Downstream Processing of Extracellular Enzymes of Aspergillus Niger

Michael Bailey, Heikki Ojamo

Research output: Chapter in Book/Report/Conference proceedingChapter or book articleScientificpeer-review

Abstract

Different strategies were compared for the concentration of polygalacturonase, ß-glucosidase and ß-galactosidase produced by two strains of Aspergillus niger. Ultrafiltration was more successful than vacuum evaporation for all three enzymes but produced concentrates with high solids contents. The use of mineral adsorbents enabled almost complete separation and concentration of polygalacturonase and ß-glucosidase activities from one culture filtrate. Concentration of all three enzymes using adsorbents led to preparations with higher specific activities and lower solids contents than the corresponding ultrafiltration concentrates.
Original languageEnglish
Title of host publicationSeparations for Biotechnology
EditorsD.L. Pyle
Place of PublicationLondon
PublisherElsevier
Pages529-538
Volume2
ISBN (Electronic)978-94-009-0783-6
ISBN (Print)978-94-010-6839-0
DOIs
Publication statusPublished - 1990
MoE publication typeA3 Part of a book or another research book

Fingerprint

glucosidases
adsorbents
ultrafiltration
polygalacturonase
Aspergillus niger
concentrates
galactosidases
culture filtrates
enzymes
evaporation
minerals

Cite this

Bailey, M., & Ojamo, H. (1990). Downstream Processing of Extracellular Enzymes of Aspergillus Niger. In D. L. Pyle (Ed.), Separations for Biotechnology (Vol. 2, pp. 529-538). London: Elsevier. https://doi.org/10.1007/978-94-009-0783-6_56
Bailey, Michael ; Ojamo, Heikki. / Downstream Processing of Extracellular Enzymes of Aspergillus Niger. Separations for Biotechnology. editor / D.L. Pyle. Vol. 2 London : Elsevier, 1990. pp. 529-538
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Bailey, M & Ojamo, H 1990, Downstream Processing of Extracellular Enzymes of Aspergillus Niger. in DL Pyle (ed.), Separations for Biotechnology. vol. 2, Elsevier, London, pp. 529-538. https://doi.org/10.1007/978-94-009-0783-6_56

Downstream Processing of Extracellular Enzymes of Aspergillus Niger. / Bailey, Michael; Ojamo, Heikki.

Separations for Biotechnology. ed. / D.L. Pyle. Vol. 2 London : Elsevier, 1990. p. 529-538.

Research output: Chapter in Book/Report/Conference proceedingChapter or book articleScientificpeer-review

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Bailey M, Ojamo H. Downstream Processing of Extracellular Enzymes of Aspergillus Niger. In Pyle DL, editor, Separations for Biotechnology. Vol. 2. London: Elsevier. 1990. p. 529-538 https://doi.org/10.1007/978-94-009-0783-6_56