Disposable roll-to-roll hot embossed electrophoresis chip for detection of antibiotic resistance gene mecA in bacteria

Ralph Liedert; Lotta Amundsen; Ari Hokkanen; Minna Mäki; Anne Aittakorpi; Mikko Pakanen; James R. Schrerer; Richard A. Mathies; Marika Kurkinen; Sanna Uusitalo; Leena Hakalahti; Tarja Nevanen; Harri Siitari; Hans Söderlund

doi:10.1039/C1LC20782B

Disposable roll-to-roll hot embossed electrophoresis chip for detection of antibiotic resistance gene mecA in bacteria

Ralph Liedert (Corresponding Author), Lotta Amundsen, Ari Hokkanen, Minna Mäki, Anne Aittakorpi, Mikko Pakanen, James R. Schrerer, Richard A. Mathies, Marika Kurkinen, Sanna Uusitalo, Leena Hakalahti, Tarja Nevanen, Harri Siitari, Hans Söderlund

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Abstract

We present a high-throughput roll-to-roll (R2R) manufacturing process for foil-based polymethyl methacrylate (PMMA) chips of excellent optical quality. These disposable, R2R hot embossed microfluidic chips are used for the identification of the antibiotic resistance genemecA in Staphylococcus epidermidis. R2R hot embossing is an emerging manufacturing technology for polymer microfluidic devices. It is based on continuous feeding of a thermoplastic foil through a pressurized area between a heated embossing cylinder and a blank counter cylinder. Although mass fabrication of foil-based microfluidic chips and their use for biological applications were foreseen already some years ago, no such studies have been published previously.

Original language	English
Pages (from-to)	333-339
Number of pages	7
Journal	Lab on a Chip
Volume	12
Issue number	2
DOIs	https://doi.org/10.1039/C1LC20782B
Publication status	Published - 2012
MoE publication type	A1 Journal article-refereed

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10.1039/C1LC20782B

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Liedert, R., Amundsen, L., Hokkanen, A., Mäki, M., Aittakorpi, A., Pakanen, M., Schrerer, J. R., Mathies, R. A., Kurkinen, M., Uusitalo, S., Hakalahti, L., Nevanen, T., Siitari, H., & Söderlund, H. (2012). Disposable roll-to-roll hot embossed electrophoresis chip for detection of antibiotic resistance gene mecA in bacteria. Lab on a Chip, 12(2), 333-339. https://doi.org/10.1039/C1LC20782B

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title = "Disposable roll-to-roll hot embossed electrophoresis chip for detection of antibiotic resistance gene mecA in bacteria",

abstract = "We present a high-throughput roll-to-roll (R2R) manufacturing process for foil-based polymethyl methacrylate (PMMA) chips of excellent optical quality. These disposable, R2R hot embossed microfluidic chips are used for the identification of the antibiotic resistance genemecA in Staphylococcus epidermidis. R2R hot embossing is an emerging manufacturing technology for polymer microfluidic devices. It is based on continuous feeding of a thermoplastic foil through a pressurized area between a heated embossing cylinder and a blank counter cylinder. Although mass fabrication of foil-based microfluidic chips and their use for biological applications were foreseen already some years ago, no such studies have been published previously.",

author = "Ralph Liedert and Lotta Amundsen and Ari Hokkanen and Minna M{\"a}ki and Anne Aittakorpi and Mikko Pakanen and Schrerer, {James R.} and Mathies, {Richard A.} and Marika Kurkinen and Sanna Uusitalo and Leena Hakalahti and Tarja Nevanen and Harri Siitari and Hans S{\"o}derlund",

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doi = "10.1039/C1LC20782B",

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Liedert, R, Amundsen, L, Hokkanen, A, Mäki, M, Aittakorpi, A, Pakanen, M, Schrerer, JR, Mathies, RA, Kurkinen, M, Uusitalo, S, Hakalahti, L , Nevanen, T, Siitari, H & Söderlund, H 2012, 'Disposable roll-to-roll hot embossed electrophoresis chip for detection of antibiotic resistance gene mecA in bacteria', Lab on a Chip, vol. 12, no. 2, pp. 333-339. https://doi.org/10.1039/C1LC20782B

TY - JOUR

T1 - Disposable roll-to-roll hot embossed electrophoresis chip for detection of antibiotic resistance gene mecA in bacteria

AU - Liedert, Ralph

AU - Amundsen, Lotta

AU - Hokkanen, Ari

AU - Mäki, Minna

AU - Aittakorpi, Anne

AU - Pakanen, Mikko

AU - Schrerer, James R.

AU - Mathies, Richard A.

AU - Kurkinen, Marika

AU - Uusitalo, Sanna

AU - Hakalahti, Leena

AU - Nevanen, Tarja

AU - Siitari, Harri

AU - Söderlund, Hans

PY - 2012

Y1 - 2012

N2 - We present a high-throughput roll-to-roll (R2R) manufacturing process for foil-based polymethyl methacrylate (PMMA) chips of excellent optical quality. These disposable, R2R hot embossed microfluidic chips are used for the identification of the antibiotic resistance genemecA in Staphylococcus epidermidis. R2R hot embossing is an emerging manufacturing technology for polymer microfluidic devices. It is based on continuous feeding of a thermoplastic foil through a pressurized area between a heated embossing cylinder and a blank counter cylinder. Although mass fabrication of foil-based microfluidic chips and their use for biological applications were foreseen already some years ago, no such studies have been published previously.

AB - We present a high-throughput roll-to-roll (R2R) manufacturing process for foil-based polymethyl methacrylate (PMMA) chips of excellent optical quality. These disposable, R2R hot embossed microfluidic chips are used for the identification of the antibiotic resistance genemecA in Staphylococcus epidermidis. R2R hot embossing is an emerging manufacturing technology for polymer microfluidic devices. It is based on continuous feeding of a thermoplastic foil through a pressurized area between a heated embossing cylinder and a blank counter cylinder. Although mass fabrication of foil-based microfluidic chips and their use for biological applications were foreseen already some years ago, no such studies have been published previously.

U2 - 10.1039/C1LC20782B

DO - 10.1039/C1LC20782B

M3 - Article

SN - 1473-0197

VL - 12

SP - 333

EP - 339

JO - Lab on a Chip

JF - Lab on a Chip

IS - 2

ER -

Disposable roll-to-roll hot embossed electrophoresis chip for detection of antibiotic resistance gene mecA in bacteria

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