Diethyl phthalate in compost

Ecotoxicological effects and response of the microbial community

Anu Kapanen, J. R. Stephen, J. Brüggemann, A. Kiviranta, D. C. White, Merja Itävaara

Research output: Contribution to journalArticleScientificpeer-review

43 Citations (Scopus)

Abstract

There is a great need to understand the environmental impacts of organic pollutants on soil health. Phthalates are widely used in consumables and can be found extensively. We studied the toxicity of diethyl phthalate (DEP), spiked in a compost plant growth substrate, by means of the acute toxicity Flash test and on the basis of the germination and plant growth of radish seedlings. The response of the microbial community to DEP in the growth substrate was studied by PCR-DGGE (denaturing gradient gel electrophoresis). In the acute toxicity test, DEP was found to be less toxic as a pure compound than when mixed with the compost mixture. This suggests the synergistic effect of unknown toxic compounds or the release of compounds due to DEP addition. The same DEP concentration level in compost substrate induced toxic response in both plant test and microbial community analysis. The diversity of the major microbial community was reduced from a broad community to only 10 major species at toxic concentrations of DEP. Several of the identified microbial species are known to be able to degrade phthalates, which means that the suppression of other microbial species might be due to the substrate availability and toxicity. The major species identified included Sphingomonas sp., Pseudomonas sp., Actinomycetes sp.
Original languageEnglish
Pages (from-to)2201-2209
JournalChemosphere
Volume67
Issue number11
DOIs
Publication statusPublished - 2007
MoE publication typeA1 Journal article-refereed

Fingerprint

phthalate
compost
Toxicity
microbial community
Soil
Poisons
Substrates
Acute Toxicity Tests
toxicity
substrate
Organic pollutants
Soil Pollutants
Growth
Electrophoresis
Sphingomonas
Environmental impact
Raphanus
Denaturing Gradient Gel Electrophoresis
Gels
Actinobacteria

Keywords

  • Diethyl phthalate
  • Phytotoxicity
  • Compost
  • Microbial community structure
  • Biotest

Cite this

Kapanen, A., Stephen, J. R., Brüggemann, J., Kiviranta, A., White, D. C., & Itävaara, M. (2007). Diethyl phthalate in compost: Ecotoxicological effects and response of the microbial community. Chemosphere, 67(11), 2201-2209. https://doi.org/10.1016/j.chemosphere.2006.12.023
Kapanen, Anu ; Stephen, J. R. ; Brüggemann, J. ; Kiviranta, A. ; White, D. C. ; Itävaara, Merja. / Diethyl phthalate in compost : Ecotoxicological effects and response of the microbial community. In: Chemosphere. 2007 ; Vol. 67, No. 11. pp. 2201-2209.
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Kapanen, A, Stephen, JR, Brüggemann, J, Kiviranta, A, White, DC & Itävaara, M 2007, 'Diethyl phthalate in compost: Ecotoxicological effects and response of the microbial community', Chemosphere, vol. 67, no. 11, pp. 2201-2209. https://doi.org/10.1016/j.chemosphere.2006.12.023

Diethyl phthalate in compost : Ecotoxicological effects and response of the microbial community. / Kapanen, Anu; Stephen, J. R.; Brüggemann, J.; Kiviranta, A.; White, D. C.; Itävaara, Merja.

In: Chemosphere, Vol. 67, No. 11, 2007, p. 2201-2209.

Research output: Contribution to journalArticleScientificpeer-review

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T2 - Ecotoxicological effects and response of the microbial community

AU - Kapanen, Anu

AU - Stephen, J. R.

AU - Brüggemann, J.

AU - Kiviranta, A.

AU - White, D. C.

AU - Itävaara, Merja

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AB - There is a great need to understand the environmental impacts of organic pollutants on soil health. Phthalates are widely used in consumables and can be found extensively. We studied the toxicity of diethyl phthalate (DEP), spiked in a compost plant growth substrate, by means of the acute toxicity Flash test and on the basis of the germination and plant growth of radish seedlings. The response of the microbial community to DEP in the growth substrate was studied by PCR-DGGE (denaturing gradient gel electrophoresis). In the acute toxicity test, DEP was found to be less toxic as a pure compound than when mixed with the compost mixture. This suggests the synergistic effect of unknown toxic compounds or the release of compounds due to DEP addition. The same DEP concentration level in compost substrate induced toxic response in both plant test and microbial community analysis. The diversity of the major microbial community was reduced from a broad community to only 10 major species at toxic concentrations of DEP. Several of the identified microbial species are known to be able to degrade phthalates, which means that the suppression of other microbial species might be due to the substrate availability and toxicity. The major species identified included Sphingomonas sp., Pseudomonas sp., Actinomycetes sp.

KW - Diethyl phthalate

KW - Phytotoxicity

KW - Compost

KW - Microbial community structure

KW - Biotest

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