Differential expression of the vegetative and spore-bound hydrophobins of Trichoderma reesei

Cloning and characterization of the hfb2 gene

Tiina Nakari-Setälä, Nina Aro, Marja Ilmén, Gastón Muñoz, Nisse Kalkkinen, Merja Penttilä

Research output: Book/ReportReportProfessional

Abstract

The hfb2 gene encoding the hydrophobin HFBII of the filamentous fungus Trichoderma reesei was isolated by heterologous hybridization using the vegetative hydrophobin I, hfb1, gene of T. reesei as a probe. The hfb2 gene codes for a typical fungal secreted hydrophobin of 71 amino acids containing eight cysteine residues. The amino acid similarity towards HFBI is 69%. The HFBII protein was isolated from the fungal spores by extraction with trifluoroacetic acid acetonitrile solution. Expression of the hfb1 and hfb2 genes is divergent, hfb1 expression was only observed in vegetative cultures on glucose- and sorbitol-containing media. It was not expressed on media containing complex plant polysaccharides, cellulose, xylan, cellobiose or lactose, whereas hfb2 was highly expressed in vegetative cultures on these media. Expression of hfb2 was also strongly induced by N- and C-starvation, by light and in conidiating cultures.

Original languageEnglish
PublisherVTT Technical Research Centre of Finland
Number of pages15
Publication statusPublished - 1 Dec 1996
MoE publication typeD4 Published development or research report or study

Publication series

NameVTT Publications
PublisherVTT
No.254
ISSN (Print)1235-0621

Fingerprint

Cloning
Genes
Amino acids
Trifluoroacetic acid
Gene encoding
Polysaccharides
Fungi
Acetonitrile
Glucose
Cellulose
Proteins

Keywords

  • Carbon/nitrogen regulation
  • Filamentous fungus
  • hfb1
  • Light induction
  • Spore-protein
  • Starvation
  • Transcriptional regulation

Cite this

@book{76335a3e381a4a8aa74d9261ff412555,
title = "Differential expression of the vegetative and spore-bound hydrophobins of Trichoderma reesei: Cloning and characterization of the hfb2 gene",
abstract = "The hfb2 gene encoding the hydrophobin HFBII of the filamentous fungus Trichoderma reesei was isolated by heterologous hybridization using the vegetative hydrophobin I, hfb1, gene of T. reesei as a probe. The hfb2 gene codes for a typical fungal secreted hydrophobin of 71 amino acids containing eight cysteine residues. The amino acid similarity towards HFBI is 69{\%}. The HFBII protein was isolated from the fungal spores by extraction with trifluoroacetic acid acetonitrile solution. Expression of the hfb1 and hfb2 genes is divergent, hfb1 expression was only observed in vegetative cultures on glucose- and sorbitol-containing media. It was not expressed on media containing complex plant polysaccharides, cellulose, xylan, cellobiose or lactose, whereas hfb2 was highly expressed in vegetative cultures on these media. Expression of hfb2 was also strongly induced by N- and C-starvation, by light and in conidiating cultures.",
keywords = "Carbon/nitrogen regulation, Filamentous fungus, hfb1, Light induction, Spore-protein, Starvation, Transcriptional regulation",
author = "Tiina Nakari-Set{\"a}l{\"a} and Nina Aro and Marja Ilm{\'e}n and Gast{\'o}n Mu{\~n}oz and Nisse Kalkkinen and Merja Penttil{\"a}",
year = "1996",
month = "12",
day = "1",
language = "English",
series = "VTT Publications",
publisher = "VTT Technical Research Centre of Finland",
number = "254",
address = "Finland",

}

Differential expression of the vegetative and spore-bound hydrophobins of Trichoderma reesei : Cloning and characterization of the hfb2 gene. / Nakari-Setälä, Tiina; Aro, Nina; Ilmén, Marja; Muñoz, Gastón; Kalkkinen, Nisse; Penttilä, Merja.

VTT Technical Research Centre of Finland, 1996. 15 p. (VTT Publications; No. 254).

Research output: Book/ReportReportProfessional

TY - BOOK

T1 - Differential expression of the vegetative and spore-bound hydrophobins of Trichoderma reesei

T2 - Cloning and characterization of the hfb2 gene

AU - Nakari-Setälä, Tiina

AU - Aro, Nina

AU - Ilmén, Marja

AU - Muñoz, Gastón

AU - Kalkkinen, Nisse

AU - Penttilä, Merja

PY - 1996/12/1

Y1 - 1996/12/1

N2 - The hfb2 gene encoding the hydrophobin HFBII of the filamentous fungus Trichoderma reesei was isolated by heterologous hybridization using the vegetative hydrophobin I, hfb1, gene of T. reesei as a probe. The hfb2 gene codes for a typical fungal secreted hydrophobin of 71 amino acids containing eight cysteine residues. The amino acid similarity towards HFBI is 69%. The HFBII protein was isolated from the fungal spores by extraction with trifluoroacetic acid acetonitrile solution. Expression of the hfb1 and hfb2 genes is divergent, hfb1 expression was only observed in vegetative cultures on glucose- and sorbitol-containing media. It was not expressed on media containing complex plant polysaccharides, cellulose, xylan, cellobiose or lactose, whereas hfb2 was highly expressed in vegetative cultures on these media. Expression of hfb2 was also strongly induced by N- and C-starvation, by light and in conidiating cultures.

AB - The hfb2 gene encoding the hydrophobin HFBII of the filamentous fungus Trichoderma reesei was isolated by heterologous hybridization using the vegetative hydrophobin I, hfb1, gene of T. reesei as a probe. The hfb2 gene codes for a typical fungal secreted hydrophobin of 71 amino acids containing eight cysteine residues. The amino acid similarity towards HFBI is 69%. The HFBII protein was isolated from the fungal spores by extraction with trifluoroacetic acid acetonitrile solution. Expression of the hfb1 and hfb2 genes is divergent, hfb1 expression was only observed in vegetative cultures on glucose- and sorbitol-containing media. It was not expressed on media containing complex plant polysaccharides, cellulose, xylan, cellobiose or lactose, whereas hfb2 was highly expressed in vegetative cultures on these media. Expression of hfb2 was also strongly induced by N- and C-starvation, by light and in conidiating cultures.

KW - Carbon/nitrogen regulation

KW - Filamentous fungus

KW - hfb1

KW - Light induction

KW - Spore-protein

KW - Starvation

KW - Transcriptional regulation

UR - http://www.scopus.com/inward/record.url?scp=4243080159&partnerID=8YFLogxK

M3 - Report

T3 - VTT Publications

BT - Differential expression of the vegetative and spore-bound hydrophobins of Trichoderma reesei

PB - VTT Technical Research Centre of Finland

ER -