Diversity and abundance of Desulfovibrionaceae related populations in feces and saliva as detected by group-specific PCR-DGGE and FISH

Johanna Maukonen, Jaana Mättö, Maria Saarela

    Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

    Abstract

    Desulfovibrio species belong to sulfate-reducing bacteria (SRB), which constitute a diverse group of prokaryotes that contribute to a variety of essential functions in many anaerobic environments. However, some Desulfovibrio spp. cause infrequently a variety of human infections - for example they have been postulated to have a role in ulcerative colitis. Detailed analysis of occurrence and abundance of SRB within complex microbial communities has formerly been restricted to information obtained by conventional culture techniques and has been biased by the inability to cultivate most of the organisms. However, the application of molecular methods enables a direct visualization of bacterial diversity, monitoring and enumeration of the dominant population, and the opportunity for subsequent identification of community members by sequence analysis. The aim of the present study was to investigate the diversity of Desulfovibrionaceae populations in feces of IBS (irritable bowel syndrome) and control subjects. Moreover, the changes in stability of fecal Desulfovibrionaceae populations of 5 control subjects were followed over a period of 2 years including a period of probiotic ingestion. Furthermore, the similarity and stability of the fecal and salivary Desulfovibrionaceae populations in these subjects was monitored and compared. Fecal samples of 21 IBS patients and 18 control subjects in addition to fecal and salivary samples (3 samples from each subject before and during the feeding trial) of 5 control subjects were analyzed with PCR-DGGE (denaturing gradient gel electrophoresis). According to the DGGE results there were more bands and more variability in the DGGE profiles of IBS patients than in the DGGE profiles of control subjects. Furthermore, differences in intensities as detected by gel electrophoresis after group-specific PCR were considerably large. Therefore, FISH (fluorescent in situ hybridization) was used to quantify the number of sulfate-reducing bacteria and the number of Desulfovibrio spp. in fecal and salivary samples.
    Original languageEnglish
    Title of host publication The Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop
    Subtitle of host publicationAbstracts and posters
    Place of PublicationEspoo
    PublisherVTT Technical Research Centre of Finland
    Pages80-80
    ISBN (Electronic)951-38-6290-9
    ISBN (Print)951-38-6289-5
    Publication statusPublished - 2004
    EventThe Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop - Sitges, Spain
    Duration: 15 Mar 200417 Mar 2004

    Publication series

    SeriesVTT Symposium
    Number232
    ISSN0357-9387

    Workshop

    WorkshopThe Food, GI-tract Functionality and Human Health Cluster
    Country/TerritorySpain
    CitySitges
    Period15/03/0417/03/04

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