Disposable roll-to-roll hot embossed electrophoresis chip for detection of antibiotic resistance gene mecA in bacteria

Ralph Liedert (Corresponding Author), Lotta Amundsen, Ari Hokkanen, Minna Mäki, Anne Aittakorpi, Mikko Pakanen, James R. Schrerer, Richard A. Mathies, Marika Kurkinen, Sanna Uusitalo, Leena Hakalahti, Tarja Nevanen, Harri Siitari, Hans Söderlund

Research output: Contribution to journalArticleScientificpeer-review

43 Citations (Scopus)

Abstract

We present a high-throughput roll-to-roll (R2R) manufacturing process for foil-based polymethyl methacrylate (PMMA) chips of excellent optical quality. These disposable, R2R hot embossed microfluidic chips are used for the identification of the antibiotic resistance genemecA in Staphylococcus epidermidis. R2R hot embossing is an emerging manufacturing technology for polymer microfluidic devices. It is based on continuous feeding of a thermoplastic foil through a pressurized area between a heated embossing cylinder and a blank counter cylinder. Although mass fabrication of foil-based microfluidic chips and their use for biological applications were foreseen already some years ago, no such studies have been published previously.
Original languageEnglish
Pages (from-to)333-339
Number of pages7
JournalLab on a Chip
Volume12
Issue number2
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

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Microfluidics
Antibiotics
Lab-On-A-Chip Devices
Microbial Drug Resistance
Electrophoresis
Metal foil
Bacteria
Genes
Anti-Bacterial Agents
Staphylococcus epidermidis
Polymethyl Methacrylate
Polymers
Technology
Polymethyl methacrylates
Thermoplastics
Throughput
Fabrication

Cite this

Liedert, Ralph ; Amundsen, Lotta ; Hokkanen, Ari ; Mäki, Minna ; Aittakorpi, Anne ; Pakanen, Mikko ; Schrerer, James R. ; Mathies, Richard A. ; Kurkinen, Marika ; Uusitalo, Sanna ; Hakalahti, Leena ; Nevanen, Tarja ; Siitari, Harri ; Söderlund, Hans. / Disposable roll-to-roll hot embossed electrophoresis chip for detection of antibiotic resistance gene mecA in bacteria. In: Lab on a Chip. 2012 ; Vol. 12, No. 2. pp. 333-339.
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abstract = "We present a high-throughput roll-to-roll (R2R) manufacturing process for foil-based polymethyl methacrylate (PMMA) chips of excellent optical quality. These disposable, R2R hot embossed microfluidic chips are used for the identification of the antibiotic resistance genemecA in Staphylococcus epidermidis. R2R hot embossing is an emerging manufacturing technology for polymer microfluidic devices. It is based on continuous feeding of a thermoplastic foil through a pressurized area between a heated embossing cylinder and a blank counter cylinder. Although mass fabrication of foil-based microfluidic chips and their use for biological applications were foreseen already some years ago, no such studies have been published previously.",
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Disposable roll-to-roll hot embossed electrophoresis chip for detection of antibiotic resistance gene mecA in bacteria. / Liedert, Ralph (Corresponding Author); Amundsen, Lotta; Hokkanen, Ari; Mäki, Minna; Aittakorpi, Anne; Pakanen, Mikko; Schrerer, James R.; Mathies, Richard A.; Kurkinen, Marika; Uusitalo, Sanna; Hakalahti, Leena; Nevanen, Tarja; Siitari, Harri; Söderlund, Hans.

In: Lab on a Chip, Vol. 12, No. 2, 2012, p. 333-339.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Liedert, Ralph

AU - Amundsen, Lotta

AU - Hokkanen, Ari

AU - Mäki, Minna

AU - Aittakorpi, Anne

AU - Pakanen, Mikko

AU - Schrerer, James R.

AU - Mathies, Richard A.

AU - Kurkinen, Marika

AU - Uusitalo, Sanna

AU - Hakalahti, Leena

AU - Nevanen, Tarja

AU - Siitari, Harri

AU - Söderlund, Hans

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AB - We present a high-throughput roll-to-roll (R2R) manufacturing process for foil-based polymethyl methacrylate (PMMA) chips of excellent optical quality. These disposable, R2R hot embossed microfluidic chips are used for the identification of the antibiotic resistance genemecA in Staphylococcus epidermidis. R2R hot embossing is an emerging manufacturing technology for polymer microfluidic devices. It is based on continuous feeding of a thermoplastic foil through a pressurized area between a heated embossing cylinder and a blank counter cylinder. Although mass fabrication of foil-based microfluidic chips and their use for biological applications were foreseen already some years ago, no such studies have been published previously.

U2 - 10.1039/C1LC20782B

DO - 10.1039/C1LC20782B

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