Effect of a milling pre-treatment on the enzymatic hydrolysis of carbohydrates in brewer's spent grain

Piritta Niemi (Corresponding Author), Craig B. Faulds, Juhani Sibakov, Ulla Holopainen, Kaisa Poutanen, Johanna Buchert

Research output: Contribution to journalArticleScientificpeer-review

24 Citations (Scopus)

Abstract

Millions of tonnes of brewer's spent grain (BSG) are annually produced worldwide as a by-product of the brewing industry. BSG has the potential to be a valuable source of food, chemicals and energy if cost-efficient fractionation methods can be developed. A 2-fold improvement in carbohydrate solubilisation could be achieved through the introduction of a milling step prior to enzymatic hydrolysis. Course and fine milled fractions were characterized by particle size distribution and light microscopy. Fine milling decreased particle size down to the micron level and this in turn improved the carbohydrate solubility yield by a multi-enzyme mixture from 23% up to 45%. Carbohydrate solubilisation could be further increased through the supplementation of this enzyme preparation with additional cellulases. The physical degradation caused by the milling also liberated soluble carbohydrates without the requirement of any enzymatic treatment.
Original languageEnglish
Pages (from-to)155-160
Number of pages6
JournalBioresource Technology
Volume116
DOIs
Publication statusPublished - Jul 2012
MoE publication typeA1 Journal article-refereed

Fingerprint

Enzymatic hydrolysis
Carbohydrates
carbohydrate
hydrolysis
solubilization
brewing industry
Enzymes
particle size
Brewing
enzyme
Cellulases
Fractionation
Particle size analysis
Optical microscopy
Byproducts
microscopy
solubility
fractionation
Solubility
Particle size

Keywords

  • Biotechnology
  • Biotechnology: methods
  • Carbohydrate Metabolism
  • Carbohydrates
  • Carbohydrates: chemistry
  • Cereals
  • Cereals: metabolism
  • Enzymes
  • Enzymes: metabolism
  • Hydrolysis
  • Industrial Waste
  • Industrial Waste: analysis
  • Particle Size
  • Solubility
  • Time Factors
  • Waste Products
  • Waste Products: analysis

Cite this

Niemi, Piritta ; Faulds, Craig B. ; Sibakov, Juhani ; Holopainen, Ulla ; Poutanen, Kaisa ; Buchert, Johanna. / Effect of a milling pre-treatment on the enzymatic hydrolysis of carbohydrates in brewer's spent grain. In: Bioresource Technology. 2012 ; Vol. 116. pp. 155-160.
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Effect of a milling pre-treatment on the enzymatic hydrolysis of carbohydrates in brewer's spent grain. / Niemi, Piritta (Corresponding Author); Faulds, Craig B.; Sibakov, Juhani; Holopainen, Ulla; Poutanen, Kaisa; Buchert, Johanna.

In: Bioresource Technology, Vol. 116, 07.2012, p. 155-160.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Effect of a milling pre-treatment on the enzymatic hydrolysis of carbohydrates in brewer's spent grain

AU - Niemi, Piritta

AU - Faulds, Craig B.

AU - Sibakov, Juhani

AU - Holopainen, Ulla

AU - Poutanen, Kaisa

AU - Buchert, Johanna

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AB - Millions of tonnes of brewer's spent grain (BSG) are annually produced worldwide as a by-product of the brewing industry. BSG has the potential to be a valuable source of food, chemicals and energy if cost-efficient fractionation methods can be developed. A 2-fold improvement in carbohydrate solubilisation could be achieved through the introduction of a milling step prior to enzymatic hydrolysis. Course and fine milled fractions were characterized by particle size distribution and light microscopy. Fine milling decreased particle size down to the micron level and this in turn improved the carbohydrate solubility yield by a multi-enzyme mixture from 23% up to 45%. Carbohydrate solubilisation could be further increased through the supplementation of this enzyme preparation with additional cellulases. The physical degradation caused by the milling also liberated soluble carbohydrates without the requirement of any enzymatic treatment.

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KW - Biotechnology: methods

KW - Carbohydrate Metabolism

KW - Carbohydrates

KW - Carbohydrates: chemistry

KW - Cereals

KW - Cereals: metabolism

KW - Enzymes

KW - Enzymes: metabolism

KW - Hydrolysis

KW - Industrial Waste

KW - Industrial Waste: analysis

KW - Particle Size

KW - Solubility

KW - Time Factors

KW - Waste Products

KW - Waste Products: analysis

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