Abstract
Desulfovibrio species belong to sulfate-reducing bacteria (SRB), which
constitute a diverse group of prokaryotes that contribute to a variety of
essential functions in many anaerobic environments. However, some
Desulfovibrio spp. cause infrequently a variety of human infections - for
example they have been postulated to have a role in ulcerative colitis.
Detailed analysis of occurrence and abundance of SRB within complex microbial
communities has formerly been restricted to information obtained by
conventional culture techniques and has been biased by the inability to
cultivate most of the organisms. However, the application of molecular methods
enables a direct visualization of bacterial diversity, monitoring and
enumeration of the dominant population, and the opportunity for subsequent
identification of community members by sequence analysis. The aim of the
present study was to investigate the diversity of Desulfovibrionaceae
populations in feces of IBS (irritable bowel syndrome) and control subjects.
Moreover, the changes in stability of fecal Desulfovibrionaceae populations of
5 control subjects were followed over a period of 2 years including a period
of probiotic ingestion. Furthermore, the similarity and stability of the fecal
and salivary Desulfovibrionaceae populations in these subjects was monitored
and compared. Fecal samples of 21 IBS patients and 18 control subjects in
addition to fecal and salivary samples (3 samples from each subject before and
during the feeding trial) of 5 control subjects were analyzed with PCR-DGGE
(denaturing gradient gel electrophoresis). According to the DGGE results there
were more bands and more variability in the DGGE profiles of IBS patients
than in the DGGE profiles of control subjects. Furthermore, differences in
intensities as detected by gel electrophoresis after group-specific PCR were
considerably large. Therefore, FISH (fluorescent in situ hybridization) was
used to quantify the number of sulfate-reducing bacteria and the number of
Desulfovibrio spp. in fecal and salivary samples.
| Original language | English |
|---|---|
| Title of host publication | The Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop |
| Subtitle of host publication | Abstracts and posters |
| Place of Publication | Espoo |
| Publisher | VTT Technical Research Centre of Finland |
| Pages | 80-80 |
| ISBN (Electronic) | 951-38-6290-9 |
| ISBN (Print) | 951-38-6289-5 |
| Publication status | Published - 2004 |
| Event | The Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop - Sitges, Spain Duration: 15 Mar 2004 → 17 Mar 2004 |
Publication series
| Series | VTT Symposium |
|---|---|
| Number | 232 |
| ISSN | 0357-9387 |
Workshop
| Workshop | The Food, GI-tract Functionality and Human Health Cluster |
|---|---|
| Country | Spain |
| City | Sitges |
| Period | 15/03/04 → 17/03/04 |
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Cite this
Maukonen, J., Mättö, J., & Saarela, M. (2004). Diversity and abundance of Desulfovibrionaceae related populations in feces and saliva as detected by group-specific PCR-DGGE and FISH. In
The Food, GI-tract Functionality and Human Health Cluster: 3rd
PROEUHEALTH Workshop
: Abstracts and posters (pp. 80-80). VTT Technical Research Centre of Finland. VTT Symposium, No. 232