Dynamics and interactions of parvoviral NS1 protein in the nucleus

Teemu O. Ihalainen, Einari A. Niskanen, Juulia Jylhävä, Tuomas Turpeinen, Johanna Rinne, Jussi Timonen, Maija Vihinen-Ranta (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

13 Citations (Scopus)

Abstract

Nuclear positioning and dynamic interactions of viral proteins with nuclear substructures play essential roles during infection with DNA viruses. Visualization of the intranuclear interactions and motility of the parvovirus replication protein (NS1) in living cells gives insight into specific parvovirus protein-cellular structure interactions. Confocal analysis of highly synchronized infected Norden Laboratory Feline Kidney cells showed accumulation of nuclear NS1 in discrete interchromosomal foci. NS1 fused with enhanced yellow fluorescence protein (NS1-EYFP) provided a marker in live cells for dynamics of NS1 traced by photobleaching techniques. Fluorescence Recovery after Photobleaching suggested that the NS1 protein is not freely diffusing but undergoes transient interactions with nuclear compartments. Fluorescence Loss in Photobleaching demonstrated for the first time the shuttling of a parvoviral protein between the nucleus and the cytoplasm as assayed with NS1-EYFP. Finally, time-lapse imaging of infected cells revealed that the intranuclear distribution of NS1-EYFP evolves dramatically starting from the formation of NS1 foci and proceeding to a homogenous distribution extending throughout the nucleus.
Original languageEnglish
Pages (from-to)1946-1959
JournalCellular Microbiology
Volume9
Issue number8
DOIs
Publication statusPublished - Aug 2007
MoE publication typeNot Eligible

Fingerprint

Fluorescence
Photobleaching
Proteins
DNA Virus Infections
Time-Lapse Imaging
Fluorescence Recovery After Photobleaching
Parvovirus
Felidae
Viral Proteins
Cellular Structures
Cytoplasm
Kidney

Cite this

Ihalainen, T. O., Niskanen, E. A., Jylhävä, J., Turpeinen, T., Rinne, J., Timonen, J., & Vihinen-Ranta, M. (2007). Dynamics and interactions of parvoviral NS1 protein in the nucleus. Cellular Microbiology, 9(8), 1946-1959. https://doi.org/10.1111/j.1462-5822.2007.00926.x
Ihalainen, Teemu O. ; Niskanen, Einari A. ; Jylhävä, Juulia ; Turpeinen, Tuomas ; Rinne, Johanna ; Timonen, Jussi ; Vihinen-Ranta, Maija. / Dynamics and interactions of parvoviral NS1 protein in the nucleus. In: Cellular Microbiology. 2007 ; Vol. 9, No. 8. pp. 1946-1959.
@article{cea6196428194f3ebb6b3e5ace73cb88,
title = "Dynamics and interactions of parvoviral NS1 protein in the nucleus",
abstract = "Nuclear positioning and dynamic interactions of viral proteins with nuclear substructures play essential roles during infection with DNA viruses. Visualization of the intranuclear interactions and motility of the parvovirus replication protein (NS1) in living cells gives insight into specific parvovirus protein-cellular structure interactions. Confocal analysis of highly synchronized infected Norden Laboratory Feline Kidney cells showed accumulation of nuclear NS1 in discrete interchromosomal foci. NS1 fused with enhanced yellow fluorescence protein (NS1-EYFP) provided a marker in live cells for dynamics of NS1 traced by photobleaching techniques. Fluorescence Recovery after Photobleaching suggested that the NS1 protein is not freely diffusing but undergoes transient interactions with nuclear compartments. Fluorescence Loss in Photobleaching demonstrated for the first time the shuttling of a parvoviral protein between the nucleus and the cytoplasm as assayed with NS1-EYFP. Finally, time-lapse imaging of infected cells revealed that the intranuclear distribution of NS1-EYFP evolves dramatically starting from the formation of NS1 foci and proceeding to a homogenous distribution extending throughout the nucleus.",
author = "Ihalainen, {Teemu O.} and Niskanen, {Einari A.} and Juulia Jylh{\"a}v{\"a} and Tuomas Turpeinen and Johanna Rinne and Jussi Timonen and Maija Vihinen-Ranta",
year = "2007",
month = "8",
doi = "10.1111/j.1462-5822.2007.00926.x",
language = "English",
volume = "9",
pages = "1946--1959",
journal = "Cellular Microbiology",
issn = "1462-5814",
publisher = "Wiley-Blackwell",
number = "8",

