Differential expression of the vegetative and spore-bound hydrophobins of Trichoderma reesei cloning and characterization of the Hfb2 gene

Tiina Nakari-Setälä (Corresponding Author), Nina Aro, Marja Ilmen, Gaston Munoz, Nisse Kalkkinen, Merja Penttilä

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Abstract

The hfb2 gene encoding the hydrophobin HFBII of the filamentous fungus Trichoderma reesei was isolated by heterologous hybridization using the vegetative hydrophobin I, hfb1, gene of T. reesei as a probe. The hfb2 gene codes for a typical fungal secreted hydrophobin of 71 amino acids containing eight cysteine residues. The amino acid similarity towards HFBI is 69%. The HFBII protein was isolated from the fungal spores by extraction with trifluoroacetic acid/acetonitrile solution, and by bubbling from the lactose‐based culture medium. Expression of the hfb1 and hfb2 genes is divergent, hfb1 expression was only observed in vegetative cultures on glucose‐containing and sorbitol‐containing media. It was not expressed on media containing complex plant polysaccharides, cellulose, xylan, cellobiose or lactose, whereas hfb2 was highly expressed in vegetative cultures on these media. Expression of hfb2 was also strongly induced by N and C starvation, by light and in conidiating cultures.
Original languageEnglish
Pages (from-to)415-423
JournalEuropean Journal of Biochemistry
Volume248
Issue number2
DOIs
Publication statusPublished - 1997
MoE publication typeA1 Journal article-refereed

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Trichoderma
Cloning
Spores
Organism Cloning
Genes
Culture Media
Cellobiose
Amino Acids
Xylans
Trifluoroacetic Acid
Gene encoding
Lactose
Fungi
Cellulose
Fungal Spores
Polysaccharides
Cysteine
Starvation
Light
Proteins

Cite this

@article{5b8a2529e138499b9212e29c8b1b91ed,
title = "Differential expression of the vegetative and spore-bound hydrophobins of Trichoderma reesei cloning and characterization of the Hfb2 gene",
abstract = "The hfb2 gene encoding the hydrophobin HFBII of the filamentous fungus Trichoderma reesei was isolated by heterologous hybridization using the vegetative hydrophobin I, hfb1, gene of T. reesei as a probe. The hfb2 gene codes for a typical fungal secreted hydrophobin of 71 amino acids containing eight cysteine residues. The amino acid similarity towards HFBI is 69{\%}. The HFBII protein was isolated from the fungal spores by extraction with trifluoroacetic acid/acetonitrile solution, and by bubbling from the lactose‐based culture medium. Expression of the hfb1 and hfb2 genes is divergent, hfb1 expression was only observed in vegetative cultures on glucose‐containing and sorbitol‐containing media. It was not expressed on media containing complex plant polysaccharides, cellulose, xylan, cellobiose or lactose, whereas hfb2 was highly expressed in vegetative cultures on these media. Expression of hfb2 was also strongly induced by N and C starvation, by light and in conidiating cultures.",
author = "Tiina Nakari-Set{\"a}l{\"a} and Nina Aro and Marja Ilmen and Gaston Munoz and Nisse Kalkkinen and Merja Penttil{\"a}",
year = "1997",
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language = "English",
volume = "248",
pages = "415--423",
journal = "FEBS Journal",
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TY - JOUR

T1 - Differential expression of the vegetative and spore-bound hydrophobins of Trichoderma reesei cloning and characterization of the Hfb2 gene

AU - Nakari-Setälä, Tiina

AU - Aro, Nina

AU - Ilmen, Marja

AU - Munoz, Gaston

AU - Kalkkinen, Nisse

AU - Penttilä, Merja

PY - 1997

Y1 - 1997

N2 - The hfb2 gene encoding the hydrophobin HFBII of the filamentous fungus Trichoderma reesei was isolated by heterologous hybridization using the vegetative hydrophobin I, hfb1, gene of T. reesei as a probe. The hfb2 gene codes for a typical fungal secreted hydrophobin of 71 amino acids containing eight cysteine residues. The amino acid similarity towards HFBI is 69%. The HFBII protein was isolated from the fungal spores by extraction with trifluoroacetic acid/acetonitrile solution, and by bubbling from the lactose‐based culture medium. Expression of the hfb1 and hfb2 genes is divergent, hfb1 expression was only observed in vegetative cultures on glucose‐containing and sorbitol‐containing media. It was not expressed on media containing complex plant polysaccharides, cellulose, xylan, cellobiose or lactose, whereas hfb2 was highly expressed in vegetative cultures on these media. Expression of hfb2 was also strongly induced by N and C starvation, by light and in conidiating cultures.

AB - The hfb2 gene encoding the hydrophobin HFBII of the filamentous fungus Trichoderma reesei was isolated by heterologous hybridization using the vegetative hydrophobin I, hfb1, gene of T. reesei as a probe. The hfb2 gene codes for a typical fungal secreted hydrophobin of 71 amino acids containing eight cysteine residues. The amino acid similarity towards HFBI is 69%. The HFBII protein was isolated from the fungal spores by extraction with trifluoroacetic acid/acetonitrile solution, and by bubbling from the lactose‐based culture medium. Expression of the hfb1 and hfb2 genes is divergent, hfb1 expression was only observed in vegetative cultures on glucose‐containing and sorbitol‐containing media. It was not expressed on media containing complex plant polysaccharides, cellulose, xylan, cellobiose or lactose, whereas hfb2 was highly expressed in vegetative cultures on these media. Expression of hfb2 was also strongly induced by N and C starvation, by light and in conidiating cultures.

U2 - 10.1111/j.1432-1033.1997.00415.x

DO - 10.1111/j.1432-1033.1997.00415.x

M3 - Article

VL - 248

SP - 415

EP - 423

JO - FEBS Journal

JF - FEBS Journal

SN - 1742-464X

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