Effect of hydrothermal pretreatment severity on lignin inhibition in the enzymatic hydrolysis of lignocellulosic biomass

Miriam Kellock, Heng Zhang, Kaisa Marjamaa, Tarja Tamminen, Claus Felby, Kristiina Kruus

Research output: Contribution to conferenceConference articleScientific

Abstract

It is frequently seen that enzymatic hydrolysis of carbohydrates in lignocellulosic biomass is inhibited by non-productive adsorption of enzymes on the lignin surface. To compensate partial enzyme adsorption, enzyme dosages have generally remained high and enzymes continue to be a major cost in sugar production from lignocellulosic biomass. The high process cost in biorefineries could partially be overcome by minimizing non-productive binding and thereby promoting the reuse of enzymes. Thermochemical pretreatment is predominant for lignocellulosics to make the cell wall carbohydrates more susceptible to enzyme degradation. Our previous work has shown that pretreatment of lignocellulose alteres the lignin chemistry and enhances enzyme adsorption onto lignin. The present study further examines the effects of thermochemical pretreatment on lignin chemistry and enzyme adsorption. Spruce and wheat straw were pretreated under hydrothermal conditions from 180 to 220 °C in the presence or absence of a dilute acid catalyst (H2SO4). Lignins under different pretreatment severities were isolated and analyzed in order to identify the changes introduced by the pretreatment. This paper will discuss the effect of pretreatment severity on enzymatic hydrolysis and non-productive adsorption of enzymes onto lignin.
Original languageEnglish
Publication statusPublished - 2016
EventSBFC Symposium on Biotechnology for Fuels and Chemicals - Baltimore, United States
Duration: 24 Apr 201628 Apr 2016

Conference

ConferenceSBFC Symposium on Biotechnology for Fuels and Chemicals
CountryUnited States
CityBaltimore
Period24/04/1628/04/16

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enzymatic hydrolysis
lignin
pretreatment
biomass
enzymes
adsorption
chemistry
carbohydrates
biorefining
lignocellulose
wheat straw
catalysts
Picea
cell walls
sugars
degradation
acids
dosage

Cite this

Kellock, M., Zhang, H., Marjamaa, K., Tamminen, T., Felby, C., & Kruus, K. (2016). Effect of hydrothermal pretreatment severity on lignin inhibition in the enzymatic hydrolysis of lignocellulosic biomass. Paper presented at SBFC Symposium on Biotechnology for Fuels and Chemicals, Baltimore, United States.
Kellock, Miriam ; Zhang, Heng ; Marjamaa, Kaisa ; Tamminen, Tarja ; Felby, Claus ; Kruus, Kristiina. / Effect of hydrothermal pretreatment severity on lignin inhibition in the enzymatic hydrolysis of lignocellulosic biomass. Paper presented at SBFC Symposium on Biotechnology for Fuels and Chemicals, Baltimore, United States.
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title = "Effect of hydrothermal pretreatment severity on lignin inhibition in the enzymatic hydrolysis of lignocellulosic biomass",
abstract = "It is frequently seen that enzymatic hydrolysis of carbohydrates in lignocellulosic biomass is inhibited by non-productive adsorption of enzymes on the lignin surface. To compensate partial enzyme adsorption, enzyme dosages have generally remained high and enzymes continue to be a major cost in sugar production from lignocellulosic biomass. The high process cost in biorefineries could partially be overcome by minimizing non-productive binding and thereby promoting the reuse of enzymes. Thermochemical pretreatment is predominant for lignocellulosics to make the cell wall carbohydrates more susceptible to enzyme degradation. Our previous work has shown that pretreatment of lignocellulose alteres the lignin chemistry and enhances enzyme adsorption onto lignin. The present study further examines the effects of thermochemical pretreatment on lignin chemistry and enzyme adsorption. Spruce and wheat straw were pretreated under hydrothermal conditions from 180 to 220 °C in the presence or absence of a dilute acid catalyst (H2SO4). Lignins under different pretreatment severities were isolated and analyzed in order to identify the changes introduced by the pretreatment. This paper will discuss the effect of pretreatment severity on enzymatic hydrolysis and non-productive adsorption of enzymes onto lignin.",
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year = "2016",
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Kellock, M, Zhang, H, Marjamaa, K, Tamminen, T, Felby, C & Kruus, K 2016, 'Effect of hydrothermal pretreatment severity on lignin inhibition in the enzymatic hydrolysis of lignocellulosic biomass' Paper presented at SBFC Symposium on Biotechnology for Fuels and Chemicals, Baltimore, United States, 24/04/16 - 28/04/16, .

