Different strategies were compared for the concentration of polygalacturonase, ß-glucosidase and ß-galactosidase produced by two strains of Aspergillus niger. Ultrafiltration was more successful than vacuum evaporation for all three enzymes but produced concentrates with high solids contents. The use of mineral adsorbents enabled almost complete separation and concentration of polygalacturonase and ß-glucosidase activities from one culture filtrate. Concentration of all three enzymes using adsorbents led to preparations with higher specific activities and lower solids contents than the corresponding ultrafiltration concentrates.
|Title of host publication||Separations for Biotechnology|
|Place of Publication||London|
|Publication status||Published - 1990|
|MoE publication type||A3 Part of a book or another research book|
Bailey, M., & Ojamo, H. (1990). Downstream Processing of Extracellular Enzymes of Aspergillus Niger. In D. L. Pyle (Ed.), Separations for Biotechnology (Vol. 2, pp. 529-538). Elsevier. https://doi.org/10.1007/978-94-009-0783-6_56