • electrospun nanofibers
    • nanotechnology
    • nonwoven nanomats
    • physical characterization
    • polymeric biomaterials
    • polymeric drug carrier
    • solvent evaporation
    • suberin fatty acids
    • wound therapy

    Cite this

    Tamm, Ingrid ; Heinämäki, Jyrki ; Laidmäe, Ivo ; Rammo, Liisi ; Paaver, Urve ; Ingebrigtsen, Sveinung G. ; Skalko-Basnet, Natasa ; Halenius, Anna ; Yliruusi, Jouko ; Pitkänen, Pauliina ; Alakurtti, Sami ; Kogermann, Karin. / Development of suberin fatty acids and chloramphenicol-loaded antimicrobial electrospun nanofibrous mats intended for wound therapy. In: Journal of Pharmaceutical Sciences. 2016 ; Vol. 105, No. 3. pp. 1239-1247.
    @article{391828b6ca534e3496f9ff9e9095b210,
    title = "Development of suberin fatty acids and chloramphenicol-loaded antimicrobial electrospun nanofibrous mats intended for wound therapy",
    abstract = "Suberin fatty acids (SFAs) isolated from outer birch bark were investigated as an antimicrobial agent and biomaterial in nanofibrous mats intended for wound treatment. Electrospinning (ES) was used in preparing the composite nonwoven nanomats containing chloramphenicol (CAM; as a primary antimicrobial drug), SFAs, and polyvinylpyrrolidone (as a carrier polymer for ES). The X-ray powder diffraction, differential scanning calorimetry, scanning electron microscopy, atomic force microscopy, and texture analysis were used for the physicochemical and mechanical characterization of the nanomats. ES produced nanofibrous mats with uniform structure and with an average fiber diameter ranging from 370 to 425 nm. Microcrystalline SFAs and crystalline CAM were found to undergo a solid-state transformation during ES processing. The ES process caused also the loss of CAM in the final nanofibers. In the texture analysis, the SFAs containing nanofibers exhibited significantly higher maximum detachment force to an isolated pig skin (p < 0.05) than that obtained with the reference nanofibers. CAM exists in an amorphous form in the nanofibers which needs to be taken into account in controlling the physical storage stability. In conclusion, homogeneous composite nanofibrous mats for wound healing can be electrospun from the ternary mixture(s) of CAM, SFAs, and polyvinylpyrrolidone.",
    keywords = "antimicrobial agent/drug, electrospun nanofibers, nanotechnology, nonwoven nanomats, physical characterization, polymeric biomaterials, polymeric drug carrier, solvent evaporation, suberin fatty acids, wound therapy",
    author = "Ingrid Tamm and Jyrki Hein{\"a}m{\"a}ki and Ivo Laidm{\"a}e and Liisi Rammo and Urve Paaver and Ingebrigtsen, {Sveinung G.} and Natasa Skalko-Basnet and Anna Halenius and Jouko Yliruusi and Pauliina Pitk{\"a}nen and Sami Alakurtti and Karin Kogermann",
    year = "2016",
    doi = "10.1016/j.xphs.2015.12.025",
    language = "English",
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    pages = "1239--1247",
    journal = "Journal of Pharmaceutical Sciences",
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    Tamm, I, Heinämäki, J, Laidmäe, I, Rammo, L, Paaver, U, Ingebrigtsen, SG, Skalko-Basnet, N, Halenius, A, Yliruusi, J, Pitkänen, P, Alakurtti, S & Kogermann, K 2016, 'Development of suberin fatty acids and chloramphenicol-loaded antimicrobial electrospun nanofibrous mats intended for wound therapy', Journal of Pharmaceutical Sciences, vol. 105, no. 3, pp. 1239-1247. https://doi.org/10.1016/j.xphs.2015.12.025

    Development of suberin fatty acids and chloramphenicol-loaded antimicrobial electrospun nanofibrous mats intended for wound therapy. / Tamm, Ingrid; Heinämäki, Jyrki; Laidmäe, Ivo; Rammo, Liisi; Paaver, Urve; Ingebrigtsen, Sveinung G.; Skalko-Basnet, Natasa; Halenius, Anna; Yliruusi, Jouko; Pitkänen, Pauliina; Alakurtti, Sami; Kogermann, Karin (Corresponding Author).

