Dietary carbohydrate modification alters serum metabolic profiles in individuals with the metabolic syndrome

Maria Lankinen (Corresponding Author), U. Schwab, Peddinti Gopalacharyulu, Tuulikki Seppänen-Laakso, Laxman Yetukuri, Marko Sysi-Aho, P. Kallio, Tapani Suortti, D. E. Laaksonen, H. Gylling, Kaisa Poutanen, M. Kolehmainen, Matej Orešič

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Background and aims

Whole-grain cereals and diets with a low glycemic index may protect against the development of type 2 diabetes and heart disease, but the mechanisms are poorly understood. We studied the effect of carbohydrate modification on serum metabolic profiles, including lipids and branched chain amino acids, and dependencies between these and specific gene expression pathways in adipose tissue.

Methods and results

Twenty subjects with metabolic syndrome were selected from the larger FUNGENUT study population, randomized either to a diet high in oat and wheat bread and potato (OWP) or rye bread and pasta (RP). Serum metabolomics analyses were performed using ultra-performance liquid chromatography coupled to electrospray ionization mass spectrometry (UPLC/MS), gas chromatography (GC) and UPLC. In the OWP group multiple proinflammatory lysophosphatidylcholines increased, while in the RP group docosahexaenoic acid (DHA 22:6n-3) increased and isoleucine decreased. mRNA expression of stress reactions- and adipose tissue differentiation-related genes were up-regulated in adipose tissue in the OWP group. In the RP group, however, pathways related to stress reactions and insulin signaling and energy metabolism were down-regulated. The lipid profiles had the strongest association with the changes in the adipose tissue differentiation pathway when using the elastic net regression model of the lipidomic profiles on selected pathways.

Conclusion

Our results suggest that the dietary carbohydrate modification alters the serum metabolic profile, especially in lysoPC species, and may, thus, contribute to proinflammatory processes which in turn promote adverse changes in insulin and glucose metabolism.

Original languageEnglish
Pages (from-to)249-257
Number of pages9
JournalNutrition, Metabolism and Cardiovascular Diseases
Volume20
Issue number4
DOIs
Publication statusPublished - 2010
MoE publication typeA1 Journal article-refereed

Fingerprint

Dietary Carbohydrates
Diet Therapy
Metabolome
Bread
Adipose Tissue
Solanum tuberosum
Triticum
Serum
Insulin
Diet
Glycemic Index
Lipids
Branched Chain Amino Acids
Lysophosphatidylcholines
Metabolomics
Electrospray Ionization Mass Spectrometry
Docosahexaenoic Acids
Isoleucine
Liquid Chromatography
Gas Chromatography

Keywords

  • Metabolic syndrome
  • diet
  • metabolomics
  • lipidomics

Cite this

Lankinen, Maria ; Schwab, U. ; Gopalacharyulu, Peddinti ; Seppänen-Laakso, Tuulikki ; Yetukuri, Laxman ; Sysi-Aho, Marko ; Kallio, P. ; Suortti, Tapani ; Laaksonen, D. E. ; Gylling, H. ; Poutanen, Kaisa ; Kolehmainen, M. ; Orešič, Matej. / Dietary carbohydrate modification alters serum metabolic profiles in individuals with the metabolic syndrome. In: Nutrition, Metabolism and Cardiovascular Diseases. 2010 ; Vol. 20, No. 4. pp. 249-257.
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title = "Dietary carbohydrate modification alters serum metabolic profiles in individuals with the metabolic syndrome",
abstract = "Background and aimsWhole-grain cereals and diets with a low glycemic index may protect against the development of type 2 diabetes and heart disease, but the mechanisms are poorly understood. We studied the effect of carbohydrate modification on serum metabolic profiles, including lipids and branched chain amino acids, and dependencies between these and specific gene expression pathways in adipose tissue.Methods and resultsTwenty subjects with metabolic syndrome were selected from the larger FUNGENUT study population, randomized either to a diet high in oat and wheat bread and potato (OWP) or rye bread and pasta (RP). Serum metabolomics analyses were performed using ultra-performance liquid chromatography coupled to electrospray ionization mass spectrometry (UPLC/MS), gas chromatography (GC) and UPLC. In the OWP group multiple proinflammatory lysophosphatidylcholines increased, while in the RP group docosahexaenoic acid (DHA 22:6n-3) increased and isoleucine decreased. mRNA expression of stress reactions- and adipose tissue differentiation-related genes were up-regulated in adipose tissue in the OWP group. In the RP group, however, pathways related to stress reactions and insulin signaling and energy metabolism were down-regulated. The lipid profiles had the strongest association with the changes in the adipose tissue differentiation pathway when using the elastic net regression model of the lipidomic profiles on selected pathways.ConclusionOur results suggest that the dietary carbohydrate modification alters the serum metabolic profile, especially in lysoPC species, and may, thus, contribute to proinflammatory processes which in turn promote adverse changes in insulin and glucose metabolism.",
keywords = "Metabolic syndrome, diet, metabolomics, lipidomics",
author = "Maria Lankinen and U. Schwab and Peddinti Gopalacharyulu and Tuulikki Sepp{\"a}nen-Laakso and Laxman Yetukuri and Marko Sysi-Aho and P. Kallio and Tapani Suortti and Laaksonen, {D. E.} and H. Gylling and Kaisa Poutanen and M. Kolehmainen and Matej Orešič",
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language = "English",
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Lankinen, M, Schwab, U, Gopalacharyulu, P, Seppänen-Laakso, T, Yetukuri, L, Sysi-Aho, M, Kallio, P, Suortti, T, Laaksonen, DE, Gylling, H, Poutanen, K, Kolehmainen, M & Orešič, M 2010, 'Dietary carbohydrate modification alters serum metabolic profiles in individuals with the metabolic syndrome', Nutrition, Metabolism and Cardiovascular Diseases, vol. 20, no. 4, pp. 249-257. https://doi.org/10.1016/j.numecd.2009.04.009

