Dietary carbohydrate modification induces alterations in gene expression in abdominal subcutaneous adipose tissue in persons with the metabolic syndrome: The FUNGENUT Study

Petteri Kallio, Marjukka Kolehmainen (Corresponding Author), David E. Laaksonen, Jani Kekäläinen, Titta Salopuro, Katariina Sivenius, Leena Pulkkinen, Hannu M. Mykkänen, Leo Niskanen, Matti Uusitupa, Kaisa Poutanen

Research output: Contribution to journalArticleScientific

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Abstract

Background: Diets rich in whole-grain cereals and foods with a low glycemic index may protect against type 2 diabetes, but the underlying molecular mechanisms are unknown. Objective: The main objective was to test whether 2 different carbohydrate modifications—a rye-pasta diet characterized by a low postprandial insulin response and an oat-wheat-potato diet characterized by a high postprandial insulin response—affect gene expression in subcutaneous adipose tissue (SAT) in persons with the metabolic syndrome. Design: We assessed the effect of carbohydrate modification on SAT gene expression in 47 subjects [24 men and 23 women with a mean (±SD) age of 55 ± 6 y] with the features of the metabolic syndrome in a parallel study design. The subjects had a mean (±SD) body mass index (kg/m2) of 32.1 ± 3.8 and a 2-h plasma glucose concentration of 8.0 ± 2.3 mmol/L. Adipose tissue biopsies were performed, and oral-glucose-tolerance tests and other biochemical measurements were conducted before and after the intervention. Results: We detected 71 down-regulated genes in the rye-pasta group, including genes linked to insulin signaling and apoptosis. In contrast, the 12-wk oat-wheat-potato diet up-regulated 62 genes related to stress, cytokine-chemokine–mediated immunity, and the interleukin pathway. The insulinogenic index improved after the rye-pasta diet (P = 0.004) but not after the oat-wheat-potato diet. Body weight was unchanged in both groups. Conclusions: Dietary carbohydrate modification with rye and pasta or oat, wheat, and potato differentially modulates the gene expression profile in abdominal subcutaneous adipose tissue, even in the absence of weight loss.
Original languageEnglish
Pages (from-to)1417-1427
JournalThe American journal of clinical nutrition
Volume85
Issue number5
DOIs
Publication statusPublished - 2007
MoE publication typeB1 Article in a scientific magazine

Fingerprint

Abdominal Subcutaneous Fat
Dietary Carbohydrates
Diet Therapy
Solanum tuberosum
Diet
Gene Expression
Triticum
Subcutaneous Fat
Insulin
Carbohydrates
Genes
Glycemic Index
Interleukins
Glucose Tolerance Test
Transcriptome
Type 2 Diabetes Mellitus
Adipose Tissue
Weight Loss
Immunity
Body Mass Index

Keywords

  • Gene-nutrient interactions
  • metabolic syndrome
  • insulin resistance
  • microarray
  • adipose tissue
  • diet intervention
  • insulinemic response
  • rye
  • oat
  • wheat

Cite this

Kallio, Petteri ; Kolehmainen, Marjukka ; Laaksonen, David E. ; Kekäläinen, Jani ; Salopuro, Titta ; Sivenius, Katariina ; Pulkkinen, Leena ; Mykkänen, Hannu M. ; Niskanen, Leo ; Uusitupa, Matti ; Poutanen, Kaisa. / Dietary carbohydrate modification induces alterations in gene expression in abdominal subcutaneous adipose tissue in persons with the metabolic syndrome: The FUNGENUT Study. In: The American journal of clinical nutrition. 2007 ; Vol. 85, No. 5. pp. 1417-1427.
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abstract = "Background: Diets rich in whole-grain cereals and foods with a low glycemic index may protect against type 2 diabetes, but the underlying molecular mechanisms are unknown. Objective: The main objective was to test whether 2 different carbohydrate modifications—a rye-pasta diet characterized by a low postprandial insulin response and an oat-wheat-potato diet characterized by a high postprandial insulin response—affect gene expression in subcutaneous adipose tissue (SAT) in persons with the metabolic syndrome. Design: We assessed the effect of carbohydrate modification on SAT gene expression in 47 subjects [24 men and 23 women with a mean (±SD) age of 55 ± 6 y] with the features of the metabolic syndrome in a parallel study design. The subjects had a mean (±SD) body mass index (kg/m2) of 32.1 ± 3.8 and a 2-h plasma glucose concentration of 8.0 ± 2.3 mmol/L. Adipose tissue biopsies were performed, and oral-glucose-tolerance tests and other biochemical measurements were conducted before and after the intervention. Results: We detected 71 down-regulated genes in the rye-pasta group, including genes linked to insulin signaling and apoptosis. In contrast, the 12-wk oat-wheat-potato diet up-regulated 62 genes related to stress, cytokine-chemokine–mediated immunity, and the interleukin pathway. The insulinogenic index improved after the rye-pasta diet (P = 0.004) but not after the oat-wheat-potato diet. Body weight was unchanged in both groups. Conclusions: Dietary carbohydrate modification with rye and pasta or oat, wheat, and potato differentially modulates the gene expression profile in abdominal subcutaneous adipose tissue, even in the absence of weight loss.",
keywords = "Gene-nutrient interactions, metabolic syndrome, insulin resistance, microarray, adipose tissue, diet intervention, insulinemic response, rye, oat, wheat",
author = "Petteri Kallio and Marjukka Kolehmainen and Laaksonen, {David E.} and Jani Kek{\"a}l{\"a}inen and Titta Salopuro and Katariina Sivenius and Leena Pulkkinen and Mykk{\"a}nen, {Hannu M.} and Leo Niskanen and Matti Uusitupa and Kaisa Poutanen",
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Kallio, P, Kolehmainen, M, Laaksonen, DE, Kekäläinen, J, Salopuro, T, Sivenius, K, Pulkkinen, L, Mykkänen, HM, Niskanen, L, Uusitupa, M & Poutanen, K 2007, 'Dietary carbohydrate modification induces alterations in gene expression in abdominal subcutaneous adipose tissue in persons with the metabolic syndrome: The FUNGENUT Study', The American journal of clinical nutrition, vol. 85, no. 5, pp. 1417-1427. https://doi.org/10.1093/ajcn/85.5.1417