    In: Journal of Pharmaceutical Sciences, Vol. 105, No. 3, 2016, p. 1239-1247.

    Research output: Contribution to journalArticleScientificpeer-review

    TY - JOUR

    T1 - Development of suberin fatty acids and chloramphenicol-loaded antimicrobial electrospun nanofibrous mats intended for wound therapy

    AU - Tamm, Ingrid

    AU - Heinämäki, Jyrki

    AU - Laidmäe, Ivo

    AU - Rammo, Liisi

    AU - Paaver, Urve

    AU - Ingebrigtsen, Sveinung G.

    AU - Skalko-Basnet, Natasa

    AU - Halenius, Anna

    AU - Yliruusi, Jouko

    AU - Pitkänen, Pauliina

    AU - Alakurtti, Sami

    AU - Kogermann, Karin

    PY - 2016

    Y1 - 2016

    N2 - Suberin fatty acids (SFAs) isolated from outer birch bark were investigated as an antimicrobial agent and biomaterial in nanofibrous mats intended for wound treatment. Electrospinning (ES) was used in preparing the composite nonwoven nanomats containing chloramphenicol (CAM; as a primary antimicrobial drug), SFAs, and polyvinylpyrrolidone (as a carrier polymer for ES). The X-ray powder diffraction, differential scanning calorimetry, scanning electron microscopy, atomic force microscopy, and texture analysis were used for the physicochemical and mechanical characterization of the nanomats. ES produced nanofibrous mats with uniform structure and with an average fiber diameter ranging from 370 to 425 nm. Microcrystalline SFAs and crystalline CAM were found to undergo a solid-state transformation during ES processing. The ES process caused also the loss of CAM in the final nanofibers. In the texture analysis, the SFAs containing nanofibers exhibited significantly higher maximum detachment force to an isolated pig skin (p < 0.05) than that obtained with the reference nanofibers. CAM exists in an amorphous form in the nanofibers which needs to be taken into account in controlling the physical storage stability. In conclusion, homogeneous composite nanofibrous mats for wound healing can be electrospun from the ternary mixture(s) of CAM, SFAs, and polyvinylpyrrolidone.

    AB - Suberin fatty acids (SFAs) isolated from outer birch bark were investigated as an antimicrobial agent and biomaterial in nanofibrous mats intended for wound treatment. Electrospinning (ES) was used in preparing the composite nonwoven nanomats containing chloramphenicol (CAM; as a primary antimicrobial drug), SFAs, and polyvinylpyrrolidone (as a carrier polymer for ES). The X-ray powder diffraction, differential scanning calorimetry, scanning electron microscopy, atomic force microscopy, and texture analysis were used for the physicochemical and mechanical characterization of the nanomats. ES produced nanofibrous mats with uniform structure and with an average fiber diameter ranging from 370 to 425 nm. Microcrystalline SFAs and crystalline CAM were found to undergo a solid-state transformation during ES processing. The ES process caused also the loss of CAM in the final nanofibers. In the texture analysis, the SFAs containing nanofibers exhibited significantly higher maximum detachment force to an isolated pig skin (p < 0.05) than that obtained with the reference nanofibers. CAM exists in an amorphous form in the nanofibers which needs to be taken into account in controlling the physical storage stability. In conclusion, homogeneous composite nanofibrous mats for wound healing can be electrospun from the ternary mixture(s) of CAM, SFAs, and polyvinylpyrrolidone.

    KW - antimicrobial agent/drug

    KW - electrospun nanofibers

    KW - nanotechnology

    KW - nonwoven nanomats

    KW - physical characterization

    KW - polymeric biomaterials

    KW - polymeric drug carrier

    KW - solvent evaporation

    KW - suberin fatty acids

    KW - wound therapy

    U2 - 10.1016/j.xphs.2015.12.025

    DO - 10.1016/j.xphs.2015.12.025

    M3 - Article

    VL - 105

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    EP - 1247

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    JF - Journal of Pharmaceutical Sciences

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    IS - 3

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