Digestion of single crystals of mannan I by an endo-mannanase from Trichoderma reesei

Elisabetta Sabini, Keith Wilson, Matti Siika-aho, Claire Boisset, Henri Chanzy (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

7 Citations (Scopus)

Abstract

The enzymatic degradation of single crystals of mannan I with the catalytic core domain of a β‐mannanase (EC 3.2.1.78 or Man5A) from Trichoderma reesei was investigated by transmission electron microscopy and electron diffraction. The enzyme attack took place at the edge of the crystals and progressed towards their centres. Quite remarkably the crystalline integrity of the crystals was preserved almost to the end of the digestion process. This behaviour is consistent with an endo‐mechanism, where the enzyme interacts with the accessible mannan chains located at the crystal periphery and cleaves one mannan molecule at a time. The endo mode of digestion of the crystals was confirmed by an analysis of the soluble degradation products.

Original languageEnglish
Pages (from-to)2340 - 2344
Number of pages5
JournalEuropean Journal of Biochemistry
Volume267
Issue number8
DOIs
Publication statusPublished - 2000
MoE publication typeA1 Journal article-refereed

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Mannans
Trichoderma
Digestion
Single crystals
Crystals
Catalytic Domain
Enzymes
Transmission Electron Microscopy
Degradation
Electron diffraction
Electrons
Crystalline materials
Transmission electron microscopy
Molecules

Cite this

Sabini, Elisabetta ; Wilson, Keith ; Siika-aho, Matti ; Boisset, Claire ; Chanzy, Henri. / Digestion of single crystals of mannan I by an endo-mannanase from Trichoderma reesei. In: European Journal of Biochemistry. 2000 ; Vol. 267, No. 8. pp. 2340 - 2344.
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Digestion of single crystals of mannan I by an endo-mannanase from Trichoderma reesei. / Sabini, Elisabetta; Wilson, Keith; Siika-aho, Matti; Boisset, Claire; Chanzy, Henri (Corresponding Author).

In: European Journal of Biochemistry, Vol. 267, No. 8, 2000, p. 2340 - 2344.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Digestion of single crystals of mannan I by an endo-mannanase from Trichoderma reesei

AU - Sabini, Elisabetta

AU - Wilson, Keith

AU - Siika-aho, Matti

AU - Boisset, Claire

AU - Chanzy, Henri

PY - 2000

Y1 - 2000

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AB - The enzymatic degradation of single crystals of mannan I with the catalytic core domain of a β‐mannanase (EC 3.2.1.78 or Man5A) from Trichoderma reesei was investigated by transmission electron microscopy and electron diffraction. The enzyme attack took place at the edge of the crystals and progressed towards their centres. Quite remarkably the crystalline integrity of the crystals was preserved almost to the end of the digestion process. This behaviour is consistent with an endo‐mechanism, where the enzyme interacts with the accessible mannan chains located at the crystal periphery and cleaves one mannan molecule at a time. The endo mode of digestion of the crystals was confirmed by an analysis of the soluble degradation products.

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DO - 10.1046/j.1432-1327.2000.01242.x

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