Dual mode diffraction phase microscopy for quantitative functional assessment of biological cells

N. A. Talaikova, Alexey P. Popov, A. L. Kalyanov, V. P. Ryabukho, I. V. Meglinski

Research output: Contribution to journalArticleScientificpeer-review

2 Citations (Scopus)

Abstract

A diffraction phase microscopy approach with a combined use of transmission and reflection imaging modes has been developed and applied for non-invasive quantitative assessment of the refractive index of red blood cells (RBCs). We present the theoretical background of signal formation for both imaging modes, accompanied by the results of experimental studies. We demonstrate that simultaneous use of the two modes has great potential for accurate assessment of the refractive index of biological cells, and we perform a reconstruction of spatial distribution of the refractive index of RBC in 3D.

Original languageEnglish
Article number105601
JournalLaser Physics Letters
Volume14
Issue number10
DOIs
Publication statusPublished - Oct 2017
MoE publication typeA1 Journal article-refereed

Keywords

  • cell imaging
  • diffraction phase microscopy
  • micro-interferometry
  • quantitative phase imaging
  • red blood cells
  • refractive index

Fingerprint Dive into the research topics of 'Dual mode diffraction phase microscopy for quantitative functional assessment of biological cells'. Together they form a unique fingerprint.

Cite this