Droserone from cell cultures of Triphyophyllum peltatum (Dioncophyllaceae) and its biosynthetic origin

G. Bringmann (Corresponding Author), Heiko Rischer, M. Wohlfarth, J. Schlauer, L. Aké Assi

Research output: Contribution to journalArticleScientificpeer-review

33 Citations (Scopus)

Abstract

The growth and droserone content of callus cultures of Triphyophyllum peltatum grown in liquid 1/5 Linsmaier and Skoog medium was studied. During a lag phase in growth, droserone concentrations in the medium reached a value of 2.1 mg g−1 fr. wt. After this maximum value the concentration decreased slightly to 1.8 mg g−1 fr. wt., while the growth of the calli was enhanced (25% increase in fr. wt. within 7 days). Plumbagin and isoshinanolone were likewise present in the medium. By feeding 13C2-labelled acetate to the cultures the biosynthesis of droserone was elucidated. The incorporation of whole C2-units unambiguously shows its acetogenic origin and fits well in the biosynthetic scheme suggested for the structurally — and biogenetically — related naphthylisoquinoline alkaloids.
Original languageEnglish
Pages (from-to)339-343
Number of pages5
JournalPhytochemistry
Volume53
Issue number3
DOIs
Publication statusPublished - 2000
MoE publication typeNot Eligible

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Dioncophyllaceae
Triphyophyllum peltatum
plumbagin
callus culture
Cell culture
alkaloids
callus
cell culture
Cell Culture Techniques
acetates
Bony Callus
biosynthesis
liquids
Growth
Biosynthesis
Alkaloids
Acetates
Liquids

Cite this

Bringmann, G. ; Rischer, Heiko ; Wohlfarth, M. ; Schlauer, J. ; Aké Assi, L. / Droserone from cell cultures of Triphyophyllum peltatum (Dioncophyllaceae) and its biosynthetic origin. In: Phytochemistry. 2000 ; Vol. 53, No. 3. pp. 339-343.
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abstract = "The growth and droserone content of callus cultures of Triphyophyllum peltatum grown in liquid 1/5 Linsmaier and Skoog medium was studied. During a lag phase in growth, droserone concentrations in the medium reached a value of 2.1 mg g−1 fr. wt. After this maximum value the concentration decreased slightly to 1.8 mg g−1 fr. wt., while the growth of the calli was enhanced (25{\%} increase in fr. wt. within 7 days). Plumbagin and isoshinanolone were likewise present in the medium. By feeding 13C2-labelled acetate to the cultures the biosynthesis of droserone was elucidated. The incorporation of whole C2-units unambiguously shows its acetogenic origin and fits well in the biosynthetic scheme suggested for the structurally — and biogenetically — related naphthylisoquinoline alkaloids.",
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Droserone from cell cultures of Triphyophyllum peltatum (Dioncophyllaceae) and its biosynthetic origin. / Bringmann, G. (Corresponding Author); Rischer, Heiko; Wohlfarth, M.; Schlauer, J.; Aké Assi, L.

In: Phytochemistry, Vol. 53, No. 3, 2000, p. 339-343.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Droserone from cell cultures of Triphyophyllum peltatum (Dioncophyllaceae) and its biosynthetic origin

AU - Bringmann, G.

AU - Rischer, Heiko

AU - Wohlfarth, M.

AU - Schlauer, J.

AU - Aké Assi, L.

PY - 2000

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AB - The growth and droserone content of callus cultures of Triphyophyllum peltatum grown in liquid 1/5 Linsmaier and Skoog medium was studied. During a lag phase in growth, droserone concentrations in the medium reached a value of 2.1 mg g−1 fr. wt. After this maximum value the concentration decreased slightly to 1.8 mg g−1 fr. wt., while the growth of the calli was enhanced (25% increase in fr. wt. within 7 days). Plumbagin and isoshinanolone were likewise present in the medium. By feeding 13C2-labelled acetate to the cultures the biosynthesis of droserone was elucidated. The incorporation of whole C2-units unambiguously shows its acetogenic origin and fits well in the biosynthetic scheme suggested for the structurally — and biogenetically — related naphthylisoquinoline alkaloids.

U2 - 10.1016/S0031-9422(99)00543-9

DO - 10.1016/S0031-9422(99)00543-9

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VL - 53

SP - 339

EP - 343

JO - Phytochemistry

JF - Phytochemistry

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