Discovery of novel secreted fungal sulfhydryl oxidases with a plate test screen

O. Nivala, Maija Mattinen (Corresponding Author), G. Faccio, Johanna Buchert, Kristiina Kruus

Research output: Contribution to journalArticleScientificpeer-review

2 Citations (Scopus)

Abstract

Sulfhydryl oxidases (SOX) are FAD-dependent enzymes capable of oxidising free thiol groups and forming disulphide bonds. Although the quantity of scientific papers and suggested applications for SOX is constantly increasing, only a limited number of microbial SOX have been reported and are commercially available. Hence, the aim of this study was to develop a fast and reliable qualitative plate test for screening novel secreted fungal SOX. The screening was based on the Ellman's reagent, i.e. 5,5′-dithiobis[2-nitrobenzoic acid]. Altogether, 32 fungal strains from an in-house culture collection were screened. A total of 13 SOX-producing strains were found positive in the plate test screen. The novel SOX producers were Aspergillus tubingensis, Chaetomium globusum, Melanocarpus albomyces, Penicillium aurantiogriseum, Penicillium funiculosum, Coniophora puteana and Trametes hirsuta. Six of the discovered SOX were partially characterised by determination of isoelectric point, pH optimum and substrate specificity. A. tubingensis was identified as the most efficient novel SOX producer.
Original languageEnglish
Pages (from-to)9429-9437
Number of pages9
JournalApplied Microbiology and Biotechnology
Volume97
Issue number21
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

Fingerprint

Penicillium
Chaetomium
Trametes
Nitrobenzoates
Dithionitrobenzoic Acid
sulfhydryl oxidase
Isoelectric Point
Aspergillus
Substrate Specificity
Sulfhydryl Compounds
Disulfides
Enzymes

Keywords

  • Ellman's reagent
  • enzyme
  • fungi
  • oxidation
  • screening
  • sulfhydryl oxidase
  • thiol

Cite this

Nivala, O. ; Mattinen, Maija ; Faccio, G. ; Buchert, Johanna ; Kruus, Kristiina. / Discovery of novel secreted fungal sulfhydryl oxidases with a plate test screen. In: Applied Microbiology and Biotechnology. 2013 ; Vol. 97, No. 21. pp. 9429-9437.
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Discovery of novel secreted fungal sulfhydryl oxidases with a plate test screen. / Nivala, O.; Mattinen, Maija (Corresponding Author); Faccio, G.; Buchert, Johanna; Kruus, Kristiina.

In: Applied Microbiology and Biotechnology, Vol. 97, No. 21, 2013, p. 9429-9437.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Discovery of novel secreted fungal sulfhydryl oxidases with a plate test screen

AU - Nivala, O.

AU - Mattinen, Maija

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AU - Buchert, Johanna

AU - Kruus, Kristiina

PY - 2013

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AB - Sulfhydryl oxidases (SOX) are FAD-dependent enzymes capable of oxidising free thiol groups and forming disulphide bonds. Although the quantity of scientific papers and suggested applications for SOX is constantly increasing, only a limited number of microbial SOX have been reported and are commercially available. Hence, the aim of this study was to develop a fast and reliable qualitative plate test for screening novel secreted fungal SOX. The screening was based on the Ellman's reagent, i.e. 5,5′-dithiobis[2-nitrobenzoic acid]. Altogether, 32 fungal strains from an in-house culture collection were screened. A total of 13 SOX-producing strains were found positive in the plate test screen. The novel SOX producers were Aspergillus tubingensis, Chaetomium globusum, Melanocarpus albomyces, Penicillium aurantiogriseum, Penicillium funiculosum, Coniophora puteana and Trametes hirsuta. Six of the discovered SOX were partially characterised by determination of isoelectric point, pH optimum and substrate specificity. A. tubingensis was identified as the most efficient novel SOX producer.

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KW - oxidation

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