Diversity and abundance of Desulfovibrionaceae related populations in feces and saliva as detected by group-specific PCR-DGGE and FISH

Johanna Maukonen; Jaana Mättö; Maria Saarela

Diversity and abundance of Desulfovibrionaceae related populations in feces and saliva as detected by group-specific PCR-DGGE and FISH

Johanna Maukonen, Jaana Mättö, Maria Saarela

BA3407 Process microbiology and food safety

Research output: Chapter in Book/Report/Conference proceeding › Conference abstract in proceedings › Scientific

Abstract

Desulfovibrio species belong to sulfate-reducing bacteria (SRB), which constitute a diverse group of prokaryotes that contribute to a variety of essential functions in many anaerobic environments. However, some Desulfovibrio spp. cause infrequently a variety of human infections - for example they have been postulated to have a role in ulcerative colitis. Detailed analysis of occurrence and abundance of SRB within complex microbial communities has formerly been restricted to information obtained by conventional culture techniques and has been biased by the inability to cultivate most of the organisms. However, the application of molecular methods enables a direct visualization of bacterial diversity, monitoring and enumeration of the dominant population, and the opportunity for subsequent identification of community members by sequence analysis. The aim of the present study was to investigate the diversity of Desulfovibrionaceae populations in feces of IBS (irritable bowel syndrome) and control subjects. Moreover, the changes in stability of fecal Desulfovibrionaceae populations of 5 control subjects were followed over a period of 2 years including a period of probiotic ingestion. Furthermore, the similarity and stability of the fecal and salivary Desulfovibrionaceae populations in these subjects was monitored and compared. Fecal samples of 21 IBS patients and 18 control subjects in addition to fecal and salivary samples (3 samples from each subject before and during the feeding trial) of 5 control subjects were analyzed with PCR-DGGE (denaturing gradient gel electrophoresis). According to the DGGE results there were more bands and more variability in the DGGE profiles of IBS patients than in the DGGE profiles of control subjects. Furthermore, differences in intensities as detected by gel electrophoresis after group-specific PCR were considerably large. Therefore, FISH (fluorescent in situ hybridization) was used to quantify the number of sulfate-reducing bacteria and the number of Desulfovibrio spp. in fecal and salivary samples.

Original language	English
Title of host publication	The Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop
Subtitle of host publication	Abstracts and posters
Place of Publication	Espoo
Publisher	VTT Technical Research Centre of Finland
Pages	80-80
ISBN (Electronic)	951-38-6290-9
ISBN (Print)	951-38-6289-5
Publication status	Published - 2004
Event	The Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop - Sitges, Spain Duration: 15 Mar 2004 → 17 Mar 2004

Publication series

Series	VTT Symposium
Number	232
ISSN	0357-9387

Workshop

Workshop	The Food, GI-tract Functionality and Human Health Cluster
Country	Spain
City	Sitges
Period	15/03/04 → 17/03/04

Access to Document

http://www.vtt.fi/inf/pdf/symposiums/2004/S232.pdf

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Maukonen, J., Mättö, J., & Saarela, M. (2004). Diversity and abundance of Desulfovibrionaceae related populations in feces and saliva as detected by group-specific PCR-DGGE and FISH. In The Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop : Abstracts and posters (pp. 80-80). VTT Technical Research Centre of Finland. VTT Symposium, No. 232 http://www.vtt.fi/inf/pdf/symposiums/2004/S232.pdf

Maukonen, Johanna ; Mättö, Jaana ; Saarela, Maria. / Diversity and abundance of Desulfovibrionaceae related populations in feces and saliva as detected by group-specific PCR-DGGE and FISH. The Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop : Abstracts and posters. Espoo : VTT Technical Research Centre of Finland, 2004. pp. 80-80 (VTT Symposium; No. 232).

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abstract = "Desulfovibrio species belong to sulfate-reducing bacteria (SRB), which constitute a diverse group of prokaryotes that contribute to a variety of essential functions in many anaerobic environments. However, some Desulfovibrio spp. cause infrequently a variety of human infections - for example they have been postulated to have a role in ulcerative colitis. Detailed analysis of occurrence and abundance of SRB within complex microbial communities has formerly been restricted to information obtained by conventional culture techniques and has been biased by the inability to cultivate most of the organisms. However, the application of molecular methods enables a direct visualization of bacterial diversity, monitoring and enumeration of the dominant population, and the opportunity for subsequent identification of community members by sequence analysis. The aim of the present study was to investigate the diversity of Desulfovibrionaceae populations in feces of IBS (irritable bowel syndrome) and control subjects. Moreover, the changes in stability of fecal Desulfovibrionaceae populations of 5 control subjects were followed over a period of 2 years including a period of probiotic ingestion. Furthermore, the similarity and stability of the fecal and salivary Desulfovibrionaceae populations in these subjects was monitored and compared. Fecal samples of 21 IBS patients and 18 control subjects in addition to fecal and salivary samples (3 samples from each subject before and during the feeding trial) of 5 control subjects were analyzed with PCR-DGGE (denaturing gradient gel electrophoresis). According to the DGGE results there were more bands and more variability in the DGGE profiles of IBS patients than in the DGGE profiles of control subjects. Furthermore, differences in intensities as detected by gel electrophoresis after group-specific PCR were considerably large. Therefore, FISH (fluorescent in situ hybridization) was used to quantify the number of sulfate-reducing bacteria and the number of Desulfovibrio spp. in fecal and salivary samples.",

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Maukonen, J, Mättö, J & Saarela, M 2004, Diversity and abundance of Desulfovibrionaceae related populations in feces and saliva as detected by group-specific PCR-DGGE and FISH. in The Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop : Abstracts and posters. VTT Technical Research Centre of Finland, Espoo, VTT Symposium, no. 232, pp. 80-80, The Food, GI-tract Functionality and Human Health Cluster, Sitges, Spain, 15/03/04. <http://www.vtt.fi/inf/pdf/symposiums/2004/S232.pdf>

Diversity and abundance of Desulfovibrionaceae related populations in feces and saliva as detected by group-specific PCR-DGGE and FISH. / Maukonen, Johanna; Mättö, Jaana; Saarela, Maria.

The Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop : Abstracts and posters. Espoo : VTT Technical Research Centre of Finland, 2004. p. 80-80 (VTT Symposium; No. 232).

Research output: Chapter in Book/Report/Conference proceeding › Conference abstract in proceedings › Scientific

TY - CHAP

T1 - Diversity and abundance of Desulfovibrionaceae related populations in feces and saliva as detected by group-specific PCR-DGGE and FISH

AU - Maukonen, Johanna

AU - Mättö, Jaana

AU - Saarela, Maria

PY - 2004

Y1 - 2004

N2 - Desulfovibrio species belong to sulfate-reducing bacteria (SRB), which constitute a diverse group of prokaryotes that contribute to a variety of essential functions in many anaerobic environments. However, some Desulfovibrio spp. cause infrequently a variety of human infections - for example they have been postulated to have a role in ulcerative colitis. Detailed analysis of occurrence and abundance of SRB within complex microbial communities has formerly been restricted to information obtained by conventional culture techniques and has been biased by the inability to cultivate most of the organisms. However, the application of molecular methods enables a direct visualization of bacterial diversity, monitoring and enumeration of the dominant population, and the opportunity for subsequent identification of community members by sequence analysis. The aim of the present study was to investigate the diversity of Desulfovibrionaceae populations in feces of IBS (irritable bowel syndrome) and control subjects. Moreover, the changes in stability of fecal Desulfovibrionaceae populations of 5 control subjects were followed over a period of 2 years including a period of probiotic ingestion. Furthermore, the similarity and stability of the fecal and salivary Desulfovibrionaceae populations in these subjects was monitored and compared. Fecal samples of 21 IBS patients and 18 control subjects in addition to fecal and salivary samples (3 samples from each subject before and during the feeding trial) of 5 control subjects were analyzed with PCR-DGGE (denaturing gradient gel electrophoresis). According to the DGGE results there were more bands and more variability in the DGGE profiles of IBS patients than in the DGGE profiles of control subjects. Furthermore, differences in intensities as detected by gel electrophoresis after group-specific PCR were considerably large. Therefore, FISH (fluorescent in situ hybridization) was used to quantify the number of sulfate-reducing bacteria and the number of Desulfovibrio spp. in fecal and salivary samples.

AB - Desulfovibrio species belong to sulfate-reducing bacteria (SRB), which constitute a diverse group of prokaryotes that contribute to a variety of essential functions in many anaerobic environments. However, some Desulfovibrio spp. cause infrequently a variety of human infections - for example they have been postulated to have a role in ulcerative colitis. Detailed analysis of occurrence and abundance of SRB within complex microbial communities has formerly been restricted to information obtained by conventional culture techniques and has been biased by the inability to cultivate most of the organisms. However, the application of molecular methods enables a direct visualization of bacterial diversity, monitoring and enumeration of the dominant population, and the opportunity for subsequent identification of community members by sequence analysis. The aim of the present study was to investigate the diversity of Desulfovibrionaceae populations in feces of IBS (irritable bowel syndrome) and control subjects. Moreover, the changes in stability of fecal Desulfovibrionaceae populations of 5 control subjects were followed over a period of 2 years including a period of probiotic ingestion. Furthermore, the similarity and stability of the fecal and salivary Desulfovibrionaceae populations in these subjects was monitored and compared. Fecal samples of 21 IBS patients and 18 control subjects in addition to fecal and salivary samples (3 samples from each subject before and during the feeding trial) of 5 control subjects were analyzed with PCR-DGGE (denaturing gradient gel electrophoresis). According to the DGGE results there were more bands and more variability in the DGGE profiles of IBS patients than in the DGGE profiles of control subjects. Furthermore, differences in intensities as detected by gel electrophoresis after group-specific PCR were considerably large. Therefore, FISH (fluorescent in situ hybridization) was used to quantify the number of sulfate-reducing bacteria and the number of Desulfovibrio spp. in fecal and salivary samples.

M3 - Conference abstract in proceedings

SN - 951-38-6289-5

T3 - VTT Symposium

SP - 80

EP - 80

BT - The Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop

PB - VTT Technical Research Centre of Finland

CY - Espoo

T2 - The Food, GI-tract Functionality and Human Health Cluster

Y2 - 15 March 2004 through 17 March 2004

ER -

Maukonen J, Mättö J, Saarela M. Diversity and abundance of Desulfovibrionaceae related populations in feces and saliva as detected by group-specific PCR-DGGE and FISH. In The Food, GI-tract Functionality and Human Health Cluster: 3rd PROEUHEALTH Workshop : Abstracts and posters. Espoo: VTT Technical Research Centre of Finland. 2004. p. 80-80. (VTT Symposium; No. 232).