Dietary carbohydrate modification alters serum metabolic profiles in individuals with the metabolic syndrome. / Lankinen, Maria (Corresponding Author); Schwab, U.; Gopalacharyulu, Peddinti; Seppänen-Laakso, Tuulikki; Yetukuri, Laxman; Sysi-Aho, Marko; Kallio, P.; Suortti, Tapani; Laaksonen, D. E.; Gylling, H.; Poutanen, Kaisa; Kolehmainen, M.; Orešič, Matej.

In: Nutrition, Metabolism and Cardiovascular Diseases, Vol. 20, No. 4, 2010, p. 249-257.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Dietary carbohydrate modification alters serum metabolic profiles in individuals with the metabolic syndrome

AU - Lankinen, Maria

AU - Schwab, U.

AU - Gopalacharyulu, Peddinti

AU - Seppänen-Laakso, Tuulikki

AU - Yetukuri, Laxman

AU - Sysi-Aho, Marko

AU - Kallio, P.

AU - Suortti, Tapani

AU - Laaksonen, D. E.

AU - Gylling, H.

AU - Poutanen, Kaisa

AU - Kolehmainen, M.

AU - Orešič, Matej

PY - 2010

Y1 - 2010

N2 - Background and aimsWhole-grain cereals and diets with a low glycemic index may protect against the development of type 2 diabetes and heart disease, but the mechanisms are poorly understood. We studied the effect of carbohydrate modification on serum metabolic profiles, including lipids and branched chain amino acids, and dependencies between these and specific gene expression pathways in adipose tissue.Methods and resultsTwenty subjects with metabolic syndrome were selected from the larger FUNGENUT study population, randomized either to a diet high in oat and wheat bread and potato (OWP) or rye bread and pasta (RP). Serum metabolomics analyses were performed using ultra-performance liquid chromatography coupled to electrospray ionization mass spectrometry (UPLC/MS), gas chromatography (GC) and UPLC. In the OWP group multiple proinflammatory lysophosphatidylcholines increased, while in the RP group docosahexaenoic acid (DHA 22:6n-3) increased and isoleucine decreased. mRNA expression of stress reactions- and adipose tissue differentiation-related genes were up-regulated in adipose tissue in the OWP group. In the RP group, however, pathways related to stress reactions and insulin signaling and energy metabolism were down-regulated. The lipid profiles had the strongest association with the changes in the adipose tissue differentiation pathway when using the elastic net regression model of the lipidomic profiles on selected pathways.ConclusionOur results suggest that the dietary carbohydrate modification alters the serum metabolic profile, especially in lysoPC species, and may, thus, contribute to proinflammatory processes which in turn promote adverse changes in insulin and glucose metabolism.

AB - Background and aimsWhole-grain cereals and diets with a low glycemic index may protect against the development of type 2 diabetes and heart disease, but the mechanisms are poorly understood. We studied the effect of carbohydrate modification on serum metabolic profiles, including lipids and branched chain amino acids, and dependencies between these and specific gene expression pathways in adipose tissue.Methods and resultsTwenty subjects with metabolic syndrome were selected from the larger FUNGENUT study population, randomized either to a diet high in oat and wheat bread and potato (OWP) or rye bread and pasta (RP). Serum metabolomics analyses were performed using ultra-performance liquid chromatography coupled to electrospray ionization mass spectrometry (UPLC/MS), gas chromatography (GC) and UPLC. In the OWP group multiple proinflammatory lysophosphatidylcholines increased, while in the RP group docosahexaenoic acid (DHA 22:6n-3) increased and isoleucine decreased. mRNA expression of stress reactions- and adipose tissue differentiation-related genes were up-regulated in adipose tissue in the OWP group. In the RP group, however, pathways related to stress reactions and insulin signaling and energy metabolism were down-regulated. The lipid profiles had the strongest association with the changes in the adipose tissue differentiation pathway when using the elastic net regression model of the lipidomic profiles on selected pathways.ConclusionOur results suggest that the dietary carbohydrate modification alters the serum metabolic profile, especially in lysoPC species, and may, thus, contribute to proinflammatory processes which in turn promote adverse changes in insulin and glucose metabolism.

KW - Metabolic syndrome

KW - diet

KW - metabolomics

KW - lipidomics

U2 - 10.1016/j.numecd.2009.04.009

DO - 10.1016/j.numecd.2009.04.009

M3 - Article

VL - 20

SP - 249

EP - 257

JO - Nutrition, Metabolism and Cardiovascular Diseases

JF - Nutrition, Metabolism and Cardiovascular Diseases

SN - 0939-4753

IS - 4

ER -