Direct identification of hydrophobins and their processing in Trichoderma using intact-cell MALDI-TOF MS

Torsten Neuhof, Ralf Dieckmann, Irina S. Druzhinina, Christian P. Kubicek, Tiina Nakari-Setälä, Merja Penttilä, Hans von Döhren

Research output: Contribution to journalArticleScientificpeer-review

37 Citations (Scopus)

Abstract

Intact-cell MS (ICMS) was applied for the direct detection of hydrophobins in various species and strains of Hypocrea/Trichoderma. In both mycelia and spores, dominating peaks were identified as hydrophobins by detecting mass shifts of 8 Da of reduced and unreduced forms, the analysis of knockout mutants, and comparison with protein databases. Strain-specific processing was observed in the case of Hypocrea jecorina (anamorph Trichoderma reesei). An analysis of 32 strains comprising 29 different species of Trichoderma and Hypocrea showed hydrophobin patterns that were specific at both at the species and isolate (subspecies) levels. The method therefore permits rapid and direct detection of hydrophobin class II compositions and may also provide a means to identify Trichoderma (and other fungal) species and strains from microgram amounts of biomass without prior cultivation.
Original languageEnglish
Pages (from-to)841-852
JournalFEBS Journal
Volume274
Issue number3
DOIs
Publication statusPublished - 2007
MoE publication typeA1 Journal article-refereed

Fingerprint

Trichoderma
Hypocrea
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Processing
Protein Databases
Mycelium
Biomass
Spores
Chemical analysis
Proteins

Keywords

  • fungal biomarker
  • hydrophobins
  • intact-cell MS
  • MALDI-TOF MS
  • Trichoderma

Cite this

Neuhof, Torsten ; Dieckmann, Ralf ; Druzhinina, Irina S. ; Kubicek, Christian P. ; Nakari-Setälä, Tiina ; Penttilä, Merja ; von Döhren, Hans. / Direct identification of hydrophobins and their processing in Trichoderma using intact-cell MALDI-TOF MS. In: FEBS Journal. 2007 ; Vol. 274, No. 3. pp. 841-852.
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abstract = "Intact-cell MS (ICMS) was applied for the direct detection of hydrophobins in various species and strains of Hypocrea/Trichoderma. In both mycelia and spores, dominating peaks were identified as hydrophobins by detecting mass shifts of 8 Da of reduced and unreduced forms, the analysis of knockout mutants, and comparison with protein databases. Strain-specific processing was observed in the case of Hypocrea jecorina (anamorph Trichoderma reesei). An analysis of 32 strains comprising 29 different species of Trichoderma and Hypocrea showed hydrophobin patterns that were specific at both at the species and isolate (subspecies) levels. The method therefore permits rapid and direct detection of hydrophobin class II compositions and may also provide a means to identify Trichoderma (and other fungal) species and strains from microgram amounts of biomass without prior cultivation.",
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Direct identification of hydrophobins and their processing in Trichoderma using intact-cell MALDI-TOF MS. / Neuhof, Torsten; Dieckmann, Ralf; Druzhinina, Irina S.; Kubicek, Christian P.; Nakari-Setälä, Tiina; Penttilä, Merja; von Döhren, Hans.

In: FEBS Journal, Vol. 274, No. 3, 2007, p. 841-852.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Direct identification of hydrophobins and their processing in Trichoderma using intact-cell MALDI-TOF MS

AU - Neuhof, Torsten

AU - Dieckmann, Ralf

AU - Druzhinina, Irina S.

AU - Kubicek, Christian P.

AU - Nakari-Setälä, Tiina

AU - Penttilä, Merja

AU - von Döhren, Hans

PY - 2007

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AB - Intact-cell MS (ICMS) was applied for the direct detection of hydrophobins in various species and strains of Hypocrea/Trichoderma. In both mycelia and spores, dominating peaks were identified as hydrophobins by detecting mass shifts of 8 Da of reduced and unreduced forms, the analysis of knockout mutants, and comparison with protein databases. Strain-specific processing was observed in the case of Hypocrea jecorina (anamorph Trichoderma reesei). An analysis of 32 strains comprising 29 different species of Trichoderma and Hypocrea showed hydrophobin patterns that were specific at both at the species and isolate (subspecies) levels. The method therefore permits rapid and direct detection of hydrophobin class II compositions and may also provide a means to identify Trichoderma (and other fungal) species and strains from microgram amounts of biomass without prior cultivation.

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KW - MALDI-TOF MS

KW - Trichoderma

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