Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 679-685 |
| Number of pages | 7 |
| Journal | Molecular Cell |
| Volume | 3 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1999 |
| MoE publication type | A1 Journal article-refereed |
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DNA polymerase e catalytic domains are dispensable for DNA replication, DNA repair, and cell viability. / Kesti, Tapio; Flinck, Karin; Keränen, Sirkka; Syväoja, Juhani; Wittenberg, Curt (Corresponding Author).
In: Molecular Cell, Vol. 3, No. 5, 1999, p. 679-685.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - DNA polymerase e catalytic domains are dispensable for DNA replication, DNA repair, and cell viability
AU - Kesti, Tapio
AU - Flinck, Karin
AU - Keränen, Sirkka
AU - Syväoja, Juhani
AU - Wittenberg, Curt
PY - 1999
Y1 - 1999
N2 - DNA polymerase ε (Pol ε) is believed to play an essential catalytic role during eukaryotic DNA replication and is thought to participate in recombination and DNA repair. That Pol ε is essential for progression through S phase and for viability in budding and fission yeasts is a central element of support for that view. We show that the amino-terminal portion of budding yeast Pol ε (Pol2) containing all known DNA polymerase and exonuclease motifs is dispensable for DNA replication, DNA repair, and viability. However, the carboxy-terminal portion of Pol2 is both necessary and sufficient for viability. Finally, the viability of cells lacking Pol2 catalytic function does not require intact DNA replication or damage checkpoints.
AB - DNA polymerase ε (Pol ε) is believed to play an essential catalytic role during eukaryotic DNA replication and is thought to participate in recombination and DNA repair. That Pol ε is essential for progression through S phase and for viability in budding and fission yeasts is a central element of support for that view. We show that the amino-terminal portion of budding yeast Pol ε (Pol2) containing all known DNA polymerase and exonuclease motifs is dispensable for DNA replication, DNA repair, and viability. However, the carboxy-terminal portion of Pol2 is both necessary and sufficient for viability. Finally, the viability of cells lacking Pol2 catalytic function does not require intact DNA replication or damage checkpoints.
U2 - 10.1016/S1097-2765(00)80361-5
DO - 10.1016/S1097-2765(00)80361-5
M3 - Article
VL - 3
SP - 679
EP - 685
JO - Molecular Cell
JF - Molecular Cell
SN - 1097-2765
IS - 5
ER -