Effect of EDTA on Salmonella enterica serovar Typhimurium involves a component not assignable to lipopolysaccharide release

Hanna-Leena Alakomi (Corresponding Author), Maria Saarela, Ilkka Helander

Research output: Contribution to journalArticleScientificpeer-review

43 Citations (Scopus)

Abstract

The effect of EDTA on Salmonella enterica serovar Typhimurium was studied in different growth phases with cells grown with or without Ca2+ and Mg2+ supplementation. EDTA affected the outer membrane much more strongly in the early exponential phase than in the mid- or late exponential phase, as indicated by uptake of 1-N-phenylnaphthylamine (a nonpolar hydrophobic probe, M r 219), and detergent (SDS) susceptibility. This effect was, however, not paralleled by LPS release (determined by measuring LPS-specific fatty acids or 14C-labelled LPS in cell-free supernatants, per a standardized cell density), which remained unchanged as a function of the growth curve. The conclusion from these results is that in the early exponential phase the effect of EDTA in S. enterica involves a component that is independent of LPS release.
Original languageEnglish
Pages (from-to)2015-2021
Number of pages7
JournalMicrobiology
Volume149
Issue number8
DOIs
Publication statusPublished - 2003
MoE publication typeA1 Journal article-refereed

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Salmonella enterica
Edetic Acid
Lipopolysaccharides
N-phenyl-1-naphthylamine
Growth
Detergents
Fatty Acids
Cell Count
Membranes
Serogroup

Keywords

  • EDTA
  • Salmonella
  • LPS

Cite this

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title = "Effect of EDTA on Salmonella enterica serovar Typhimurium involves a component not assignable to lipopolysaccharide release",
abstract = "The effect of EDTA on Salmonella enterica serovar Typhimurium was studied in different growth phases with cells grown with or without Ca2+ and Mg2+ supplementation. EDTA affected the outer membrane much more strongly in the early exponential phase than in the mid- or late exponential phase, as indicated by uptake of 1-N-phenylnaphthylamine (a nonpolar hydrophobic probe, M r 219), and detergent (SDS) susceptibility. This effect was, however, not paralleled by LPS release (determined by measuring LPS-specific fatty acids or 14C-labelled LPS in cell-free supernatants, per a standardized cell density), which remained unchanged as a function of the growth curve. The conclusion from these results is that in the early exponential phase the effect of EDTA in S. enterica involves a component that is independent of LPS release.",
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Effect of EDTA on Salmonella enterica serovar Typhimurium involves a component not assignable to lipopolysaccharide release. / Alakomi, Hanna-Leena (Corresponding Author); Saarela, Maria; Helander, Ilkka.

In: Microbiology, Vol. 149, No. 8, 2003, p. 2015-2021.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Effect of EDTA on Salmonella enterica serovar Typhimurium involves a component not assignable to lipopolysaccharide release

AU - Alakomi, Hanna-Leena

AU - Saarela, Maria

AU - Helander, Ilkka

PY - 2003

Y1 - 2003

N2 - The effect of EDTA on Salmonella enterica serovar Typhimurium was studied in different growth phases with cells grown with or without Ca2+ and Mg2+ supplementation. EDTA affected the outer membrane much more strongly in the early exponential phase than in the mid- or late exponential phase, as indicated by uptake of 1-N-phenylnaphthylamine (a nonpolar hydrophobic probe, M r 219), and detergent (SDS) susceptibility. This effect was, however, not paralleled by LPS release (determined by measuring LPS-specific fatty acids or 14C-labelled LPS in cell-free supernatants, per a standardized cell density), which remained unchanged as a function of the growth curve. The conclusion from these results is that in the early exponential phase the effect of EDTA in S. enterica involves a component that is independent of LPS release.

AB - The effect of EDTA on Salmonella enterica serovar Typhimurium was studied in different growth phases with cells grown with or without Ca2+ and Mg2+ supplementation. EDTA affected the outer membrane much more strongly in the early exponential phase than in the mid- or late exponential phase, as indicated by uptake of 1-N-phenylnaphthylamine (a nonpolar hydrophobic probe, M r 219), and detergent (SDS) susceptibility. This effect was, however, not paralleled by LPS release (determined by measuring LPS-specific fatty acids or 14C-labelled LPS in cell-free supernatants, per a standardized cell density), which remained unchanged as a function of the growth curve. The conclusion from these results is that in the early exponential phase the effect of EDTA in S. enterica involves a component that is independent of LPS release.

KW - EDTA

KW - Salmonella

KW - LPS

U2 - 10.1099/mic.0.26312-0

DO - 10.1099/mic.0.26312-0

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VL - 149

SP - 2015

EP - 2021

JO - Microbiology

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SN - 1350-0872

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