Disposable bioreactors for cultivation of plant cell cultures

Nicolai Lehmann, Ina Dittler, Mari Lämsä, Anneli Ritala, Heiko Rischer, Dieter Eibl, Kirsi-Marja Oksman-Caldentey, Regine Eibl

Research output: Chapter in Book/Report/Conference proceedingChapter or book articleProfessional

6 Citations (Scopus)

Abstract

The trend for using disposable bioreactors in modern biotechnological processes has also been adopted for plant cell cultivations. In fact, plant cell cultures are now being grown in disposable bioreactors with volumes up to 400 L. This trend has been witnessed for both the development and commercial manufacture of therapeutic proteins, secondary metabolite-based pharmaceuticals and cosmetic compounds. Prominent examples of commercial products are Protalix's ELELYSO and Mibelle Biochemistry's Phyto Cell Tech-derived bioactive compounds. This chapter discusses the current state of disposable bioreactor technology for plant cell cultures. After a brief introduction to the general fundamentals of disposable bioreactors (relevant technical terms, advantages and limitations of disposable bioreactors) a current overview of disposable plant cell bioreactors and their instrumentation will be provided. We will describe the working principles and engineering characteristics of disposable bioreactor types that are scalable and successfully being used for the cultivation of plant cell suspension and hairy root cultures. In addition, we will provide selected application examples focusing on the cultivation of geraniol producing tobacco cells. The chapter will end with perspective on future developments of disposable bioreactor technology for plant cell cultures.
Original languageEnglish
Title of host publicationProduction of Biomass and Bioactive Compounds Using Bioreactor Technology
EditorsKee-Yoeup Paek, Niranjana Murthy Hosakatte, Jian-Jiang Zhong
PublisherSpringer
Pages17-46
ISBN (Electronic)978-94-017-9223-3
ISBN (Print)978-94-017-9222-6, 978-94-024-0313-8
DOIs
Publication statusPublished - 2014
MoE publication typeD2 Article in professional manuals or guides or professional information systems or text book material

Fingerprint

bioreactors
plant cultural practices
cell culture
cells
biopharmaceuticals
geraniol
instrumentation
cosmetics
biochemistry
cell suspension culture
secondary metabolites
manufacturing
engineering
tobacco
drugs

Keywords

  • Bubble column
  • Disposable bioreactor technology
  • Hairy root culture
  • Instrumentation
  • Mist bioreactor
  • Orbitally shaken
  • Oscillating
  • Plant cell suspension culture
  • Recombinant protein
  • Secondary metabolite
  • Stirred
  • Tobacco

Cite this

Lehmann, N., Dittler, I., Lämsä, M., Ritala, A., Rischer, H., Eibl, D., ... Eibl, R. (2014). Disposable bioreactors for cultivation of plant cell cultures. In K-Y. Paek, N. M. Hosakatte, & J-J. Zhong (Eds.), Production of Biomass and Bioactive Compounds Using Bioreactor Technology (pp. 17-46). Springer. https://doi.org/10.1007/978-94-017-9223-3_2
Lehmann, Nicolai ; Dittler, Ina ; Lämsä, Mari ; Ritala, Anneli ; Rischer, Heiko ; Eibl, Dieter ; Oksman-Caldentey, Kirsi-Marja ; Eibl, Regine. / Disposable bioreactors for cultivation of plant cell cultures. Production of Biomass and Bioactive Compounds Using Bioreactor Technology. editor / Kee-Yoeup Paek ; Niranjana Murthy Hosakatte ; Jian-Jiang Zhong. Springer, 2014. pp. 17-46
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abstract = "The trend for using disposable bioreactors in modern biotechnological processes has also been adopted for plant cell cultivations. In fact, plant cell cultures are now being grown in disposable bioreactors with volumes up to 400 L. This trend has been witnessed for both the development and commercial manufacture of therapeutic proteins, secondary metabolite-based pharmaceuticals and cosmetic compounds. Prominent examples of commercial products are Protalix's ELELYSO and Mibelle Biochemistry's Phyto Cell Tech-derived bioactive compounds. This chapter discusses the current state of disposable bioreactor technology for plant cell cultures. After a brief introduction to the general fundamentals of disposable bioreactors (relevant technical terms, advantages and limitations of disposable bioreactors) a current overview of disposable plant cell bioreactors and their instrumentation will be provided. We will describe the working principles and engineering characteristics of disposable bioreactor types that are scalable and successfully being used for the cultivation of plant cell suspension and hairy root cultures. In addition, we will provide selected application examples focusing on the cultivation of geraniol producing tobacco cells. The chapter will end with perspective on future developments of disposable bioreactor technology for plant cell cultures.",
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author = "Nicolai Lehmann and Ina Dittler and Mari L{\"a}ms{\"a} and Anneli Ritala and Heiko Rischer and Dieter Eibl and Kirsi-Marja Oksman-Caldentey and Regine Eibl",
note = "CO:U Z{\"u}rich University of Applied Sciences, Institute of Biotechnology, Biochemical Engineering and Cell Cultivation Technique, W{\"a}denswil, Switzerland CA2: BA3115 CA2: BA311 AU2: L{\"a}ms{\"a}, Mari AU2: Ritala, Anneli AU2: Rischer, Heiko AU2: Oksman-Caldentey, Kirsi-Marja",
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Lehmann, N, Dittler, I, Lämsä, M, Ritala, A, Rischer, H, Eibl, D, Oksman-Caldentey, K-M & Eibl, R 2014, Disposable bioreactors for cultivation of plant cell cultures. in K-Y Paek, NM Hosakatte & J-J Zhong (eds), Production of Biomass and Bioactive Compounds Using Bioreactor Technology. Springer, pp. 17-46. https://doi.org/10.1007/978-94-017-9223-3_2

Disposable bioreactors for cultivation of plant cell cultures. / Lehmann, Nicolai; Dittler, Ina; Lämsä, Mari; Ritala, Anneli; Rischer, Heiko; Eibl, Dieter; Oksman-Caldentey, Kirsi-Marja; Eibl, Regine.

