Droserone from cell cultures of Triphyophyllum peltatum (Dioncophyllaceae) and its biosynthetic origin

G. Bringmann; Heiko Rischer; M. Wohlfarth; J. Schlauer; L. Aké Assi

doi:10.1016/S0031-9422(99)00543-9

Droserone from cell cultures of Triphyophyllum peltatum (Dioncophyllaceae) and its biosynthetic origin

G. Bringmann^*
, Heiko Rischer
, M. Wohlfarth
, J. Schlauer
, L. Aké Assi

^*Corresponding author for this work

Not published at VTT

Research output: Contribution to journal › Article › Scientific › peer-review

41 Citations (Scopus)

Abstract

The growth and droserone content of callus cultures of Triphyophyllum peltatum grown in liquid 1/5 Linsmaier and Skoog medium was studied. During a lag phase in growth, droserone concentrations in the medium reached a value of 2.1 mg g⁻¹ fr. wt. After this maximum value the concentration decreased slightly to 1.8 mg g⁻¹ fr. wt., while the growth of the calli was enhanced (25% increase in fr. wt. within 7 days). Plumbagin and isoshinanolone were likewise present in the medium. By feeding ¹³C₂-labelled acetate to the cultures the biosynthesis of droserone was elucidated. The incorporation of whole C₂-units unambiguously shows its acetogenic origin and fits well in the biosynthetic scheme suggested for the structurally — and biogenetically — related naphthylisoquinoline alkaloids.

Original language	English
Pages (from-to)	339-343
Number of pages	5
Journal	Phytochemistry
Volume	53
Issue number	3
DOIs	https://doi.org/10.1016/S0031-9422(99)00543-9
Publication status	Published - 2000
MoE publication type	Not Eligible

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title = "Droserone from cell cultures of Triphyophyllum peltatum (Dioncophyllaceae) and its biosynthetic origin",

abstract = "The growth and droserone content of callus cultures of Triphyophyllum peltatum grown in liquid 1/5 Linsmaier and Skoog medium was studied. During a lag phase in growth, droserone concentrations in the medium reached a value of 2.1 mg g−1 fr. wt. After this maximum value the concentration decreased slightly to 1.8 mg g−1 fr. wt., while the growth of the calli was enhanced (25\% increase in fr. wt. within 7 days). Plumbagin and isoshinanolone were likewise present in the medium. By feeding 13C2-labelled acetate to the cultures the biosynthesis of droserone was elucidated. The incorporation of whole C2-units unambiguously shows its acetogenic origin and fits well in the biosynthetic scheme suggested for the structurally — and biogenetically — related naphthylisoquinoline alkaloids.",

author = "G. Bringmann and Heiko Rischer and M. Wohlfarth and J. Schlauer and \{Ak{\'e} Assi\}, L.",

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doi = "10.1016/S0031-9422(99)00543-9",

language = "English",

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T1 - Droserone from cell cultures of Triphyophyllum peltatum (Dioncophyllaceae) and its biosynthetic origin

AU - Bringmann, G.

AU - Rischer, Heiko

AU - Wohlfarth, M.

AU - Schlauer, J.

AU - Aké Assi, L.

PY - 2000

Y1 - 2000

N2 - The growth and droserone content of callus cultures of Triphyophyllum peltatum grown in liquid 1/5 Linsmaier and Skoog medium was studied. During a lag phase in growth, droserone concentrations in the medium reached a value of 2.1 mg g−1 fr. wt. After this maximum value the concentration decreased slightly to 1.8 mg g−1 fr. wt., while the growth of the calli was enhanced (25% increase in fr. wt. within 7 days). Plumbagin and isoshinanolone were likewise present in the medium. By feeding 13C2-labelled acetate to the cultures the biosynthesis of droserone was elucidated. The incorporation of whole C2-units unambiguously shows its acetogenic origin and fits well in the biosynthetic scheme suggested for the structurally — and biogenetically — related naphthylisoquinoline alkaloids.

AB - The growth and droserone content of callus cultures of Triphyophyllum peltatum grown in liquid 1/5 Linsmaier and Skoog medium was studied. During a lag phase in growth, droserone concentrations in the medium reached a value of 2.1 mg g−1 fr. wt. After this maximum value the concentration decreased slightly to 1.8 mg g−1 fr. wt., while the growth of the calli was enhanced (25% increase in fr. wt. within 7 days). Plumbagin and isoshinanolone were likewise present in the medium. By feeding 13C2-labelled acetate to the cultures the biosynthesis of droserone was elucidated. The incorporation of whole C2-units unambiguously shows its acetogenic origin and fits well in the biosynthetic scheme suggested for the structurally — and biogenetically — related naphthylisoquinoline alkaloids.

U2 - 10.1016/S0031-9422(99)00543-9

DO - 10.1016/S0031-9422(99)00543-9

M3 - Article

SN - 0031-9422

VL - 53

SP - 339

EP - 343

JO - Phytochemistry

JF - Phytochemistry

IS - 3

ER -

Droserone from cell cultures of Triphyophyllum peltatum (Dioncophyllaceae) and its biosynthetic origin

Abstract

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