Dietary Oat Bran Increases Some Proinflammatory Polyunsaturated Fatty-Acid Oxidation Products and Reduces Anti-Inflammatory Products in Apolipoprotein E−/− Mice

Jetty Chung Yung Lee (Corresponding Author), Dalal Samir AlGhawas, Kaisa Poutanen, Kin Sum Leung, Camille Oger, Jean Marie Galano, Thierry Durand, Hani El-Nezami

Research output: Contribution to journalArticleScientificpeer-review

1 Citation (Scopus)

Abstract

Oat bran is suggested to attenuate atherosclerotic conditions by regulating dyslipidemia, endothelial function, and oxidative damage. Through the measurement of oxidized polyunsaturated fatty acid (PUFA), oxidative stress, and inflammation status in liver and heart tissues of apolipoprotein E−/− (ApoE−/−), mice fed with high fat diet (HFD) or HFD with oat bran (HFD + Oat) were investigated. Using liquid chromatography tandem mass spectrometry (LC–MS/MS), PUFA and over 40 types of its oxidized products were assessed. The HFD + Oat group had augmented adrenic acid (ADA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) and suppressed n-3 docosapentaenoic acid levels in the liver tissues compared to the HFD group. Arachidonic acid (ARA) and α-linolenic acid (ALA) levels were elevated and ADA was suppressed in the heart tissues of the HFD + Oat group compared to the HFD group. Furthermore, enzymatically mediated oxidized ARA product levels (9-, 11- and 20-HETE [hydroxyeicosatetraenoic acid], and PGF) were augmented and those of the oxidized DHA products (4-, 7-, 10-, 11-, 13-, and 14-HDHA [hydroxy-docosahexaenoic acid]) were reduced in the liver tissues of the HFD + Oat group. It also increased 17-F2t-dihomo-isoprostane and 7-F2t-dihomo-isofuran derived from nonenzymatic oxidation of ADA in the heart and liver tissues, and those from ALA namely 16-F1t-phytoprostane and 16(RS)-13-epi-STΔ14-9-phytofuran. Our study showed oat bran to be a weak antioxidant and lacked anti-inflammatory properties in atherosclerotic mice. Elevation of oxidized PUFA products that are potentially proinflammatory and vasoconstrictors (HETE, PGF) with simultaneous reduction of those that are anti-inflammatory (HDHA) may not be desirable in the pathogenesis of atherosclerosis.

Original languageEnglish
Pages (from-to)785-796
Number of pages12
JournalLipids
Volume53
Issue number8
DOIs
Publication statusPublished - 22 Oct 2018
MoE publication typeNot Eligible

Fingerprint

High Fat Diet
Apolipoproteins E
Nutrition
Unsaturated Fatty Acids
Anti-Inflammatory Agents
Fats
Oxidation
Liver
Tissue
Docosahexaenoic Acids
Hydroxyeicosatetraenoic Acids
Dinoprost
alpha-Linolenic Acid
Arachidonic Acid
Isoprostanes
Hydroxy Acids
Oxidative stress
Eicosapentaenoic Acid
Avena
Liquid chromatography

Keywords

  • Atherosclerosis
  • HDHA
  • HETE
  • Isoprostanes
  • Oat bran
  • Polyunsaturated fatty acid

Cite this

Lee, Jetty Chung Yung ; AlGhawas, Dalal Samir ; Poutanen, Kaisa ; Leung, Kin Sum ; Oger, Camille ; Galano, Jean Marie ; Durand, Thierry ; El-Nezami, Hani. / Dietary Oat Bran Increases Some Proinflammatory Polyunsaturated Fatty-Acid Oxidation Products and Reduces Anti-Inflammatory Products in Apolipoprotein E−/− Mice. In: Lipids. 2018 ; Vol. 53, No. 8. pp. 785-796.
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abstract = "Oat bran is suggested to attenuate atherosclerotic conditions by regulating dyslipidemia, endothelial function, and oxidative damage. Through the measurement of oxidized polyunsaturated fatty acid (PUFA), oxidative stress, and inflammation status in liver and heart tissues of apolipoprotein E−/− (ApoE−/−), mice fed with high fat diet (HFD) or HFD with oat bran (HFD + Oat) were investigated. Using liquid chromatography tandem mass spectrometry (LC–MS/MS), PUFA and over 40 types of its oxidized products were assessed. The HFD + Oat group had augmented adrenic acid (ADA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) and suppressed n-3 docosapentaenoic acid levels in the liver tissues compared to the HFD group. Arachidonic acid (ARA) and α-linolenic acid (ALA) levels were elevated and ADA was suppressed in the heart tissues of the HFD + Oat group compared to the HFD group. Furthermore, enzymatically mediated oxidized ARA product levels (9-, 11- and 20-HETE [hydroxyeicosatetraenoic acid], and PGF2α) were augmented and those of the oxidized DHA products (4-, 7-, 10-, 11-, 13-, and 14-HDHA [hydroxy-docosahexaenoic acid]) were reduced in the liver tissues of the HFD + Oat group. It also increased 17-F2t-dihomo-isoprostane and 7-F2t-dihomo-isofuran derived from nonenzymatic oxidation of ADA in the heart and liver tissues, and those from ALA namely 16-F1t-phytoprostane and 16(RS)-13-epi-STΔ14-9-phytofuran. Our study showed oat bran to be a weak antioxidant and lacked anti-inflammatory properties in atherosclerotic mice. Elevation of oxidized PUFA products that are potentially proinflammatory and vasoconstrictors (HETE, PGF2α) with simultaneous reduction of those that are anti-inflammatory (HDHA) may not be desirable in the pathogenesis of atherosclerosis.",
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Dietary Oat Bran Increases Some Proinflammatory Polyunsaturated Fatty-Acid Oxidation Products and Reduces Anti-Inflammatory Products in Apolipoprotein E−/− Mice. / Lee, Jetty Chung Yung (Corresponding Author); AlGhawas, Dalal Samir; Poutanen, Kaisa; Leung, Kin Sum; Oger, Camille; Galano, Jean Marie; Durand, Thierry; El-Nezami, Hani.

