DNA polymerase e catalytic domains are dispensable for DNA replication, DNA repair, and cell viability

Tapio Kesti, Karin Flinck, Sirkka Keränen, Juhani Syväoja, Curt Wittenberg (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

204 Citations (Scopus)

Abstract

DNA polymerase ε (Pol ε) is believed to play an essential catalytic role during eukaryotic DNA replication and is thought to participate in recombination and DNA repair. That Pol ε is essential for progression through S phase and for viability in budding and fission yeasts is a central element of support for that view. We show that the amino-terminal portion of budding yeast Pol ε (Pol2) containing all known DNA polymerase and exonuclease motifs is dispensable for DNA replication, DNA repair, and viability. However, the carboxy-terminal portion of Pol2 is both necessary and sufficient for viability. Finally, the viability of cells lacking Pol2 catalytic function does not require intact DNA replication or damage checkpoints.
Original languageEnglish
Pages (from-to)679-685
JournalMolecular Cell
Volume3
Issue number5
DOIs
Publication statusPublished - 1999
MoE publication typeA1 Journal article-refereed

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