Production of Biomass and Bioactive Compounds Using Bioreactor Technology. ed. / Kee-Yoeup Paek; Niranjana Murthy Hosakatte; Jian-Jiang Zhong. Springer, 2014. p. 17-46.

Research output: Chapter in Book/Report/Conference proceedingChapter or book articleProfessional

TY - CHAP

T1 - Disposable bioreactors for cultivation of plant cell cultures

AU - Lehmann, Nicolai

AU - Dittler, Ina

AU - Lämsä, Mari

AU - Ritala, Anneli

AU - Rischer, Heiko

AU - Eibl, Dieter

AU - Oksman-Caldentey, Kirsi-Marja

AU - Eibl, Regine

N1 - CO:U Zürich University of Applied Sciences, Institute of Biotechnology, Biochemical Engineering and Cell Cultivation Technique, Wädenswil, Switzerland CA2: BA3115 CA2: BA311 AU2: Lämsä, Mari AU2: Ritala, Anneli AU2: Rischer, Heiko AU2: Oksman-Caldentey, Kirsi-Marja

PY - 2014

Y1 - 2014

N2 - The trend for using disposable bioreactors in modern biotechnological processes has also been adopted for plant cell cultivations. In fact, plant cell cultures are now being grown in disposable bioreactors with volumes up to 400 L. This trend has been witnessed for both the development and commercial manufacture of therapeutic proteins, secondary metabolite-based pharmaceuticals and cosmetic compounds. Prominent examples of commercial products are Protalix's ELELYSO and Mibelle Biochemistry's Phyto Cell Tech-derived bioactive compounds. This chapter discusses the current state of disposable bioreactor technology for plant cell cultures. After a brief introduction to the general fundamentals of disposable bioreactors (relevant technical terms, advantages and limitations of disposable bioreactors) a current overview of disposable plant cell bioreactors and their instrumentation will be provided. We will describe the working principles and engineering characteristics of disposable bioreactor types that are scalable and successfully being used for the cultivation of plant cell suspension and hairy root cultures. In addition, we will provide selected application examples focusing on the cultivation of geraniol producing tobacco cells. The chapter will end with perspective on future developments of disposable bioreactor technology for plant cell cultures.

AB - The trend for using disposable bioreactors in modern biotechnological processes has also been adopted for plant cell cultivations. In fact, plant cell cultures are now being grown in disposable bioreactors with volumes up to 400 L. This trend has been witnessed for both the development and commercial manufacture of therapeutic proteins, secondary metabolite-based pharmaceuticals and cosmetic compounds. Prominent examples of commercial products are Protalix's ELELYSO and Mibelle Biochemistry's Phyto Cell Tech-derived bioactive compounds. This chapter discusses the current state of disposable bioreactor technology for plant cell cultures. After a brief introduction to the general fundamentals of disposable bioreactors (relevant technical terms, advantages and limitations of disposable bioreactors) a current overview of disposable plant cell bioreactors and their instrumentation will be provided. We will describe the working principles and engineering characteristics of disposable bioreactor types that are scalable and successfully being used for the cultivation of plant cell suspension and hairy root cultures. In addition, we will provide selected application examples focusing on the cultivation of geraniol producing tobacco cells. The chapter will end with perspective on future developments of disposable bioreactor technology for plant cell cultures.

KW - Bubble column

KW - Disposable bioreactor technology

KW - Hairy root culture

KW - Instrumentation

KW - Mist bioreactor

KW - Orbitally shaken

KW - Oscillating

KW - Plant cell suspension culture

KW - Recombinant protein

KW - Secondary metabolite

KW - Stirred

KW - Tobacco

U2 - 10.1007/978-94-017-9223-3_2

DO - 10.1007/978-94-017-9223-3_2

M3 - Chapter or book article

SN - 978-94-017-9222-6

SN - 978-94-024-0313-8

SP - 17

EP - 46

BT - Production of Biomass and Bioactive Compounds Using Bioreactor Technology

A2 - Paek, Kee-Yoeup

A2 - Hosakatte, Niranjana Murthy

A2 - Zhong, Jian-Jiang

PB - Springer

ER -

Lehmann N, Dittler I, Lämsä M, Ritala A, Rischer H, Eibl D et al. Disposable bioreactors for cultivation of plant cell cultures. In Paek K-Y, Hosakatte NM, Zhong J-J, editors, Production of Biomass and Bioactive Compounds Using Bioreactor Technology. Springer. 2014. p. 17-46 https://doi.org/10.1007/978-94-017-9223-3_2