Disposable bioreactors for cultivation of plant cell cultures

Nicolai Lehmann, Ina Dittler, Mari Lämsä, Anneli Ritala, Heiko Rischer, Dieter Eibl, Kirsi-Marja Oksman-Caldentey, Regine Eibl

Research output: Chapter in Book/Report/Conference proceedingChapter or book articleProfessional

9 Citations (Scopus)

Abstract

The trend for using disposable bioreactors in modern biotechnological processes has also been adopted for plant cell cultivations. In fact, plant cell cultures are now being grown in disposable bioreactors with volumes up to 400 L. This trend has been witnessed for both the development and commercial manufacture of therapeutic proteins, secondary metabolite-based pharmaceuticals and cosmetic compounds. Prominent examples of commercial products are Protalix's ELELYSO and Mibelle Biochemistry's Phyto Cell Tech-derived bioactive compounds. This chapter discusses the current state of disposable bioreactor technology for plant cell cultures. After a brief introduction to the general fundamentals of disposable bioreactors (relevant technical terms, advantages and limitations of disposable bioreactors) a current overview of disposable plant cell bioreactors and their instrumentation will be provided. We will describe the working principles and engineering characteristics of disposable bioreactor types that are scalable and successfully being used for the cultivation of plant cell suspension and hairy root cultures. In addition, we will provide selected application examples focusing on the cultivation of geraniol producing tobacco cells. The chapter will end with perspective on future developments of disposable bioreactor technology for plant cell cultures.
Original languageEnglish
Title of host publicationProduction of Biomass and Bioactive Compounds Using Bioreactor Technology
EditorsKee-Yoeup Paek, Niranjana Murthy Hosakatte, Jian-Jiang Zhong
PublisherSpringer
Pages17-46
ISBN (Electronic)978-94-017-9223-3
ISBN (Print)978-94-017-9222-6, 978-94-024-0313-8
DOIs
Publication statusPublished - 2014
MoE publication typeD2 Article in professional manuals or guides or professional information systems or text book material

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Keywords

  • Bubble column
  • Disposable bioreactor technology
  • Hairy root culture
  • Instrumentation
  • Mist bioreactor
  • Orbitally shaken
  • Oscillating
  • Plant cell suspension culture
  • Recombinant protein
  • Secondary metabolite
  • Stirred
  • Tobacco

Cite this

Lehmann, N., Dittler, I., Lämsä, M., Ritala, A., Rischer, H., Eibl, D., Oksman-Caldentey, K-M., & Eibl, R. (2014). Disposable bioreactors for cultivation of plant cell cultures. In K-Y. Paek, N. M. Hosakatte, & J-J. Zhong (Eds.), Production of Biomass and Bioactive Compounds Using Bioreactor Technology (pp. 17-46). Springer. https://doi.org/10.1007/978-94-017-9223-3_2