Dietary carbohydrate modification induces alterations in gene expression in abdominal subcutaneous adipose tissue in persons with the metabolic syndrome: The FUNGENUT Study. / Kallio, Petteri; Kolehmainen, Marjukka (Corresponding Author); Laaksonen, David E.; Kekäläinen, Jani; Salopuro, Titta; Sivenius, Katariina; Pulkkinen, Leena; Mykkänen, Hannu M.; Niskanen, Leo; Uusitupa, Matti; Poutanen, Kaisa.

In: The American journal of clinical nutrition, Vol. 85, No. 5, 2007, p. 1417-1427.

Research output: Contribution to journalArticleScientific

TY - JOUR

T1 - Dietary carbohydrate modification induces alterations in gene expression in abdominal subcutaneous adipose tissue in persons with the metabolic syndrome: The FUNGENUT Study

AU - Kallio, Petteri

AU - Kolehmainen, Marjukka

AU - Laaksonen, David E.

AU - Kekäläinen, Jani

AU - Salopuro, Titta

AU - Sivenius, Katariina

AU - Pulkkinen, Leena

AU - Mykkänen, Hannu M.

AU - Niskanen, Leo

AU - Uusitupa, Matti

AU - Poutanen, Kaisa

PY - 2007

Y1 - 2007

N2 - Background: Diets rich in whole-grain cereals and foods with a low glycemic index may protect against type 2 diabetes, but the underlying molecular mechanisms are unknown. Objective: The main objective was to test whether 2 different carbohydrate modifications—a rye-pasta diet characterized by a low postprandial insulin response and an oat-wheat-potato diet characterized by a high postprandial insulin response—affect gene expression in subcutaneous adipose tissue (SAT) in persons with the metabolic syndrome. Design: We assessed the effect of carbohydrate modification on SAT gene expression in 47 subjects [24 men and 23 women with a mean (±SD) age of 55 ± 6 y] with the features of the metabolic syndrome in a parallel study design. The subjects had a mean (±SD) body mass index (kg/m2) of 32.1 ± 3.8 and a 2-h plasma glucose concentration of 8.0 ± 2.3 mmol/L. Adipose tissue biopsies were performed, and oral-glucose-tolerance tests and other biochemical measurements were conducted before and after the intervention. Results: We detected 71 down-regulated genes in the rye-pasta group, including genes linked to insulin signaling and apoptosis. In contrast, the 12-wk oat-wheat-potato diet up-regulated 62 genes related to stress, cytokine-chemokine–mediated immunity, and the interleukin pathway. The insulinogenic index improved after the rye-pasta diet (P = 0.004) but not after the oat-wheat-potato diet. Body weight was unchanged in both groups. Conclusions: Dietary carbohydrate modification with rye and pasta or oat, wheat, and potato differentially modulates the gene expression profile in abdominal subcutaneous adipose tissue, even in the absence of weight loss.

AB - Background: Diets rich in whole-grain cereals and foods with a low glycemic index may protect against type 2 diabetes, but the underlying molecular mechanisms are unknown. Objective: The main objective was to test whether 2 different carbohydrate modifications—a rye-pasta diet characterized by a low postprandial insulin response and an oat-wheat-potato diet characterized by a high postprandial insulin response—affect gene expression in subcutaneous adipose tissue (SAT) in persons with the metabolic syndrome. Design: We assessed the effect of carbohydrate modification on SAT gene expression in 47 subjects [24 men and 23 women with a mean (±SD) age of 55 ± 6 y] with the features of the metabolic syndrome in a parallel study design. The subjects had a mean (±SD) body mass index (kg/m2) of 32.1 ± 3.8 and a 2-h plasma glucose concentration of 8.0 ± 2.3 mmol/L. Adipose tissue biopsies were performed, and oral-glucose-tolerance tests and other biochemical measurements were conducted before and after the intervention. Results: We detected 71 down-regulated genes in the rye-pasta group, including genes linked to insulin signaling and apoptosis. In contrast, the 12-wk oat-wheat-potato diet up-regulated 62 genes related to stress, cytokine-chemokine–mediated immunity, and the interleukin pathway. The insulinogenic index improved after the rye-pasta diet (P = 0.004) but not after the oat-wheat-potato diet. Body weight was unchanged in both groups. Conclusions: Dietary carbohydrate modification with rye and pasta or oat, wheat, and potato differentially modulates the gene expression profile in abdominal subcutaneous adipose tissue, even in the absence of weight loss.

KW - Gene-nutrient interactions

KW - metabolic syndrome

KW - insulin resistance

KW - microarray

KW - adipose tissue

KW - diet intervention

KW - insulinemic response

KW - rye

KW - oat

KW - wheat

U2 - 10.1093/ajcn/85.5.1417

DO - 10.1093/ajcn/85.5.1417

M3 - Article

VL - 85

SP - 1417

EP - 1427

JO - The American journal of clinical nutrition

JF - The American journal of clinical nutrition

SN - 0002-9165

IS - 5

ER -