Abstract
DNA polymerase ε (Pol ε) is believed to play an essential catalytic role during eukaryotic DNA replication and is thought to participate in recombination and DNA repair. That Pol ε is essential for progression through S phase and for viability in budding and fission yeasts is a central element of support for that view. We show that the amino-terminal portion of budding yeast Pol ε (Pol2) containing all known DNA polymerase and exonuclease motifs is dispensable for DNA replication, DNA repair, and viability. However, the carboxy-terminal portion of Pol2 is both necessary and sufficient for viability. Finally, the viability of cells lacking Pol2 catalytic function does not require intact DNA replication or damage checkpoints.
| Original language | English |
|---|---|
| Pages (from-to) | 679-685 |
| Journal | Molecular Cell |
| Volume | 3 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1999 |
| MoE publication type | A1 Journal article-refereed |
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Kesti, T., Flinck, K., Keränen, S., Syväoja, J., & Wittenberg, C. (1999). DNA polymerase e catalytic domains are dispensable for DNA replication, DNA repair, and cell viability. Molecular Cell, 3(5), 679-685. https://doi.org/10.1016/S1097-2765(00)80361-5