Effect of a milling pre-treatment on the enzymatic hydrolysis of carbohydrates in brewer's spent grain

Piritta Niemi (Corresponding Author), Craig B. Faulds, Juhani Sibakov, Ulla Holopainen, Kaisa Poutanen, Johanna Buchert

Research output: Contribution to journalArticleScientificpeer-review

31 Citations (Scopus)


Millions of tonnes of brewer's spent grain (BSG) are annually produced worldwide as a by-product of the brewing industry. BSG has the potential to be a valuable source of food, chemicals and energy if cost-efficient fractionation methods can be developed. A 2-fold improvement in carbohydrate solubilisation could be achieved through the introduction of a milling step prior to enzymatic hydrolysis. Course and fine milled fractions were characterized by particle size distribution and light microscopy. Fine milling decreased particle size down to the micron level and this in turn improved the carbohydrate solubility yield by a multi-enzyme mixture from 23% up to 45%. Carbohydrate solubilisation could be further increased through the supplementation of this enzyme preparation with additional cellulases. The physical degradation caused by the milling also liberated soluble carbohydrates without the requirement of any enzymatic treatment.
Original languageEnglish
Pages (from-to)155-160
Number of pages6
JournalBioresource Technology
Publication statusPublished - Jul 2012
MoE publication typeA1 Journal article-refereed


  • Biotechnology
  • Biotechnology: methods
  • Carbohydrate Metabolism
  • Carbohydrates
  • Carbohydrates: chemistry
  • Cereals
  • Cereals: metabolism
  • Enzymes
  • Enzymes: metabolism
  • Hydrolysis
  • Industrial Waste
  • Industrial Waste: analysis
  • Particle Size
  • Solubility
  • Time Factors
  • Waste Products
  • Waste Products: analysis

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