@article{f76fa0edf65940ae8ffe3549810db78e,
title = "Effect of cellulase family and structure on modification of wood fibres at high consistency",
abstract = "Enzymatic modification of bleached softwood kraft fibres for improved fibre reactivity was studied at high (20% w/w) and low (1% w/w) dry matter content. The role of enzyme family and structure in fibre modification was assessed using endoglucanases from three structurally different glycoside hydrolase (GH) families (5, 7 and 45) with and without a carbohydrate binding module (CBM). Based on the amount of dissolved sugars, enzyme action at high consistency was about sixfold higher compared to a fibre treatment at low consistency. The GH45 endoglucanase was found to be most specific in acting on pulp cellulose whereas the family 5 and 7 endoglucanases had activity also on pulp hemicelluloses. The GH45 endoglucanase was found to be most efficient in reducing molecular weight and viscosity of the pulp. In addition, treatment with the GH45 endoglucanase resulted in the highest micropore volume in fibres and thus an increase in cellulose accessibility. The increased accessibility could be seen as decreased dissolution time in cupriethylenediamine using recently developed analytical techniques: viscometric analysis and microscopic video analysis. At high consistency, CBM was not promoting enzyme action, although CBMs are known to be beneficial at low dry matter conditions. Graphical abstract: [Figure not available: see fulltext.].",
keywords = "Dissolution, Endoglucanase, Enzyme, Fibre, Fibre reactivity, High consistency",
author = "Jenni Rahikainen and Sara Ceccherini and Matthieu Molinier and Ulla Holopainen-Mantila and Mehedi Reza and Saija V{\"a}is{\"a}nen and Terhi Puranen and Kristiina Kruus and Tapani Vuorinen and Thaddeus Maloney and Anna Suurn{\"a}kki and Stina Gr{\"o}nqvist",
note = "Funding Information: Open access funding provided by Technical Research Centre of Finland (VTT). Funding from the Academy of Finland (Decision No 277791) for the Porofibre consortium project is highly acknowledged. This work was a part of the Academy of Finland{\textquoteright}s Flagship Programme under Projects Nos. 318890 and 318891 (Competence Center for Materials Bioeconomy, FinnCERES). Support from the Foundation for Aalto University Science and Technology and collaboration with the ACel research programme funded by Business Finland (former Finnish Funding Agency for Technology and Innovation, Tekes) as well as industrial partners is highly appreciated. Alexey Khakalo (Aalto University) and the ACel programme are acknowledged for the compositional data of the bleached softwood kraft pulp. Analytical expertise of Atte Mikkelson and technical assistance of Mariitta Svanberg, Liisa {\"A}n{\"a}k{\"a}inen and Ritva Heinonen is gratefully acknowledged. Funding Information: Acknowledgments Open access funding provided by Technical Research Centre of Finland (VTT). Funding from the Academy of Finland (Decision No 277791) for the Porofibre consortium project is highly acknowledged. This work was a part of the Academy of Finland{\textquoteright}s Flagship Programme under Projects Nos. 318890 and 318891 (Competence Center for Materials Bioeconomy, FinnCERES). Support from the Foundation for Aalto University Science and Technology and collaboration with the ACel research programme funded by Business Finland (former Finnish Funding Agency for Technology and Innovation, Tekes) as well as industrial partners is highly appreciated. Alexey Khakalo (Aalto University) and the ACel programme are acknowledged for the compositional data of the bleached softwood kraft pulp. Analytical expertise of Atte Mikkelson and technical assistance of Mariitta Svanberg, Liisa {\"A}n{\"a}k{\"a}inen and Ritva Heinonen is gratefully acknowledged. Publisher Copyright: {\textcopyright} 2019, The Author(s).",
year = "2019",
month = may,
day = "30",
doi = "10.1007/s10570-019-02424-x",
language = "English",
volume = "26",
pages = "5085--5103",
journal = "Cellulose",
issn = "0969-0239",
publisher = "Springer",
number = "8",
}