In: Lipids, Vol. 53, No. 8, 22.10.2018, p. 785-796.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Dietary Oat Bran Increases Some Proinflammatory Polyunsaturated Fatty-Acid Oxidation Products and Reduces Anti-Inflammatory Products in Apolipoprotein E−/− Mice

AU - Lee, Jetty Chung Yung

AU - AlGhawas, Dalal Samir

AU - Poutanen, Kaisa

AU - Leung, Kin Sum

AU - Oger, Camille

AU - Galano, Jean Marie

AU - Durand, Thierry

AU - El-Nezami, Hani

PY - 2018/10/22

Y1 - 2018/10/22

N2 - Oat bran is suggested to attenuate atherosclerotic conditions by regulating dyslipidemia, endothelial function, and oxidative damage. Through the measurement of oxidized polyunsaturated fatty acid (PUFA), oxidative stress, and inflammation status in liver and heart tissues of apolipoprotein E−/− (ApoE−/−), mice fed with high fat diet (HFD) or HFD with oat bran (HFD + Oat) were investigated. Using liquid chromatography tandem mass spectrometry (LC–MS/MS), PUFA and over 40 types of its oxidized products were assessed. The HFD + Oat group had augmented adrenic acid (ADA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) and suppressed n-3 docosapentaenoic acid levels in the liver tissues compared to the HFD group. Arachidonic acid (ARA) and α-linolenic acid (ALA) levels were elevated and ADA was suppressed in the heart tissues of the HFD + Oat group compared to the HFD group. Furthermore, enzymatically mediated oxidized ARA product levels (9-, 11- and 20-HETE [hydroxyeicosatetraenoic acid], and PGF2α) were augmented and those of the oxidized DHA products (4-, 7-, 10-, 11-, 13-, and 14-HDHA [hydroxy-docosahexaenoic acid]) were reduced in the liver tissues of the HFD + Oat group. It also increased 17-F2t-dihomo-isoprostane and 7-F2t-dihomo-isofuran derived from nonenzymatic oxidation of ADA in the heart and liver tissues, and those from ALA namely 16-F1t-phytoprostane and 16(RS)-13-epi-STΔ14-9-phytofuran. Our study showed oat bran to be a weak antioxidant and lacked anti-inflammatory properties in atherosclerotic mice. Elevation of oxidized PUFA products that are potentially proinflammatory and vasoconstrictors (HETE, PGF2α) with simultaneous reduction of those that are anti-inflammatory (HDHA) may not be desirable in the pathogenesis of atherosclerosis.

AB - Oat bran is suggested to attenuate atherosclerotic conditions by regulating dyslipidemia, endothelial function, and oxidative damage. Through the measurement of oxidized polyunsaturated fatty acid (PUFA), oxidative stress, and inflammation status in liver and heart tissues of apolipoprotein E−/− (ApoE−/−), mice fed with high fat diet (HFD) or HFD with oat bran (HFD + Oat) were investigated. Using liquid chromatography tandem mass spectrometry (LC–MS/MS), PUFA and over 40 types of its oxidized products were assessed. The HFD + Oat group had augmented adrenic acid (ADA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) and suppressed n-3 docosapentaenoic acid levels in the liver tissues compared to the HFD group. Arachidonic acid (ARA) and α-linolenic acid (ALA) levels were elevated and ADA was suppressed in the heart tissues of the HFD + Oat group compared to the HFD group. Furthermore, enzymatically mediated oxidized ARA product levels (9-, 11- and 20-HETE [hydroxyeicosatetraenoic acid], and PGF2α) were augmented and those of the oxidized DHA products (4-, 7-, 10-, 11-, 13-, and 14-HDHA [hydroxy-docosahexaenoic acid]) were reduced in the liver tissues of the HFD + Oat group. It also increased 17-F2t-dihomo-isoprostane and 7-F2t-dihomo-isofuran derived from nonenzymatic oxidation of ADA in the heart and liver tissues, and those from ALA namely 16-F1t-phytoprostane and 16(RS)-13-epi-STΔ14-9-phytofuran. Our study showed oat bran to be a weak antioxidant and lacked anti-inflammatory properties in atherosclerotic mice. Elevation of oxidized PUFA products that are potentially proinflammatory and vasoconstrictors (HETE, PGF2α) with simultaneous reduction of those that are anti-inflammatory (HDHA) may not be desirable in the pathogenesis of atherosclerosis.

KW - Atherosclerosis

KW - HDHA

KW - HETE

KW - Isoprostanes

KW - Oat bran

KW - Polyunsaturated fatty acid

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