Effect of hydrolyzing enzymes on wheat bran cell wall integrity and protein solubility

Elisa Arte, Kati Katina, Ulla Holopainen-Mantila, Emilia Nordlund

Research output: Contribution to journalArticleScientificpeer-review

6 Citations (Scopus)

Abstract

Wheat bran contains good quality protein, but given its location inside aleurone cells, this protein has restricted digestibility. The aim of this work was to liberate and solubilize wheat bran proteins via cell wall degradation by using carbohydrate-hydrolyzing and proteolytic enzymes without causing extensive protein hydrolysis. Bran incubated with water (without added enzymes) for 16 h increased the solubilized organic nitrogen content from 14.0 to 42.8%. Enzymes with solely carbohydrate-hydrolyzing activity increased the water-soluble pentosan and reducing sugar contents but did not significantly increase protein solubilization or protein release from the aleurone cells. Enzymes with proteolytic activity significantly increased the solubilization of protein to 58.2% already at 4 h. Significant protein hydrolysis was detected with a high dosage of protease. However, based on light microscopy, the enzymatic treatment mainly modified the proteins in the subaleurone layer, and it was less effective on proteins inside the aleurone cells. With optimized protease treatment (3 h, 35°C, and 550 nkat/g), effective protein solubilization (>48%) without extensive protein hydrolysis (free amino nitrogen content
Original languageEnglish
Pages (from-to)162-171
JournalCereal Chemistry
Volume93
Issue number2
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

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protein solubility
Dietary Fiber
wheat bran
Solubility
Cell Wall
Cells
cell walls
Enzymes
enzymes
Proteins
proteins
aleurone cells
Peptide Hydrolases
solubilization
Hydrolysis
hydrolysis
nitrogen content
Nitrogen
proteinases
Carbohydrates

Cite this

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title = "Effect of hydrolyzing enzymes on wheat bran cell wall integrity and protein solubility",
abstract = "Wheat bran contains good quality protein, but given its location inside aleurone cells, this protein has restricted digestibility. The aim of this work was to liberate and solubilize wheat bran proteins via cell wall degradation by using carbohydrate-hydrolyzing and proteolytic enzymes without causing extensive protein hydrolysis. Bran incubated with water (without added enzymes) for 16 h increased the solubilized organic nitrogen content from 14.0 to 42.8{\%}. Enzymes with solely carbohydrate-hydrolyzing activity increased the water-soluble pentosan and reducing sugar contents but did not significantly increase protein solubilization or protein release from the aleurone cells. Enzymes with proteolytic activity significantly increased the solubilization of protein to 58.2{\%} already at 4 h. Significant protein hydrolysis was detected with a high dosage of protease. However, based on light microscopy, the enzymatic treatment mainly modified the proteins in the subaleurone layer, and it was less effective on proteins inside the aleurone cells. With optimized protease treatment (3 h, 35°C, and 550 nkat/g), effective protein solubilization (>48{\%}) without extensive protein hydrolysis (free amino nitrogen content",
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Effect of hydrolyzing enzymes on wheat bran cell wall integrity and protein solubility. / Arte, Elisa; Katina, Kati; Holopainen-Mantila, Ulla; Nordlund, Emilia.

In: Cereal Chemistry, Vol. 93, No. 2, 2016, p. 162-171.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Effect of hydrolyzing enzymes on wheat bran cell wall integrity and protein solubility

AU - Arte, Elisa

AU - Katina, Kati

AU - Holopainen-Mantila, Ulla

AU - Nordlund, Emilia

PY - 2016

Y1 - 2016

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AB - Wheat bran contains good quality protein, but given its location inside aleurone cells, this protein has restricted digestibility. The aim of this work was to liberate and solubilize wheat bran proteins via cell wall degradation by using carbohydrate-hydrolyzing and proteolytic enzymes without causing extensive protein hydrolysis. Bran incubated with water (without added enzymes) for 16 h increased the solubilized organic nitrogen content from 14.0 to 42.8%. Enzymes with solely carbohydrate-hydrolyzing activity increased the water-soluble pentosan and reducing sugar contents but did not significantly increase protein solubilization or protein release from the aleurone cells. Enzymes with proteolytic activity significantly increased the solubilization of protein to 58.2% already at 4 h. Significant protein hydrolysis was detected with a high dosage of protease. However, based on light microscopy, the enzymatic treatment mainly modified the proteins in the subaleurone layer, and it was less effective on proteins inside the aleurone cells. With optimized protease treatment (3 h, 35°C, and 550 nkat/g), effective protein solubilization (>48%) without extensive protein hydrolysis (free amino nitrogen content

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