Downstream Processing of Extracellular Enzymes of Aspergillus Niger

Michael Bailey; Heikki Ojamo

doi:10.1007/978-94-009-0783-6_56

Downstream Processing of Extracellular Enzymes of Aspergillus Niger

Michael Bailey, Heikki Ojamo

VTT Technical Research Centre of Finland

Research output: Chapter in Book/Report/Conference proceeding › Chapter or book article › Scientific › peer-review

Abstract

Different strategies were compared for the concentration of polygalacturonase, ß-glucosidase and ß-galactosidase produced by two strains of Aspergillus niger. Ultrafiltration was more successful than vacuum evaporation for all three enzymes but produced concentrates with high solids contents. The use of mineral adsorbents enabled almost complete separation and concentration of polygalacturonase and ß-glucosidase activities from one culture filtrate. Concentration of all three enzymes using adsorbents led to preparations with higher specific activities and lower solids contents than the corresponding ultrafiltration concentrates.

Original language	English
Title of host publication	Separations for Biotechnology
Editors	D.L. Pyle
Place of Publication	London
Publisher	Elsevier
Pages	529-538
Volume	2
ISBN (Electronic)	978-94-009-0783-6
ISBN (Print)	978-94-010-6839-0
DOIs	https://doi.org/10.1007/978-94-009-0783-6_56
Publication status	Published - 1990
MoE publication type	A3 Part of a book or another research book

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abstract = "Different strategies were compared for the concentration of polygalacturonase, {\ss}-glucosidase and {\ss}-galactosidase produced by two strains of Aspergillus niger. Ultrafiltration was more successful than vacuum evaporation for all three enzymes but produced concentrates with high solids contents. The use of mineral adsorbents enabled almost complete separation and concentration of polygalacturonase and {\ss}-glucosidase activities from one culture filtrate. Concentration of all three enzymes using adsorbents led to preparations with higher specific activities and lower solids contents than the corresponding ultrafiltration concentrates.",

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AU - Ojamo, Heikki

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N2 - Different strategies were compared for the concentration of polygalacturonase, ß-glucosidase and ß-galactosidase produced by two strains of Aspergillus niger. Ultrafiltration was more successful than vacuum evaporation for all three enzymes but produced concentrates with high solids contents. The use of mineral adsorbents enabled almost complete separation and concentration of polygalacturonase and ß-glucosidase activities from one culture filtrate. Concentration of all three enzymes using adsorbents led to preparations with higher specific activities and lower solids contents than the corresponding ultrafiltration concentrates.

AB - Different strategies were compared for the concentration of polygalacturonase, ß-glucosidase and ß-galactosidase produced by two strains of Aspergillus niger. Ultrafiltration was more successful than vacuum evaporation for all three enzymes but produced concentrates with high solids contents. The use of mineral adsorbents enabled almost complete separation and concentration of polygalacturonase and ß-glucosidase activities from one culture filtrate. Concentration of all three enzymes using adsorbents led to preparations with higher specific activities and lower solids contents than the corresponding ultrafiltration concentrates.

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DO - 10.1007/978-94-009-0783-6_56

M3 - Chapter or book article

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