TY - CHAP
T1 - Disinfectant testing using foodborne bacteria in biofilms
AU - Wirtanen, Gun
AU - Mattila-Sandholm, Tiina
N1 - Project code: ELI22002
PY - 1992
Y1 - 1992
N2 - Biofilm is a phenomenon which develops in several branches of industry, leading however to similar consequences such as increased resistance against antibiotics and disinfectants. In medicine biofilm is called glycocalyx. In processing equipment and circulation systems biofilm protects the microbes against cleaning and sanitation measures. The biofilm layers also trap nutrients, which is an important factor when bacteria grow on surfaces and the nutrient acquisition is limited. These and other results indicate that sanitizer and process disinfectants intended for use in the process industry should be tested on surfaces with test strains important for the industry in question. However, sensitivity tests of antibiotics and disinfectants against microbes are still generally performed using suspension cultures, which normally do not form biofilm. Results achieved in such trials do not correspond with practical situations and they have been called “interesting laboratory exercises”. Biofilm consists of both microbes and surrounding polysaccharides. The formation of biofilm can be induced using low-nutritive substrates, using antibiotics, by stressing the microbes, and above all using surfaces instead of suspensions.
AB - Biofilm is a phenomenon which develops in several branches of industry, leading however to similar consequences such as increased resistance against antibiotics and disinfectants. In medicine biofilm is called glycocalyx. In processing equipment and circulation systems biofilm protects the microbes against cleaning and sanitation measures. The biofilm layers also trap nutrients, which is an important factor when bacteria grow on surfaces and the nutrient acquisition is limited. These and other results indicate that sanitizer and process disinfectants intended for use in the process industry should be tested on surfaces with test strains important for the industry in question. However, sensitivity tests of antibiotics and disinfectants against microbes are still generally performed using suspension cultures, which normally do not form biofilm. Results achieved in such trials do not correspond with practical situations and they have been called “interesting laboratory exercises”. Biofilm consists of both microbes and surrounding polysaccharides. The formation of biofilm can be induced using low-nutritive substrates, using antibiotics, by stressing the microbes, and above all using surfaces instead of suspensions.
U2 - 10.1007/978-94-011-1824-8_53
DO - 10.1007/978-94-011-1824-8_53
M3 - Chapter or book article
SN - 978-94-010-4805-7
T3 - Nato Advanced Study Institutes Series E: Applied Sciences
SP - 595
EP - 600
BT - Biofilms
A2 - Melo, L.F.
A2 - Fletcher, M.
A2 - Capdeville, B.
PB - Kluwer Academic Publishers
CY - Dordrecht
T2 - Biofilms
Y2 - 18 May 1992 through 29 May 1992
ER -