Abstract
| Original language | English |
|---|---|
| Title of host publication | 3rd European Federation of Biotechnology Conference |
| Subtitle of host publication | Physiology of Yeasts and Filamentous Fungi PYFF3 |
| Place of Publication | Espoo |
| Publisher | VTT Technical Research Centre of Finland |
| Pages | 149 |
| ISBN (Electronic) | 978-951-38-6314-2 |
| ISBN (Print) | 978-951-38-6313-5 |
| Publication status | Published - 2007 |
| Event | 3rd European Federation of Biotechnology Conference : Physiology of Yeasts and Filamentous Fungi - Helsinki, Finland Duration: 13 Jun 2007 → 16 Jun 2007 |
Publication series
| Series | VTT Symposium |
|---|---|
| Number | 245 |
| ISSN | 0357-9387 |
Conference
| Conference | 3rd European Federation of Biotechnology Conference |
|---|---|
| Abbreviated title | PYFF3 |
| Country | Finland |
| City | Helsinki |
| Period | 13/06/07 → 16/06/07 |
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Development of Trichoderma reesei transformation technology with the aid of an episomal vector system. / Aro, Nina; Westerholm-Parvinen, Ann; Penttilä, Merja; Saloheimo, Markku.
3rd European Federation of Biotechnology Conference: Physiology of Yeasts and Filamentous Fungi PYFF3. Espoo : VTT Technical Research Centre of Finland, 2007. p. 149 P93 (VTT Symposium; No. 245).Research output: Chapter in Book/Report/Conference proceeding › Conference abstract in proceedings › Scientific
TY - CHAP
T1 - Development of Trichoderma reesei transformation technology with the aid of an episomal vector system
AU - Aro, Nina
AU - Westerholm-Parvinen, Ann
AU - Penttilä, Merja
AU - Saloheimo, Markku
PY - 2007
Y1 - 2007
N2 - In conventional transformation procedures of filamentous fungi the transformation frequencies have been modest and only integrative vectors have been available. In modern fungal molecular biology, however, screening approaches with the help episomal vectors have advanced the progress of the field greatly. For example, in enzyme discovery or protein engineering it is beneficial to be able to screen for new enzymes or improved mutant enzymes in the ultimate production host of the enzyme. Episomal vector systems have been developed for a few fungal species including the Aspergilli, but Trichoderma reesei has suffered from a lack of such a system. We have developed T. reesei transformation technology for genetic and enzyme screening purposes. The transformation procedure was optimised to improve the transformation frequencies of any vectors. Telomeric episomal vectors were used, and with these we obtained transformation frequencies that are far above those obtained with integrative vectors and are feasible for genetic and enzyme screening purposes. Procedures for plasmid rescue, colony picking and microtiter plate cultivation were also developed. The use of the novel transformation system in enzyme and genetic screening experiments will be discussed.
AB - In conventional transformation procedures of filamentous fungi the transformation frequencies have been modest and only integrative vectors have been available. In modern fungal molecular biology, however, screening approaches with the help episomal vectors have advanced the progress of the field greatly. For example, in enzyme discovery or protein engineering it is beneficial to be able to screen for new enzymes or improved mutant enzymes in the ultimate production host of the enzyme. Episomal vector systems have been developed for a few fungal species including the Aspergilli, but Trichoderma reesei has suffered from a lack of such a system. We have developed T. reesei transformation technology for genetic and enzyme screening purposes. The transformation procedure was optimised to improve the transformation frequencies of any vectors. Telomeric episomal vectors were used, and with these we obtained transformation frequencies that are far above those obtained with integrative vectors and are feasible for genetic and enzyme screening purposes. Procedures for plasmid rescue, colony picking and microtiter plate cultivation were also developed. The use of the novel transformation system in enzyme and genetic screening experiments will be discussed.
M3 - Conference abstract in proceedings
SN - 978-951-38-6313-5
T3 - VTT Symposium
SP - 149
BT - 3rd European Federation of Biotechnology Conference
PB - VTT Technical Research Centre of Finland
CY - Espoo
ER -