Direct identification of hydrophobins and their processing in Trichoderma using intact-cell MALDI-TOF MS

Torsten Neuhof, Ralf Dieckmann, Irina S. Druzhinina, Christian P. Kubicek, Tiina Nakari-Setälä, Merja Penttilä, Hans von Döhren

    Research output: Contribution to journalArticleScientificpeer-review

    40 Citations (Scopus)

    Abstract

    Intact-cell MS (ICMS) was applied for the direct detection of hydrophobins in various species and strains of Hypocrea/Trichoderma. In both mycelia and spores, dominating peaks were identified as hydrophobins by detecting mass shifts of 8 Da of reduced and unreduced forms, the analysis of knockout mutants, and comparison with protein databases. Strain-specific processing was observed in the case of Hypocrea jecorina (anamorph Trichoderma reesei). An analysis of 32 strains comprising 29 different species of Trichoderma and Hypocrea showed hydrophobin patterns that were specific at both at the species and isolate (subspecies) levels. The method therefore permits rapid and direct detection of hydrophobin class II compositions and may also provide a means to identify Trichoderma (and other fungal) species and strains from microgram amounts of biomass without prior cultivation.
    Original languageEnglish
    Pages (from-to)841-852
    JournalFEBS Journal
    Volume274
    Issue number3
    DOIs
    Publication statusPublished - 2007
    MoE publication typeA1 Journal article-refereed

    Keywords

    • fungal biomarker
    • hydrophobins
    • intact-cell MS
    • MALDI-TOF MS
    • Trichoderma

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