Effect of hydrothermal pretreatment severity on lignin inhibition in the enzymatic hydrolysis of lignocellulosic biomass. / Kellock, Miriam; Zhang, Heng; Marjamaa, Kaisa; Tamminen, Tarja; Felby, Claus; Kruus, Kristiina.

2016. Paper presented at SBFC Symposium on Biotechnology for Fuels and Chemicals, Baltimore, United States.

Research output: Contribution to conferenceConference articleScientific

TY - CONF

T1 - Effect of hydrothermal pretreatment severity on lignin inhibition in the enzymatic hydrolysis of lignocellulosic biomass

AU - Kellock, Miriam

AU - Zhang, Heng

AU - Marjamaa, Kaisa

AU - Tamminen, Tarja

AU - Felby, Claus

AU - Kruus, Kristiina

PY - 2016

Y1 - 2016

N2 - It is frequently seen that enzymatic hydrolysis of carbohydrates in lignocellulosic biomass is inhibited by non-productive adsorption of enzymes on the lignin surface. To compensate partial enzyme adsorption, enzyme dosages have generally remained high and enzymes continue to be a major cost in sugar production from lignocellulosic biomass. The high process cost in biorefineries could partially be overcome by minimizing non-productive binding and thereby promoting the reuse of enzymes. Thermochemical pretreatment is predominant for lignocellulosics to make the cell wall carbohydrates more susceptible to enzyme degradation. Our previous work has shown that pretreatment of lignocellulose alteres the lignin chemistry and enhances enzyme adsorption onto lignin. The present study further examines the effects of thermochemical pretreatment on lignin chemistry and enzyme adsorption. Spruce and wheat straw were pretreated under hydrothermal conditions from 180 to 220 °C in the presence or absence of a dilute acid catalyst (H2SO4). Lignins under different pretreatment severities were isolated and analyzed in order to identify the changes introduced by the pretreatment. This paper will discuss the effect of pretreatment severity on enzymatic hydrolysis and non-productive adsorption of enzymes onto lignin.

AB - It is frequently seen that enzymatic hydrolysis of carbohydrates in lignocellulosic biomass is inhibited by non-productive adsorption of enzymes on the lignin surface. To compensate partial enzyme adsorption, enzyme dosages have generally remained high and enzymes continue to be a major cost in sugar production from lignocellulosic biomass. The high process cost in biorefineries could partially be overcome by minimizing non-productive binding and thereby promoting the reuse of enzymes. Thermochemical pretreatment is predominant for lignocellulosics to make the cell wall carbohydrates more susceptible to enzyme degradation. Our previous work has shown that pretreatment of lignocellulose alteres the lignin chemistry and enhances enzyme adsorption onto lignin. The present study further examines the effects of thermochemical pretreatment on lignin chemistry and enzyme adsorption. Spruce and wheat straw were pretreated under hydrothermal conditions from 180 to 220 °C in the presence or absence of a dilute acid catalyst (H2SO4). Lignins under different pretreatment severities were isolated and analyzed in order to identify the changes introduced by the pretreatment. This paper will discuss the effect of pretreatment severity on enzymatic hydrolysis and non-productive adsorption of enzymes onto lignin.

M3 - Conference article

ER -

Kellock M, Zhang H, Marjamaa K, Tamminen T, Felby C, Kruus K. Effect of hydrothermal pretreatment severity on lignin inhibition in the enzymatic hydrolysis of lignocellulosic biomass. 2016. Paper presented at SBFC Symposium on Biotechnology for Fuels and Chemicals, Baltimore, United States.