}

Ihalainen, TO, Niskanen, EA, Jylhävä, J, Turpeinen, T, Rinne, J, Timonen, J & Vihinen-Ranta, M 2007, 'Dynamics and interactions of parvoviral NS1 protein in the nucleus', Cellular Microbiology, vol. 9, no. 8, pp. 1946-1959. https://doi.org/10.1111/j.1462-5822.2007.00926.x

Dynamics and interactions of parvoviral NS1 protein in the nucleus. / Ihalainen, Teemu O.; Niskanen, Einari A.; Jylhävä, Juulia; Turpeinen, Tuomas; Rinne, Johanna; Timonen, Jussi; Vihinen-Ranta, Maija (Corresponding Author).

In: Cellular Microbiology, Vol. 9, No. 8, 08.2007, p. 1946-1959.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Dynamics and interactions of parvoviral NS1 protein in the nucleus

AU - Ihalainen, Teemu O.

AU - Niskanen, Einari A.

AU - Jylhävä, Juulia

AU - Turpeinen, Tuomas

AU - Rinne, Johanna

AU - Timonen, Jussi

AU - Vihinen-Ranta, Maija

PY - 2007/8

Y1 - 2007/8

N2 - Nuclear positioning and dynamic interactions of viral proteins with nuclear substructures play essential roles during infection with DNA viruses. Visualization of the intranuclear interactions and motility of the parvovirus replication protein (NS1) in living cells gives insight into specific parvovirus protein-cellular structure interactions. Confocal analysis of highly synchronized infected Norden Laboratory Feline Kidney cells showed accumulation of nuclear NS1 in discrete interchromosomal foci. NS1 fused with enhanced yellow fluorescence protein (NS1-EYFP) provided a marker in live cells for dynamics of NS1 traced by photobleaching techniques. Fluorescence Recovery after Photobleaching suggested that the NS1 protein is not freely diffusing but undergoes transient interactions with nuclear compartments. Fluorescence Loss in Photobleaching demonstrated for the first time the shuttling of a parvoviral protein between the nucleus and the cytoplasm as assayed with NS1-EYFP. Finally, time-lapse imaging of infected cells revealed that the intranuclear distribution of NS1-EYFP evolves dramatically starting from the formation of NS1 foci and proceeding to a homogenous distribution extending throughout the nucleus.

AB - Nuclear positioning and dynamic interactions of viral proteins with nuclear substructures play essential roles during infection with DNA viruses. Visualization of the intranuclear interactions and motility of the parvovirus replication protein (NS1) in living cells gives insight into specific parvovirus protein-cellular structure interactions. Confocal analysis of highly synchronized infected Norden Laboratory Feline Kidney cells showed accumulation of nuclear NS1 in discrete interchromosomal foci. NS1 fused with enhanced yellow fluorescence protein (NS1-EYFP) provided a marker in live cells for dynamics of NS1 traced by photobleaching techniques. Fluorescence Recovery after Photobleaching suggested that the NS1 protein is not freely diffusing but undergoes transient interactions with nuclear compartments. Fluorescence Loss in Photobleaching demonstrated for the first time the shuttling of a parvoviral protein between the nucleus and the cytoplasm as assayed with NS1-EYFP. Finally, time-lapse imaging of infected cells revealed that the intranuclear distribution of NS1-EYFP evolves dramatically starting from the formation of NS1 foci and proceeding to a homogenous distribution extending throughout the nucleus.

U2 - 10.1111/j.1462-5822.2007.00926.x

DO - 10.1111/j.1462-5822.2007.00926.x

M3 - Article

C2 - 17419720

VL - 9

SP - 1946

EP - 1959

JO - Cellular Microbiology

JF - Cellular Microbiology

SN - 1462-5814

IS - 8

ER -