Diversity and abundance of Desulfovibrionaceae related populations in feces and saliva as detected by group-specific PCR-DGGE and FISH

Johanna Maukonen, Jaana Mättö, Maria Saarela

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

Desulfovibrio species belong to sulfate-reducing bacteria (SRB), which constitute a diverse group of prokaryotes that contribute to a variety of essential functions in many anaerobic environments. However, some Desulfovibrio spp. cause infrequently a variety of human infections - for example they have been postulated to have a role in ulcerative colitis. Detailed analysis of occurrence and abundance of SRB within complex microbial communities has formerly been restricted to information obtained by conventional culture techniques and has been biased by the inability to cultivate most of the organisms. However, the application of molecular methods enables a direct visualization of bacterial diversity, monitoring and enumeration of the dominant population, and the opportunity for subsequent identification of community members by sequence analysis. The aim of the present study was to investigate the diversity of Desulfovibrionaceae populations in feces of IBS (irritable bowel syndrome) and control subjects. Moreover, the changes in stability of fecal Desulfovibrionaceae populations of 5 control subjects were followed over a period of 2 years including a period of probiotic ingestion. Furthermore, the similarity and stability of the fecal and salivary Desulfovibrionaceae populations in these subjects was monitored and compared. Fecal samples of 21 IBS patients and 18 control subjects in addition to fecal and salivary samples (3 samples from each subject before and during the feeding trial) of 5 control subjects were analyzed with PCR-DGGE (denaturing gradient gel electrophoresis). According to the DGGE results there were more bands and more variability in the DGGE profiles of IBS patients than in the DGGE profiles of control subjects. Furthermore, differences in intensities as detected by gel electrophoresis after group-specific PCR were considerably large. Therefore, FISH (fluorescent in situ hybridization) was used to quantify the number of sulfate-reducing bacteria and the number of Desulfovibrio spp. in fecal and salivary samples.
Original languageEnglish
Title of host publication The Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop
Subtitle of host publicationAbstracts and posters
Place of PublicationEspoo
PublisherVTT Technical Research Centre of Finland
Pages80-80
ISBN (Electronic)951-38-6290-9
ISBN (Print)951-38-6289-5
Publication statusPublished - 2004
EventThe Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop - Sitges, Spain
Duration: 15 Mar 200417 Mar 2004

Publication series

NameVTT Symposium
PublisherVTT
Number232
ISSN (Print)0357-9387
ISSN (Electronic)1455-0873

Workshop

WorkshopThe Food, GI-tract Functionality and Human Health Cluster
CountrySpain
CitySitges
Period15/03/0417/03/04

Fingerprint

Desulfovibrio
Irritable Bowel Syndrome
Fluorescence In Situ Hybridization
Saliva
Feces
Sulfates
Bacteria
Polymerase Chain Reaction
Population
Culture Techniques
Denaturing Gradient Gel Electrophoresis
Population Control
Probiotics
Ulcerative Colitis
Sequence Analysis
Electrophoresis
Eating
Gels
Infection

Cite this

Maukonen, J., Mättö, J., & Saarela, M. (2004). Diversity and abundance of Desulfovibrionaceae related populations in feces and saliva as detected by group-specific PCR-DGGE and FISH. In The Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop : Abstracts and posters (pp. 80-80). Espoo: VTT Technical Research Centre of Finland. VTT Symposium, No. 232
Maukonen, Johanna ; Mättö, Jaana ; Saarela, Maria. / Diversity and abundance of Desulfovibrionaceae related populations in feces and saliva as detected by group-specific PCR-DGGE and FISH. The Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop : Abstracts and posters. Espoo : VTT Technical Research Centre of Finland, 2004. pp. 80-80 (VTT Symposium; No. 232).
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Maukonen, J, Mättö, J & Saarela, M 2004, Diversity and abundance of Desulfovibrionaceae related populations in feces and saliva as detected by group-specific PCR-DGGE and FISH. in The Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop : Abstracts and posters. VTT Technical Research Centre of Finland, Espoo, VTT Symposium, no. 232, pp. 80-80, The Food, GI-tract Functionality and Human Health Cluster, Sitges, Spain, 15/03/04.

Diversity and abundance of Desulfovibrionaceae related populations in feces and saliva as detected by group-specific PCR-DGGE and FISH. / Maukonen, Johanna; Mättö, Jaana; Saarela, Maria.

The Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop : Abstracts and posters. Espoo : VTT Technical Research Centre of Finland, 2004. p. 80-80 (VTT Symposium; No. 232).

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

TY - CHAP

T1 - Diversity and abundance of Desulfovibrionaceae related populations in feces and saliva as detected by group-specific PCR-DGGE and FISH

AU - Maukonen, Johanna

AU - Mättö, Jaana

AU - Saarela, Maria

PY - 2004

Y1 - 2004

N2 - Desulfovibrio species belong to sulfate-reducing bacteria (SRB), which constitute a diverse group of prokaryotes that contribute to a variety of essential functions in many anaerobic environments. However, some Desulfovibrio spp. cause infrequently a variety of human infections - for example they have been postulated to have a role in ulcerative colitis. Detailed analysis of occurrence and abundance of SRB within complex microbial communities has formerly been restricted to information obtained by conventional culture techniques and has been biased by the inability to cultivate most of the organisms. However, the application of molecular methods enables a direct visualization of bacterial diversity, monitoring and enumeration of the dominant population, and the opportunity for subsequent identification of community members by sequence analysis. The aim of the present study was to investigate the diversity of Desulfovibrionaceae populations in feces of IBS (irritable bowel syndrome) and control subjects. Moreover, the changes in stability of fecal Desulfovibrionaceae populations of 5 control subjects were followed over a period of 2 years including a period of probiotic ingestion. Furthermore, the similarity and stability of the fecal and salivary Desulfovibrionaceae populations in these subjects was monitored and compared. Fecal samples of 21 IBS patients and 18 control subjects in addition to fecal and salivary samples (3 samples from each subject before and during the feeding trial) of 5 control subjects were analyzed with PCR-DGGE (denaturing gradient gel electrophoresis). According to the DGGE results there were more bands and more variability in the DGGE profiles of IBS patients than in the DGGE profiles of control subjects. Furthermore, differences in intensities as detected by gel electrophoresis after group-specific PCR were considerably large. Therefore, FISH (fluorescent in situ hybridization) was used to quantify the number of sulfate-reducing bacteria and the number of Desulfovibrio spp. in fecal and salivary samples.

AB - Desulfovibrio species belong to sulfate-reducing bacteria (SRB), which constitute a diverse group of prokaryotes that contribute to a variety of essential functions in many anaerobic environments. However, some Desulfovibrio spp. cause infrequently a variety of human infections - for example they have been postulated to have a role in ulcerative colitis. Detailed analysis of occurrence and abundance of SRB within complex microbial communities has formerly been restricted to information obtained by conventional culture techniques and has been biased by the inability to cultivate most of the organisms. However, the application of molecular methods enables a direct visualization of bacterial diversity, monitoring and enumeration of the dominant population, and the opportunity for subsequent identification of community members by sequence analysis. The aim of the present study was to investigate the diversity of Desulfovibrionaceae populations in feces of IBS (irritable bowel syndrome) and control subjects. Moreover, the changes in stability of fecal Desulfovibrionaceae populations of 5 control subjects were followed over a period of 2 years including a period of probiotic ingestion. Furthermore, the similarity and stability of the fecal and salivary Desulfovibrionaceae populations in these subjects was monitored and compared. Fecal samples of 21 IBS patients and 18 control subjects in addition to fecal and salivary samples (3 samples from each subject before and during the feeding trial) of 5 control subjects were analyzed with PCR-DGGE (denaturing gradient gel electrophoresis). According to the DGGE results there were more bands and more variability in the DGGE profiles of IBS patients than in the DGGE profiles of control subjects. Furthermore, differences in intensities as detected by gel electrophoresis after group-specific PCR were considerably large. Therefore, FISH (fluorescent in situ hybridization) was used to quantify the number of sulfate-reducing bacteria and the number of Desulfovibrio spp. in fecal and salivary samples.

M3 - Conference abstract in proceedings

SN - 951-38-6289-5

T3 - VTT Symposium

SP - 80

EP - 80

BT - The Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop

PB - VTT Technical Research Centre of Finland

CY - Espoo

ER -

Maukonen J, Mättö J, Saarela M. Diversity and abundance of Desulfovibrionaceae related populations in feces and saliva as detected by group-specific PCR-DGGE and FISH. In The Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop : Abstracts and posters. Espoo: VTT Technical Research Centre of Finland. 2004. p. 80-80. (VTT Symposium; No. 232).