TY - JOUR
T1 - Diatom-inspired skeletonisation of insulin - Mechanistic insights into crystallisation and extracellular bioactivity
AU - Véliz, Diosangeles
AU - Alam, C.
AU - Nietzel, T.
AU - Wyborski, R.
AU - Rivero-Müller, A.
AU - Alam, P.
PY - 2015
Y1 - 2015
N2 - In this paper, we encage insulin within calcium carbonate by means of a biomineralisation process. We find that both dogbone and crossbone morphologies develop during the crystallisation process. The crystals break down into small nanocrystals after prolonged immersion in phosphate buffer solution, which adhere extracellularly to mammalian cells without causing any observable damage or early cell-death. The mechanisms behind calcium carbonate encaging of single insulin monomers are detailed. This communication elucidates a novel, diatom-inspired approach to the mineral skeletonisation of insulin.
AB - In this paper, we encage insulin within calcium carbonate by means of a biomineralisation process. We find that both dogbone and crossbone morphologies develop during the crystallisation process. The crystals break down into small nanocrystals after prolonged immersion in phosphate buffer solution, which adhere extracellularly to mammalian cells without causing any observable damage or early cell-death. The mechanisms behind calcium carbonate encaging of single insulin monomers are detailed. This communication elucidates a novel, diatom-inspired approach to the mineral skeletonisation of insulin.
UR - https://www.scopus.com/pages/publications/84934894656
U2 - 10.1016/j.colsurfb.2015.05.047
DO - 10.1016/j.colsurfb.2015.05.047
M3 - Article
SN - 0927-7765
VL - 133
SP - 140
EP - 147
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
ER -
