Abstract
The aim of this study was to use whole cell catalysts as tools for
modification of selected resin acids in order to obtain value-added
functional derivatives. The enzymatic bioconversion capacities of two
plant species were tested towards dehydroabietic acid. Dehydroabietic
acid (DHA) is an abundant resin acid in conifers, representing a natural
wood protectant. It is also one of the constituents found in
by-products of the kraft chemical pulping industry. DHA was fed to
tobacco (Nicotiana tabacum) and Madagascar periwinkle (Catharanthus roseus)
plant cell and tissue cultures and bioconversion product formation was
monitored using NMR analysis. Both plant species took up DHA from
culture medium, and various types of typical detoxification processes
occurred in both cultures. In addition, diverse responses to DHA
treatment were observed, including differences in uptake kinetics,
chemical modification of added substrate and changes in overall
metabolism of the cells. Interestingly, Catharanthus roseus, a
host species for pharmaceutically valuable terpenoid indole alkaloids,
exhibited a very different bioconversion pattern for exogenously applied
DHA than tobacco, which does not possess a terpenoid indole pathway. In
tobacco, DHA is readily glycosylated in the carbonyl group, whereas in
periwinkle it is proposed that a cytochrome P450-catalyzed enzymatic
detoxification reaction takes place before the formation of glycosylated
product.
| Original language | English |
|---|---|
| Pages (from-to) | 287-294 |
| Journal | Journal of Biotechnology |
| Volume | 157 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2012 |
| MoE publication type | A1 Journal article-refereed |
Keywords
- Biotransformation
- Nicotiana tabacum
- Catharanthus roseus
- Dehydroabietic acid
- Cell cultures
- Hairy roots